Modification of the laser correlation spectrometer for analysis of biological fluids

E.N. Velichko*, E.K. Nepomnyashchaya**, E.T. Aksenov***

Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia

E-mail: *velichko-spbstu@yandex.ru, **elina.nep@gmail.com, ***et.aksenov@gmail.com

Received November 24, 2017

Full text PDF

Abstract

Modifications of the laser correlation spectrometer system developed for the analysis of biological fluids have been discussed. Parameters of the proposed laser correlation spectrometer allowing to determine the sizes of nanoparticles in biological fluids, which are highly polydisperse solutions, to within 0.5 nm have been presented. We have explained the use of optical fiber in the spectrometer circuit as a system for collecting scattered radiation. Furthermore, we calculated the parameters of the discretization of the registered signals. It is for the correct selection of the ADC-converter. The results of the experiment showing significant differences in the size composition of donor blood serum proteins have been presented. Thus, the possibility for analyzing the composition of the blood serum of various donors and for obtaining diagnostic data has been proved.

Keywords: laser correlation spectroscopy, immune system, molecular analysis, blood serum

Acknowledgments. We are grateful to Tat'yana Aleksandrovna Bogomaz for her invaluable help during the experimental data analysis and for kindly providing us with the samples.

Figure Captions

Fig. 1. The layout of the laser correlation spectrometer: 1 – laser; 2 – focusing lens; 3 – container with the studied object; 4 – optical fiber; 5 – PMT; 6 – AD converter; 7 – computer.

Fig. 2. Data obtained as a result of the measurements of particle distribution by size in the blood serum solution of a male donor.

Fig. 3. Data obtained as a result of the measurements of particle distribution by size in the blood serum solution of a female donor.

References

1. Borisov A.G., Savchenko A.A., Sokolovskaya V.K. The morbidity related to disorders of immune system function, as exemplified by Krasnoyarsk krai. Zdravookhr. Ross. Fed., 2014, vol. 58, no. 6, pp. 38–41. (In Russian)

2. Zuev A.L., Mishlanov V.Yu., Sudakova A.I., Shakirov N.V. Analysis of ionic, molecular, and cellular mechanisms of electrical impedance formation in biological fluids and tissues. Vestn. Permsk. Nauchn. Ural. Otd. Ross. Akad. Nauk, 2014, no. 2, pp. 69–78. (In Russian)

3. Sherstneva N.V., Zakirova L.R., Shaposhnikov A.A., Shentseva E.A., Shevchenko T.S. Testing of the biochemical blood composition of healthy people and changing parameters during infection by the human immunodeficiency virus. Nauchn. Vedomosti, Ser. Med. Farm., 2017, no. 5, pp. 59–62. (In Russian)

4. Some D., Kenrick S. Characterization of protein-protein interactions via static and dynamic light scattering. In: Cai J. (Ed.) Protein Interactions. 2012, pp. 401–426. doi: 10.5772/37240.

5. Xu R. Light scattering: A review of particle characterization applications. Particuology, 2014, vol. 18, pp. 11-21. doi: 10.1016/j.partic.2014.05.002.

6. Nepomnyashchaya E.K., Velichko E.N., Aksenov E.T., Bogomaz T.A. Applying laser correlation spectroscopy for studying biological suspensions. Opt. Zh., 2015, vol. 82, no. 3, pp. 43–48. (In Russian)

7. Stetefeld J., McKenna S.A., Patel T.R. Dynamic light scattering: a practical guide and applications in biomedical sciences. Biophys. Rev., 2016, vol. 8, no. 4, pp. 409–427. doi: 0.1007/s12551-016-0218-6.

8. Nepomnyashchaya E.K., Savchenko E.A., Velichko E.N., Aksenov E.T. Investigation of albumin-fullerenol interaction by laser correlation spectroscopy: The algorithm. J. Biomed. Photon. Eng., 2016, vol. 2, no. 4, art. 040309, pp. 1–6. doi: 10.18287/JBPE16.02.040309.

9. Nepomniashchaia E.K., Velichko E.N., Aksenov E.T. Solution of inverse problem of laser correlation spectroscopy by regularization method. Univ. Nauchn. Zh., 2015, no. 13, pp. 13–21.

10. Nepomniashchaia E.K., Velichko E.N., Aksenov E.T. Inverse problem of laser correlation spectroscopy for analysis of polydisperse solutions of nanoparticles. J. Phys.: Conf. Ser., 2016, vol. 769, no. 1, art. 012025, pp. 1–5. doi: 10.1088/1742-6596/769/1/012025.

11. Novikov P.D., Konevalova N.Yu., Titova N.D. The principles of evaluation of immune status and diagnostic of immunodeficiency diseases. Immunopathol., Allergol., Infectol., 2005, no 2, pp. 8–22.

12. Nepomnyashchaya E., Zabalueva Z., Velichko E., Aksenov E. Modifications of laser correlation spectrometer for investigation of biological fluids. EPJ Web Conf., 2017, vol. 161, art. 02017, pp. 1–2. doi: 10.1051/epjconf/201716102017.

For citation: Velichko E.N., Nepomnyashchaya E.K., Aksenov E.T. Modification of the  laser  correlation  spectrometer  for  analysis  of  biological  fluids.  Uchenye  Zapiski Kazanskogo Universiteta. Seriya Fiziko-Matematicheskie Nauki, 2018, vol. 160, no. 1, pp. 51–60. (In Russian)

The content is available under the license Creative Commons Attribution 4.0 License.