E.N. Velichko*, E.K. Nepomnyashchaya**, E.T. Aksenov***
Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia
E-mail: *velichko-spbstu@yandex.ru, **elina.nep@gmail.com, ***et.aksenov@gmail.com
Received November 24, 2017
Abstract
Modifications of the laser correlation spectrometer system developed for the analysis of biological fluids have been discussed. Parameters of the proposed laser correlation spectrometer allowing to determine the sizes of nanoparticles in biological fluids, which are highly polydisperse solutions, to within 0.5 nm have been presented. We have explained the use of optical fiber in the spectrometer circuit as a system for collecting scattered radiation. Furthermore, we calculated the parameters of the discretization of the registered signals. It is for the correct selection of the ADC-converter. The results of the experiment showing significant differences in the size composition of donor blood serum proteins have been presented. Thus, the possibility for analyzing the composition of the blood serum of various donors and for obtaining diagnostic data has been proved.
Keywords: laser correlation spectroscopy, immune system, molecular analysis, blood serum
Acknowledgments. We are grateful to Tat'yana Aleksandrovna Bogomaz for her invaluable help during the experimental data analysis and for kindly providing us with the samples.
Figure Captions
Fig. 1. The layout of the laser correlation spectrometer: 1 – laser; 2 – focusing lens; 3 – container with the studied object; 4 – optical fiber; 5 – PMT; 6 – AD converter; 7 – computer.
Fig. 2. Data obtained as a result of the measurements of particle distribution by size in the blood serum solution of a male donor.
Fig. 3. Data obtained as a result of the measurements of particle distribution by size in the blood serum solution of a female donor.
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For citation: Velichko E.N., Nepomnyashchaya E.K., Aksenov E.T. Modification of the laser correlation spectrometer for analysis of biological fluids. Uchenye Zapiski Kazanskogo Universiteta. Seriya Fiziko-Matematicheskie Nauki, 2018, vol. 160, no. 1, pp. 51–60. (In Russian)
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