Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes

N.V. Danilova ^a*, P.A. Kuryntseva ^a**, M.Sh. Tagirov ^b***, P.Yu. Galitskaya ^a****, S.Yu. Selivanovskaya ^a*****

^aKazan Federal University, Kazan, 420008 Russia

^bTatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420059 Russia

E-mail: ^*natasha-danilova91@mail.ru, ^**polinazwerewa@yandex.ru,

^***tatniva@mail.ru, ^****gpolina33@yandex.ru, ^*****svetlana.selivanovskaya@kpfu.ru

Received March 29, 2019

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DOI: 10.26907/2542-064X.2019.3.395-407

For citation: Danilova N.V., Kuryntseva P.A., Tagirov M.Sh., Galitskaya P.Yu., Selivanovskaya S.Yu. Spreading of antibiotic resistance as a result of soil fertilization by manure composts containing oxytetracycline and antibiotic-resistant genes. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2019, vol. 161, no. 3, pp. 395–407. doi: 10.26907/2542-064X.2019.3.395-407. (In Russian)

Abstract

Due to the active usage of antibacterial drugs in animal husbandry, antibiotic residues and antibiotic-resistance genes enter the soil when it is fertilized with manure and compost. In this work, we estimated the spread of antibiotic-resistance genes in the soil when it was fertilized with composts containing oxytetracycline (OTC) and tetracycline-resistance genes. The following mixtures were studied for 35 days: the soil with “clean” compost, the soil with compost and 300 mg kg^–1 OTC, the soil with compost and antibiotic-resistance genes. In the soil treated with composts, the number of bacteria and fungi increased, with the greatest changes in the number of bacteria observed for the soil with compost containing OTC. The addition of compost with the antibiotic and the resistance genes tet(M) and tet(X) to the soil led to the formation of antibiotic resistance in soil microorganisms with the highest number of gene copies for 5–7 days: an average of 3.31?10⁶ and 7.77?10⁶ copies g^-1, respectively. Both genes, tet(M) and tet(X), but in smaller numbers, were detected in the soil with “clean” compost on days 14–21, respectively.

Keywords: antibiotics, oxytetracycline, antibiotic resistance, antibiotic-resistance genes, compost, real-time PCR

Acknowledgments. The study was supported by the Russian Science Foundation (project no. 18-29-25054).

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