Bacterial community of the brines extracted during the underground dissolution of potassium-magnesium salts of the Yakshinskoe deposit (Komi Republic, Russia)
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Bacterial community of the brines extracted during the underground dissolution of potassium-magnesium salts of the Yakshinskoe deposit (Komi Republic, Russia)

A.A. Pyankova a*, E.G. Plotnikova a**, S.N. Shanina b***

aInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, 614081 Russia

bInstitute of Geology, FRC Komi Scientific Center, Ural Branch, Russian Academy of Sciences,Syktyvkar, 167982 Russia

E-mail: *peg@iegm.ru, **annpjankva@mail.ru, ***shanina@geo.komisc.ru

Received April 5, 2022

 

ORIGINAL ARTICLE

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DOI: 10.26907/2542-064X.2022.3.457-474

For citation: Pyankova A.A., Plotnikova E.G., Shanina S.N. Bacterial community of the brines extracted during the underground dissolution of potassium-magnesium salts of the Yakshinskoe deposit (Komi Republic, Russia). Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2022, vol. 164, no. 3, pp. 457–474. doi: 10.26907/2542-064X.2022.3.457-474. (In Russian)

Abstract

This article provides the first description of the bacterial diversity in the Permian rock of the Upper Pechora salt basin. Halophilic and halotolerant bacteria of the classes Gammaproteobacteria (from the genera Halomonas, Marinobacter, and Idiomarina) and Actinomycetia (from the genus Dietzia) were isolated from the brines extracted during the underground dissolution of carnallite rocks in the pilot well of the Yakshinskoe deposit (Komi Republic, Russia) of potassium-magnesium salts. The analysis of 16S rRNA gene sequences cloned from the total DNA of the floatable part of the insoluble brine residues revealed the following bacterial classes: Gammaproteobacteria (closely related to the genera Idiomarina, Marinimicrobium, Marinobacter, Methylophaga, and Pseudidiomarina), Betaproteobacteria (phenologically similar to the genera Achromobacter and Methylotenera), as well as Alphaproteobacteria and Flavobacteriia (related to the genera Sulfitobacter and Salegentibacter, respectively). Several identified phylotypes (clones 113YS, 8YS, 150YS, and 157YS) had a low level of similarity with the closest type strains of the genera Methylophaga (M. nitratireducenticrescens JAM1T, 96.86%), Salegentibacter (S. salarius ISL-6T, 98.58%), and Methylotenera (M. versatilis 301T, 98.05% and 98.19%). This indicates that the brine samples contained new, previously undescribed, bacterial taxa. The results obtained define and shape the future prospects for studying the phylogenetic and functional diversity of microorganisms in the salt strata of the Yakshinskoe deposit.

Keywords: potassium-magnesium salt deposit, halophilic bacteria, 16S rRNA genes, cloning,    sequencing

Acknowledgements. The samples were kindly provided by the Polyarnouralgeologiya Company. The chemical composition of the brines and the insoluble residue of carnallite was determined with the help of the Institute of Geology, FRC Komi Scientific Center, Ural Branch, Russian Academy of Sciences.

The study was performed as part of state assignment no. 121112500044-9.

Figure Captions

Fig. 1. Phylogenetic tree of the studied clones based on the comparative analysis of 16S rRNA gene sequences. GenBank accession numbers are given in brackets.

Fig. 2. Taxonomic structure of the phylum Proteobacteria (a) and the class Gammaproteobacteria (b) in the floatable part of the brine residue sampled from the salt well of the Yakshinskoe deposit.

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