Generation of electricity in BFC by the biological product 'Doctor Robik' during the purification of nitrogen-containing wastewater
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Generation of electricity in BFC by the biological product 'Doctor Robik' during the purification of nitrogen-containing wastewater

G.O. Zhdanova a*, D.I. Stom a,b,c**, N.Yu. Yudina d***, S.V. Alferov d****, Z.U. Dzhangidze e*****, A.D. Stom a******, M.Yu. Tolstoy b*******,

I.A. Bogdanova a********, A.N. Chesnokova b*********

aIrkutsk State University, Irkutsk, 664003 Russia

bIrkutsk National Research Technical University, Irkutsk, 664074 Russia

cBaikal Museum, Siberian Branch, Russian Academy of Sciences, Listvyanka, Irkutsk region, 664520 Russia

dTula State University, Tula, 300012 Russia

eMoscow State University of Civil Engineering, Moscow, 129337 Russia

E-mail: *zhdanova86@ya.ru, **stomd@mail.ru, ***tysia21-05-90@mail.ru, ****chem@tsu.tula.ru, *****d_zaza@mail.ru, ******apatania@yandex.ru,

*******tolstoi@istu.edu, ********irinairk@gmail.com, *********chesnokova@istu.edu

Received February 22, 2022

 

ORIGINAL ARTICLE

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DOI: 10.26907/2542-064X.2022.2.279-296

For citation: Zhdanova G.O., Stom D.I., Yudina N.Yu., Alferov S.V., Dzhangidze Z.U., Stom A.D., Tolstoy M.Yu., Bogdanova I.A., Chesnokova A.N. Generation of electricity in BFC by the biological product “Doctor Robik” during the purification of nitrogen-containing wastewater. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2022, vol. 164, no. 2, pp. 279–296. doi: 10.26907/2542-064X.2022.2.279-296. (In Russian)

Abstract

In this study, we evaluated the efficiency of a biofuel cell technology (BFC) based on the commercial biological product “Doctor Robik 109” in the utilization of some nitrogen-containing components of wastewater (urea, ammonium nitrogen, and peptone). The following electrochemical parameters were measured during the BFC operation with “Doctor Robik 109”: voltage, current strength, power, and redox potential of the anolyte. The nitrogen-containing substrates were determined using photometric methods. The number of microorganisms was calculated by Koch’s method. It was shown that “Doctor Robik 109” generated an electric current in the BFC upon the usage of urea (0.5 g/L), ammonium nitrogen (0.5 g/L of NH4+ ions), and peptone (0.5 g/L) as substrates. The current generation by microorganisms of “Doctor Robik 109” in the BFC was accompanied by an increase in the number of cells of microorganisms, a depletion of the tested substrates, and a decrease in the redox potential of the anolyte. In the BFC containing model wastewater with the addition of 0.5 g/L of urea, “Doctor Robik 109” generated an open-circuit voltage of up to 332 mV (for 5 days). With 0.5 g/L NH4+ and 0.5 g/L peptone, this value was higher – up to 483 mV (for 1 day) and up to 520 mV (for 10 days), respectively. The current strength (in the short-circuit mode) in the BFC with urea reached 519 μA, and in the BFC with peptone it was more than two times higher (up to 1248 μA). Based on the data obtained, with an account of the breadth of the substrate specificity of microorganisms of “Doctor Robik 109”, its low cost and ease of use, recommendations were given for the use of this biological product in BFC for waste disposal and treatment of various types of wastewater with simultaneous generation of electric current.

Keywords: biofuel cells, generation of electric current, bioagent microorganisms, biological products, “Doctor Robik”, nitrogen-containing wastewater, wastewater treatment

Acknowledgments. This study was supported by the Ministry of Science and Higher Education of the Russian Federation as part of the state assignment in the field of research (project no. FEWG-2021-0013).

Figure Captions

Fig. 1. Dynamics of voltage (a) and current strength (b) generated in the BFC by the microbial preparation “Doctor Robik 109” when using urea (medium – model wastewater, electrodes – carbon cloth, urea concentration – 0.5 g/L).

Fig. 2. Dynamics of the number of viable cells of the microbial preparation “Doctor Robik 109” and the concentration of urea in the BFC anolyte during its operation (medium – model wastewater, electrodes – carbon cloth, urea concentration - 0.5 g/L).

Fig. 3. Dynamics of voltage (a) and change in the concentration of ammonium nitrogen (b) in the BFC under the action of the microbial preparation “Doctor Robik 109” (medium – model wastewater, initial content of NH4+ – 0.5 g/L (in the form of NH4Cl), electrodes – carbon cloth).

Fig. 4. Dynamics of voltage (a) and current strength (b) generated in the BFC by the microbial preparation “Doctor Robik 109” when using peptone (medium – model wastewater, electrodes – carbon cloth, peptone concentration – 0.5 g/L).

Fig. 5. Dynamics of the redox potential of the BFC anolyte containing the native and inactivated microbial preparation “Doctor Robik 109” as a bioagent (medium – model wastewater, electrodes – carbon cloth, peptone concentration – 0.5 g/L).

Fig. 6. Changes in the indicator of chemical oxygen consumption of the anolyte of the studied BFCs (native and autoclaved preparation “Doctor Robik 109”, medium – model wastewater, substrate – peptone 0.5 g/L).

Fig. 7. Dependence of the power of the BFC with the microbial preparation “Doctor Robik 109” on external resistance (medium – model wastewater, substrate – peptone 0.5 g/L, electrodes – carbon cloth, BFC exposure time – 2 days).

Fig. 8. Dependence of the power of the BFC with the microbial preparation “Doctor Robik 109” for two days of exposure, on external resistance (medium – model wastewater, substrate – sodium acetate 0.5 g/L + urea 0.5 g/L, electrodes – carbon cloth).

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