Effect of mycotoxins on the ultrastructure of cortical elements in pig nephrons

N.V. Shakurova ^a*, E.I. Semenov ^b**, V.B. Savva ^c***

^aKazan Federal University, Kazan, 420008 Russia

^bFederal Center for Toxicological, Radiation, and Biological Safety, Kazan, 420075 Russia

^cKazan National Research Technological University, Kazan, 420015 Russia

E-mail: ^*ntlshakurova@gmail.com, ^**semyonovei@bk.ru, ^***Victoria511@mail.ru

Received July 15, 2019

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DOI: 10.26907/2542-064X.2020.3.350-360

For citation: Shakurova N.V., Semenov E.I., Savva V.B. Effect of mycotoxins on the ultrastructure of cortical elements in pig nephrons. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2020, vol. 162, no. 3, pp. 350–360. doi: 10.26907/2542-064X.2020.3.350-360. (In Russian)

Abstract

This paper reports the results of an electron microscopic study of pathomorphological changes in the kidneys of pigs exposed to the combined intoxication with fusariotoxins (T-2 toxin, deoxynivalenol, and zearalenone). Mycotoxicosis-related cytological changes of the glomeruli and proximal convoluted tubules were analyzed. A series of ultrastructural changes that occurred in the cellular elements involved in ultrafiltration and reabsorption were observed and ascribed to the development of nephropathy under chronic mycotoxication.

Keywords: mycotoxin, nephron, ultrastructure, fusariotoxins, T-2 toxin, deoxynivalenol, zearalenone, electron microscopy

Figure Captions

Fig. 1. Chromato-mass spectrum of T-2 toxin (Fermentek ltd., Israel).

Fig. 2. Chromato-mass spectrum of T-2 toxin (FSBSI “FCTRBS-RRVI”, Russia).

Fig. 3. Renal corpuscle. Capillary network encircled with podocytes: a – combined intoxication with fusariotoxins, b – control (norm). On the right part of the images – a fragment of the capillary glomerulus. Endothelial plasmalemma forms villous outgrowths facing the vessel lumen. BM – trilaminar basal membrane separating the vessel from podocytes of the capsule. MT – mitochondria, ER – endoplasmic reticulum. Scale bar: 1 µm.

Fig. 4. Renal corpuscle (norm). Slit diaphragm formed by the cytopedicles of podocytes and the trilaminar basal membrane (BM). Scale bar: 1 µm.

Fig. 5. Proximal tubule. Apical surface of the epitheliocytes: a – combined intoxication with fusariotoxins, b – control (norm). Bush-border tubules localized to the base of the microvilli (MV). Scale bar: 1 µm.

Fig. 6. Proximal tubule. Basal labyrinth: a – combined intoxication with fusariotoxins, b – control (norm). Abbreviations: N – epitheliocyte nucleus, BL – basal labyrinth, BM – basal membrane. Scale bar: 1 µm.

References

1. Food and Health in Europe: A New Basis for Action. Robertson A., Tirado C., Lobstein T., Jermini M., Knai C., Jensen J.H., Ferro-Luzzi A., James W.P.T. (Eds.). 2004, xvi + 388 p. WHO Regional Publications, European Series, no. 96.
2. Gereza J.R., Pinton P., Callud P.¸ Grosjeand F., Oswaldb I.P., Bracarensea A.P. Deoxynivalenol alone or in combination with nivalenol and zearalenone induce systemic histological changes in pigs. Exp. Toxicol. Pathol., 2015, vol. 67, no. 2, pp. 89–98. doi: 10.1016/j.etp.2014.10.001.
3. Semenov E.I., Tremasov M.Y., Matrosova L.E., Tarasova E.Y., Kryuchkova M.A., Smolentsev S.Y., Korosteleva V.P. Joint effect of the mycotoxins T-2 toxin, deoxynivalenol and zearalenone on the weaner pigs against a background of the infection load. Res. J. Pharm., Biol. Chem. Sci., 2016, vol. 7, no. 1, pp. 1860–1868.
4. Antonissen G., Martel A., Pasmans F., Ducatelle R., Verbrugghe E., Vandenbroucke V., Li Sh., Haesebrouck F., Van Immerseel F., Croubels S. The impact of Fusarium mycotoxins on human and animal host susceptibility to infectious diseases. Toxins (Basel), 2014, vol. 6, no. 2, pp. 430–452. doi: 10.3390/toxins6020430.
5. Ivanov A.V., Fisinin V.I., Tremasov M.Ya., Papunidi K.Kh. Mikotoksikozy (biologicheskie i veterinarnye aspekty) [Mycotoxicoses (Biological and Veterinary Aspects)]. Moscow, Kolos, 2010. 392p. (In Russian)
6. Meky F.A., Hardie L.J., Evans S.W., Wild C.P. Deoxynivalenol-induced immunomodulation of human lymphocyte proliferation and cytokine production. Food Chem. Toxicol., 2001, vol. 39, no. 8, pp. 827–836. doi: 10.1016/S0278-6915(01)00029-1.
7. Mowaffaq A.A.A., Yasser M.T., Khirun B.M., Doblin A.S. Effects of different mycotoxins on humans, cell genome and their involvement in cancer (Review). Oncol. Rep., 2017, vol. 37, no. 3, pp. 1321–1336. doi: 10.3892/or.2017.5424.
8. Vidal D.R. Propriétés immunosuppressives des mycotoxines du groupe des trichothecenes. Bull. Inst. Pasteur, 1990, vol. 88, no. 2, pp. 159–192. (In French)
9. Smith T.K., MacDonald E.J., Haladi S. Current concepts in feed-borne mycotoxins and the potential for dietary prevention of mycotoxicoses. Science and Technology in the Feed Industry: Proc. Alltech’s 17th Annu. Symp. Lyons T.P., Jacques K.A. (Eds.). Nottingham, UK, Nottingham Univ. Press, 2001, pp. 183–190.
10. Basso K., Gomes F., Loureiro Bracarense A.P. Deoxynivanelol and fumonisin, alone or in combination, induce changes on intestinal junction complexes and in E-cadherin expression. Toxins (Basel), 2013, vol. 5, no. 12, pp. 2341–2352. doi: 10.3390/toxins5122341.
11. Salnikova M.M., Idiyatov I.I., Saitov V.R. Brain cortex ultrastructure in pigs exposed to dioxin, T-2 toxin, and therapeutic drugs. Vet. Vrach, 2015, no. 5, pp. 3–8. (In Russian)
12. Saitov V.R., Ivanov A.V., Salnikova M.M. Hepatocyte ultrastructure in pigs exposed to dioxin, T-2 toxin, and therapeutic drugs. Vet. Vrach, 2013, no. 6, pp. 2–5. (In Russian)
13. Osyanin K.A., Papunidi K.Kh., Saitov V.R., Salnikova M.M. Morphometry of white pulp mitochondria in pigs and rabbits after intoxication and treatment with therapeutic drugs. Uch. Zap. Kazan. Gos. Akad. Vet. Med. im. N.E. Baumana, 2014, no. 217, pp. 200–205. (In Russian)
14. Saitov V.R., Salnikova M.M., Kadikov I.R. Cardiomyocyte ultrastructure in sheep exposed to dioxin and T-2 toxin. Mater. V Mezhdunar. nauch.-prakt. konf. posvyashch. 85-letiyu Gorno-Altaiskogo nauch.-issled. inst. sel’sk. khoz. “Aktual’nye problemy sel’skogo khozyaistva gornykh territorii” [Proc. V Int. Sci. Pract. Conf. Celebrating the 85th Anniversary of Gorno-Altaysk Research Institute of Agriculture “Urgent Problems of Agriculture in Mountainous Areas”], 2015, pp. 123–129. (In Russian)
15. Sergeichev A.I. Providing reference samples to laboratories for mycotoxin studies. Mater. mezhdunar. simp. “Nauchnye osnovy obespecheniya zashchity zhivotnykh ot ekotoksikantov, radionuklidov i vozbuditelei opasnykh infektsionnykh zabolevanii”, 28–30 noyab., 2005 g. [Proc. Int. Symp. “Scientific Basis for the Protection of Animals from Ecotoxicants, Radionuclides, and Agents of Dangerous Infections”, Nov. 28–30, 2005”]. Pt. 1. Kazan, 2005, pp. 240–243. (In Russian)
16. Letter of Instruction 434-17. On the MRL of mycotoxins in fodder. USSR General Directorate for Veterinary, 1989. (In Russian)
17. Metodicheskie rekomendatsii po diagnostike, profilaktike i lecheniyu mikotoksikozov zhivotnykh [Guidelines for Diagnosis, Prevention, and Treatment of Animal Mycotoxicoses]. Moscow, FGBNU “Rosinfarmagrotekh”, 2017. 68 p. (In Russian)
18. Obremski K., Zielonka Ł., Gajęcka M., Jakimiuk E., Bakuła T., Baranowski M., Gajęcki M. Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol, T-2 toxin and zearalenone in the pig. Pol. J. Vet. Sci., 2008, vol. 11, no. 4, pp. 339–345.
19. Labuda R., Parich A., Berthiller F., Tančinová D. Incidence of trichothecenes and zearalenone in poultry feed mixtures from Slovakia. Int. J. Food Microbiol., 2005, vol. 105, no. 1, pp. 19–25. doi: 10.1016/j.ijfoodmicro.2005.06.005.
20. Jansen A., Cook T., Taylor M., Largen P., Riveros-Moreno V., Moncada S., Cattell V. Induction of nitric oxide synthase in rat immune complex glomerulonephritis. Kidney Int., 1994, vol. 45, no. 4, pp. 1215–1219. doi: 10.1038/ki.1994.161.

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