Bioaffinity-based method for catecholamines determination with amperometric DNA sensor

S.S. Babkina ^a*, N.A. Ulakhovich ^b**, E.P. Medyantseva ^b***, A.R. Gataulina ^b****

^aMIREA – Russian Technological University, Moscow, 119454 Russia

^bKazan Federal University, Kazan, 420008 Russia

E-mail: ^*sofya.babkina@gmail.com, ^**Nikolay.Ulakhovich@kpfu.ru, ^***emedyant@gmail.com, ^****alphiag@mail.ru

Received October 23, 2019

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DOI: 10.26907/2542-064X.2020.1.5-16

For citation: Babkina S.S., Ulakhovich N.А., Medyantseva E.P., Gataulina A.R. Bioaffinity-based method for catecholamines determination with amperometric DNA sensor. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2020, vol. 162, no. 1, pp. 5–16. doi: 10.26907/2542-064X.2020.1.5-16. (In Russian)

Abstract

A bioaffinity-based method for the determination of adrenaline and dopamine involving immobilized renatured DNA incorporated into the amperometric biosensor was developed.

With the help of spectrophometry and voltammetry data, it was found that the composition of complexes formed with catecholamines and their stability constants were changing with concentration from [КАL₂] to [КА₂L], where КА – catecholamine, L – base pare, and lg β_eff = 10.1–13.5. The high affinity of adrenaline and dopamine to the immobilized renatured DNA molecule was revealed, thereby making it possible to run effective concentration of KA from the analyzed solutions of low concentration including multicomponent fluids.

The minimum reporting level was found to be 5.5·10^–9 (adrenaline) and 2.0·10^–8 (dopamin) mol/L, respectively. The proposed method is characterized by high sensitivity, reproducibility (relative accuracy does not exceed 5%), and single determination time shorter than 30 min.

Keywords: adrenaline, amperometric sensor, bioaffinity-based method, biosensor, DNA, dopamine, catecholamines

Figure Captions

Fig. 1. Changes in the mole fraction of adrenaline – r-IDNA complexes: 1 – changes in the mole fraction of the [AD₂L] complex; 2 – changes in the mole fraction of the [ADL₂] complex; c_DNA = 0.01 mg/mL, acetate buffer, pH 4.9.

Fig. 2. Changes in the mole fraction of dopamine – r-IDNA complexes: 1 – changes in the mole fraction of the [DA₂L] complex; 2 – changes in the mole fraction of the [DАL₂] complex; c_DNA = 0.01 mg/mL, acetate buffer, pH 4.9.

Fig. 3. Dependence of the oxidation current of AD and DA on the preconcentration time of catecholamines on the biosensor containing r-IDNA: 1 – c_AD = 1·10^–7 mol/L; 2 – c_DA = 5·10^–6 mol/L.

Fig. 4. Dependence of the oxidation current of AD and DA on the reactivation time of the biosensor containing r-IDNK: 1 – c_AD = 1·10^–7 mol/L; 2 – c_DA = 5·10^–6 mol/L.

References

1. Mashkovskii M.D. Lekarstvennye sredstva [Medicinal Drugs]. Vol. 1. Moscow, Novaya Volna, 2002. 539 p. (In Russian)
2. Chissov V.I., Dar’yalova S.L. Onkologiya [Oncology]. Moscow, GEOTAR Med., 2007. 560 p. (In Russian)
3. Paleček E., Bartošík M. Intrinsic electrocatalysis in DNA. ChemElectroChem, 2017, vol. 5, no. 6, pp. 936–942. doi: 10.1002/celc.201700888.
4. Čbarkapa A., Živković L., Žuvkoetc D., Djelić N., Bajić V., Dekanski D., Spremo-Potparević B. Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. Toxicol. in Vitro, 2014, vol. 27, no. 3, pp. 451–456. doi: 10.1016/j.tiv.2013.12.014.
5. Yang G.-J., Xu J.-J., Chen H.-Y. Studies on the interaction between catecholamine derivatives and DNA by means of spectroscopic and voltammetric methods. Chem. J. Chin. Univ., 2004, vol. 25, no. 7, pp. 1235–1239.
6. Zheng S.-J., Lin X.-Q. Spectral and electrochemical investigation of intercalations of adrenaline and CT-DNA. Chin. J. Chem., 2003, vol. 21, no. 7. pp. 767–771. doi: 10.1002/cjoc.20030210712.
7. Veselova I.A., Sergeeva E.A., Makedonskaya M.I., Eremina O.E., Kalmykov S.N., Shekhovtsova T.N. Methods for determining neurotransmitter metabolism markers for clinical diagnostics. J. Anal. Chem., 2016, vol. 71, no. 12, pp. 1155–1168. doi: 10.7868/S0044450216120124.
8. Babkina S.S. Nanoanalytics: Application of sensors based on the bioaffinne interaction in medicine, pharmacology, and ecology analysis. Nanotekhnol.: Nauka Proizvod., 2009, no. 2, pp. 23–27. (In Russian)
9. Babkina S.S., Budnikov Н.С. Electrochemical biosensors based on nucleic acids and their use in bioaffinity assays for determining DNA and its effectors. J. Anal. Chem., 2006, vol. 61, no. 8, pp. 728–739. doi: 10.1134/S1061934806080028.
10. Babkina S.S., Moiseeva E.N., Ulakhovich N.A. Doxorubicin determination by means of an amperometric biosensor. Farmatsiya, 2006, no. 5, pp. 9–11. (In Russian)
11. Budnikov H.C., Medyantseva E.P., Babkina S.S. An enzyme amperometric sensor for toxicants determination. J. Electroanal. Chem., 1991, vol. 310, nos. 1–2, pp. 49–55. doi: 10.1016/0022-0728(91)85251-J.
12. Shcherbakova É.S., Gol’dshtein I.P., Gur’yanova E.N., Kocheshkov K.A. Method of treatment of the results of a physicochemical investigation of complex compounds in solutions on an electronic computer. Bull. Acad. Sci. USSR, Div. Chem. Sci., 1975, vol. 24, no. 6, pp. 1165–1172. doi: 10.1007/BF00922039.
13. Salnikov Yu.I., Glebov A.N., Devyatov F.V. Poliyadernye kompleksy v rastvorakh [Polynuclear Complexes in Solutions]. Kazan, Izd. Kazan. Gos. Univ., 1989. 288 p. (In Russian)
14. Babkina S.S., Vinter V.G., Zainullina A.S. Immunoenzyme method for the determination of low-molecular organic-compounds with spectrophotometric indication of analytical signals. J. Anal. Chem., 1994, vol. 49, no. 10, pp. 1005–1010.
15. Rauf S., Gooding J.J., Ahtar K. Electrochemical approach of anticancer drugs-DNA interaction. J. Pharm. Biomed. Anal., 2005, vol. 37, no. 2, pp. 205–217. doi: 10.1016/j.jpba.2004.10.037.
16. Piedade J.A.P., Fernandes I.R., Oliveira-Brett A.M. Electrochemical sensing of DNA-adriamycin interactions. Bioelectrochemistry, 2002, vol. 56, nos. 1–2, pp. 81–83. doi: 10.1016/S1567-5394(02)00013-0.

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