Phenolic plant defense system under conditions of environment pollution by heavy metals in Tyumen

A.S. Petukhov^*, N.A. Khritokhin^**, G.A. Petukhova^***, T.A. Kremleva^****

University of Tyumen, Tyumen, 625003 Russia

E-mail: ^*revo251@mail.ru, ^**kna@utmn.ru, ^***gpetuhova1@mail.ru, ^****kreml-ta@yandex.ru

Received October 18, 2018

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DOI: 10.26907/2542-064X.2019.1.93-107

For citation: Petukhov A.S., Khritokhin N.A., Petukhova G.A., Kremleva T.A. Phenolic plant defense system under conditions of environment pollution by heavy metals in Tyumen. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2019, vol. 161, no. 1, pp. 93–107. doi: 10.26907/2542-064X.2019.1.93-107. (In Russian)

Abstract

The mechanisms of plant persistence to the heavy metals effect, including the change in flavonoids and phenolic antioxidants status, is of great importance. The aim of this research is to investigate the impact of heavy metal (Cu, Fe, Mn, Pb, and Cd) accumulation in herbs of various species in Tyumen on the content of phenols and flavonoids.

The material has been collected near various industrial facilities of Tyumen: metallurgical, engine-building, oil refinery, and battery. The studied sites also include the highway area. The following species of plants have been sampled: coltsfoot (Tussilago farfara), meadow grass (Poa pratensis), red clover (Trifolium rubens), chamomile (Matricaria chamomilla), and wild vetch (Vicia cracca). The analysis of heavy metal concentrations in plants has been performed by atomic absorption spectrophotometry. The concentration of phenols in plants has been determined by titration, while the analysis for flavonoids has been carried out photometrically.

The species-specific accumulation of heavy metals has been observed. It has been found that the highest content of Mn is accumulated by coltsfoot. In general, the accumulation of heavy metals decreases in the following order: Fe > Cu > Mn > Pb > Cd. The highest concentrations of heavy metals were registered near the metallurgical plant. The change in the status of phenols and flavonoids has also turned out to be species-specific. In the cells of coltsfoot, the content of phenols and flavonoids is higher than in the control plants almost in all test groups. At the same time, the concentration of antioxidants in red clover decreases. The high correlation coefficients have been registered for the Cu, Fe, and Mn concentration and the content of phenols and flavonoids, which proves the impact of heavy metals on the antioxidant defense system of plants under conditions of the urban environment.

Keywords: heavy metals, phenols, flavonoids, antioxidants, plants, urban environment

References

1. Kabbata-Pendias A, Pendias H. Trace Elements in Soils and Plants. CRC Press Inc., 1984. 315 p.

2. Titov A.F., Kaznina N.M., Talanova V.V. Tyazhelye metally i rasteniya [Heavy Metals and Plants]. Petrozavodsk, Karel. Nauchn. Tsentr Ross. Akad. Nauk, 2014. 194 p. (In Russian)

3. Hassanein R.A., Hashem H.A., El-Deep M.H., Shouman A. Soil contamination with heavy metals and its effect on growth, yield and physiological responses of vegetable crop plants (turnip and lettuce). J. Stress Physiol. Biochem., 2013, vol. 9, no. 4, pp. 145–162.

4. Skugoreva S.G., Ashikhmina T.Ya., Fokina A.I., Lyalina E.I. Chemical grounds of toxic effect of heavy metals (A review). Teor. Prikl. Ekol., 2016, no. 1, pp. 1–10. (In Russian)

5. Skorzynska-Polit E. Lipid peroxidation on plant cells, its physiological role and changes under heavy metal stress. Acta Soc. Bot. Pol., 2007, no. 76, no. 1, pp. 49–54. doi: 10.5586/asbp.2007.006.

6. Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol. J. Environ. Stud., 2006, vol. 15, no. 4, pp. 523–530.

7. Mierziak J, Kostyn K, Kulma A. Flavonoids as important molecules of plant interaction with the environment. Molecules, 2014, vol. 19, no. 10, pp. 16240–16265. doi: 10.3390/molecules191016240.

8. Vysochina G.I., Kukushkina T.A., Kotsupii O.V., Zagurskaya Yu.V., Bayandina I.I. Flora of the forest-steppe zone of West Siberia as a source of biologically active compounds. Contemp. Probl. Ecol., 2011, vol. 4, no. 2, pp. 202–211. doi: 10.1134/S1995425511020123.

9. Rakhmankulova Z.F., Shuyskaya E.V., Shcherbakov A.V., Fedyaev V.V., Biktimerova G.Ya., Kha­fisova R.R., Usmanov I.Yu. Content of proline and flavonoids in the shoots of halophytes inhabiting the South Urals. Russ. J. Plant Physiol., 2015, vol. 62, no. 1, pp. 71–79. doi: 10.1134/S1021443715010112.

10. Velikanova N.A., Slivkin A.I., Gaponov S.P. Investigation of flavonoids accumulation by knotgrass collected from ecologically different areas of Voronezh and its outskirts. Vestn. Voronezh. Gos. Univ. Ser.: Khim. Biol. Farm., 2013, no. 1, pp. 181–185. (In Russian)

11. Karpova E.A., Khramova E.P. Phenolic composition and content of representatives of the genus Spiraea L. under the conditions of industrial pollution in Novosibirsk. Contemp. Probl. Ecol., 2014, vol. 7, no. 2, pp. 228–236. doi: 10.1134/S1995425514020073.

12. Kulbat K., Leszczynska J. Antioxidants as defensive shield in thyme (Thymus vulgaris L.) grown on the soil contaminated with heavy metals. Biotechnol. Food Sci., 2016, vol. 80, no. 2, pp. 109–117.

13. Marquez-Garcia B., Angeles-Fernandez-Recamales M., Cordoba F. Effects of cadmium on phenolic composition and antioxidant activities of Erica andevalensis. J. Bot., 2012, vol. 2012, art. 936950, pp. 1–6. doi: 10.1155/2012/936950.

14. Lachman J., Dudjak J., Miholova D., Kolihova D., Pivec V. Effect of cadmium on flavonoid content in young barley (Hordeum sativum L.) plants. Plant, Soil Environ., 2005, vol. 51, no. 11, pp. 513–516. doi: 10.17221/3625-pse.

15. Metodika opredeleniya sodrzhaniya tyazhelykh metallov v zole rastenii [Method for Heavy Metal Content Determination in Plant Ashes]. Moscow, Vyssh. Shk., 1990. 32 p. (In Russian)

16. General Pharmacopoeial Standard 1.5.3.0008.15. Determination of tannins in medicinal herbal raw materials and herbal medicine. Moscow, 2015, 4 p. (In Russian)

17. Ermakov A.I., Arasimovich V.V., Yarosh N.P., Peruanskii Yu.V., Lukovnikova G.A., Ikonnikova M.I. Metody biokhimicheskogo issledovaniya rastenii [Methods for Biochemical Study of Plants]. Leningrad, Agropromizdat, 1987, 430 p. (In Russian)

18. Kopylova L.V. Accumulation of Fe and Mn in the leaves of arboreous plants from technogenic areas of the Trans-Baikal region. Izv. Samar. Nauchn. Tsentra Ross. Akad. Nauk, 2010, vol. 12, no. 1, pp. 709–712. (In Russian)

19. Voskresenskaya O.L., Voskresenskii V.S., Alyabysheva E.A. Heavy metal accumulation by soil and plants in the locations of gathering and temporary storage of solid waste. Sovrem. Probl. Obraz., 2013, no. 2, pp. 1–8. Available at: https://www.science-education.ru/pdf/2013/2/112.pdf. (In Russian)

20. Blokhina O., Virolainen E., Fagerstedt K.V. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann. Bot., 2002, vol. 91, no. 2, pp. 179–194. doi: 10.1093/aob/mcf118.

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