Русский
  • Choose language
    • Русский
    • Español
    • 中文
Main page \ Research and Innovations \ Publications \ Publishing activities of KFU \ Uchenye Zapiski Kazanskogo Universiteta \ Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki \ Archive

Cytotoxic and Apoptosis-Inducing Activity of Plants from the Family Asparagaceae in Relation to Human Alveolar Adenocarcinoma Cells

Y.N. Kamalova a*, V.V. Shtyreva a, Essam Abdul-Hafeez b**, Omer H.M. Ibrahim b***, P.V. Zelenikhin a****, N.S. Karamova a*****, O.N. Ilinskaya a******

a Kazan Federal University, Kazan, 420008 Russia

b Assiut University, Assiut 71515 Arab Republic of Egypt

E-mail: *yazgulen@mail.ru, **noresam_2000@yahoo.com, ***omer_hooo@yahoo.com, ****pasha_mic@mail.ru, *****nskaramova@mail.ru, ******ilinskaya_kfu@mail.ru

Full text PDF

Abstract

Cancer is known as the second major mortality cause. The number of new cases is increasing every year. Thus, it is urgent for scientists to search for alternative drugs with selective antitumor action and minimal side effects. It is known that some plant metabolites exhibit antioxidant, cytotoxic, and antitumor activity, while at the same time being less toxic than modern allopathic drugs. In this work, we have investigated the cytotoxic and apoptosis-inducing effects of extracts obtained from plants of the family Asparagaceae on A549 human lung adenocarcinoma cells. The analysis has been performed using flow cytofluorometry. If extracts showed cytotoxicity, the apoptosis-inducing action has been evaluated at the concentration of 50 μg/mL; in other cases, the analyzed concentration range was 50–300 μg/mL. On the basis of the experiments carried out, the following conclusions have been made. Extracts of the leaves and rhizomes of Sansevieria cylindrica and Sansevieria trifasciata do not possess antitumor activity. Extracts of the leaves of Polianthes tuberosa and Furcraea gigantea, which were cytotoxic at high concentrations, cause cell death at 50 μg/mL in the amount of 21.35 ? 1.86 and 15.6 ? 3.23, respectively. Extracts of Polianthes tuberosa bulbs and Yucca filamentosa leaves are able to induce apoptosis at higher concentrations. When the concentration reaches 100 μg/mL, the proportion of apoptotic cells for these plants is 45.76 ? 1.34 and 11.33 ? 0.07, respectively. The number of dead cells at the concentration of 300 μg/mL increased up to 73.33 ? 3.05 and 81.75 ? 4.07. The results have great importance for development of new drugs based on metabolites from these plant extracts.

Keywords: cancer, apoptosis, plant extracts, Asparagaceae

Acknowledgments. This study was supported by the state program for increasing the competitiveness of Kazan Federal University, Russian Foundation for Basic Research (project no. 15-54-61024), and Science Technology Development Fund (STDF) of Egypt (project no. 13821).

Figure Captions

Fig. 1. Apoptosis-inducing effect of plant extracts. Differences from control significant at р ≤ 0.05 are marked with asterisks.

References

  1. Sawadogo W.R., Schumacher M., Teiten M-H., Dicato M., Diederich M. Traditional West African pharmacopeia, plants and derived compounds for cancer therapy. Biochem. Pharmacol., 2012, vol. 84, no. 10, pp. 1225–1240. doi: 10.1016/j.bcp.2012.07.021.
  2. Mbaveng A.T., Kuete V., Mapunya B.M., Beng V.P., Nkengfack A.E., Meyer J.J.M., Lall N.  Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse trans­criptase activities. Environ. Toxicol. Pharmacol., 2011, vol. 32, no. 2, pp. 162–167. doi: 10.1016/j.etap.2011.04.006.
  3. Unnati S., Ripal S., Sanjeev A., Niyati A. Novel anticancer agents from plant sources. Chin. J. Nat. Med., 2013, vol. 11, no. 1, pp. 16−23. doi: 10.1016/S1875-5364(13)60002-3.
  4. Alonso-Castro A.J., Villarreal M.L., Salazar-Olivo L.A., Gomez-Sanchez M., Dominguez F., Garcia-Carranca A. Mexican medicinal plants used for cancer treatment: pharmacological, phytochemical and ethnobotanical studies. J. Ethnopharmacol., 2011, vol. 133, no. 3, pp. 945−972. doi: 10.1016/j.jep.2010.11.055.
  5. Cabrera-Fuentes H.A., Aslam M, Saffarzadeh M, Kolpakov A.I., Zelenikhin P.V., Preissner K.T., Ilinskaya O.N. Internalization of Bacillus intermedius ribonuclease (BINASE) induces human     alveolar adenocarcinoma cell death. Toxicon, 2013, vol. 69, no. 3, pp. 219−226. doi: 10.1016/j.toxicon.2013.03.015.
  6. Khazir J., Mir B.A., Pilcher L., Riley D.L. Role of plants in anticancer drug discovery. Phytochem. Lett., 2014, vol. 7, pp. 173–181. doi:10.1016/j.phytol.2013.11.010.
  7. Tagne R.S., Telefo B.P., Nyemb J.N., Yemele D.M., Njina S.N., Goka S.M., Lienou L.L., Nwabo Kamdje A.H., Moundipa P.F., Farooq A.D. Anticancer and antioxidant activities of methanol extracts and fractions of some Cameroonian medicinal plants. Asian Pac. J. Trop. Med., 2014, vol. 7, suppl. 1, pp. S442–S447. doi: 10.1016/S1995-7645(14)60272-8.
  8. Karamova N.S., Fatykhova D.G., Abdrakhimova J.R., Ilinskaya O.N. An investigation of antigenotoxic properties of plant extracts of Chelidonium majus L., Plantago major L. and Tussilago farfara L. Russ. J. Genet.: Appl. Res., 2011, vol. 1, no. 5, pp. 371–378. doi:10.1134/S207905971105008X.
  9. Abdul-Hafeez E.Y., Karamova N.S., Ilinskaya O.N. Antioxidant activity and total phenolic compounds content of certain medicinal plants. Int. J. Biosci., 2014, vol. 5, no. 9, pp. 213–222. doi: 10.12692/ijb/5.9.213-222.
  10. Abdul-Hafeez E.Y, Nguyen T.N., Karamova N.S., Ilinskaya O.N. Antibacterial activity of certain medicinal plants on different bacterial strains associated with colorectral cancer. Int. J. Biosci., 2014, vol. 5, no. 7, pp. 219–229. doi 10.12692/ijb/5.7.219-229.
  11. El-Seedi H.R., Burman R., Mansour A., Turki Z., Boulos L., Gullbo J., Göransson U. The traditional medical uses and cytotoxic activities of sixty-one Egyptian plants: discovery of an active cardiac glycoside from Urginea maritime. J. Ethnopharmacol., 2013, vol. 145, no. 3, pp. 746–757. doi: 10.1016/j.jep.2012.12.007.
  12. Freshney R. I. Culture of Animal Cells. A Manual of Basic Techniques. New York, Wiley-Liss, 1994, 3 ed. 486 p. doi: 10.1002/cbf.646.
  13. Smolewski P., Grabarek J., Lee B.W., Johnson G.L., Darzynkiewicz Z. Kinetics of HL-60 cell entry to apoptosis during treatment with TNF-alpha or camptothecin assayed by the stathmo-apoptosis method. Cytometry, 2002, vol. 47, no. 3, pp. 143–149.
  14. Rezk A., Al-Hashimi A., John W., Schepker H., Ullrich M.S., Brix K. Assessment of cytotoxicity exerted by leaf extracts from plants of the genus Rhododendron towards epidermal keratinocytes and intestine epithelial cells. BMC Complementary Altern. Med., 2015, vol. 15, art. 364, pp. 1–18. doi: 10.1186/s12906-015-0860-8.
  15. Lizard G., Monier S., Cordelet C., Gesquière L., Deckert V., Gueldry S., Lagrost L., Gambert P. Characterization and comparison of the mode of cell death, apoptosis versus necrosis, induced by 7b-hydroxycholesterol and 7-ketocholesterol in the cells of the vascular wall. Arterioscler., Thromb., Vasc. Biol., 1999, vol. 19, no. 5, pp. 1190–1200.
  16. Laakko T., King L., Fraker P. Versatility of merocyanine 540 for the flow cytometric detection of apoptosis in human and murine cells. J. Immunol. Methods, 2002, vol. 261, nos. 1–2, pp. 129–139.
  17. Fabricant D.S., Farnsworth N.R. The value of plants used in traditional medicine for drug discovery. Environ. Health Perspect., 2001, vol. 109, suppl. 5, pp. 69–75.
  18. Adeyemi O.O., Akindele A.J., Ogunleye E.A. Evaluation of the antidiarrhoeal effect of Sansevieria liberica Gerome & Labroy (Agavaceae) root extract. J. Ethnopharmacol., 2009, vol. 123, no. 3, pp. 459–463. doi: 10.1016/j.jep.2009.03.023.
  19. Amida M.B., Yemitan O.K., Adeyemi O.O. Toxicological assessment of the aqueous root extract of Sansevieria liberica Gerome and Labroy (Agavaceae). J. Ethnopharmacol., 2007, vol. 113, no. 1, pp. 171–175.
  20. Akindele A.J., Wani Z.A., Sharma S., Mahajan G., Satti N.K., Adeyemi O.O., Mondhe D.M., Saxena A.K. In vitro and in vivo anticancer activity of root extracts of Sansevieria liberica Gerome and Labroy (Agavaceae). J. Evidence-Based Complementary Altern. Med., 2015, vol. 2015, art. ID 560404, pp. 1–11. doi:10.1155/ 2015/560404.
  21. Roy J., Kuddus M., Begum B., Choudhury H. Evaluation of analgesic, cytotoxic and antioxidant activities of Sansevieria roxburghiana Schult. and Schult. f. Asian Pac. J. Trop. Med., 2012, vol. 2, no. 2, pp. S723–S726. doi:10.1016/S2221-1691(12)60303-7.
  22. Haldar P.K., Kar B., Bala A., Bhattacharya S., Mazumder U.K. Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharm. Biol., 2010, vol. 48, no. 12, pp. 1337–1343. doi: 10.3109/13880201003792592.
  23. Balestrieri C., Felice F., Piacente S., Pizza C., Montoro P., Oleszek W., Visciano V., Balestrieri M.L. Relative effects of phenolic constituents from Yucca schidigera Roezl. bark on Kaposi's sarcoma cell proliferation, migration, and PAF synthesis. Biochem. Pharmacol., 2006, vol. 71, no. 10, pp. 1479–1487.
  24. Andhare R.N., Raut M.K., Naik S.R. Evaluation of antiallergic and anti-anaphylactic activity of ethanolic extract of Sanseveiria trifasciata leaves (EEST) in rodents. J. Ethnopharmacol., 2012, vol. 142, no. 3, pp. 627–633. doi: 10.1016/j.jep.2012.05.007.
  25. Simmons-Boyce J.L., Tinto W.F., McLean S., Reynold W.F. Saponins from Furcraea selloa var. marginata. Fitoterapia, 2004, vol. 75, nos. 7–8, pp. 634–638.
  26. Cigerci I.H., Fidan A.F., Konuk M., Yuksel H., Kucukkurt I., Eryavuz A., Sozbilir N.B. The protective potential of Yucca schidigera (Sarsaponin 30) against nitrite-induced oxidative stress in rats. J. Nat. Med., 2009, vol. 63, no. 3, pp. 311–317. doi: 10.1007/s11418-009-0338-4.
  27. Ali M.S., Sharma G.C., Asplund R.O., Nevins M.P., Garb S. Isolation of antitumor polysaccharide fractions from Yucca glauca Nutt. (Lilliaceae). Growth, 1978, vol. 42, no. 2, pp. 213–223.
  28. Mimaki Y., Yokosuka A., Sashida Y., Steroidal glycosides from the aerial parts of Polianthes  tuberosa. J. Nat. Prod., 2000, vol. 63, no. 11, pp. 1519–1523.
  29. Itabashi M., Segawa K., Ikeda Y., Kondo S., Naganawa H., Koyano T., Umezawa K. A new bioactive steroidal saponin, furcreastatin, from the plant Furcraea foetida. Carbohydr. Res., 2000, vol. 323, nos. 1–4, pp. 57–62.
  30. Yokosuka A., Sano T., Hashimoto K., Sakagami H., Y. Mimaki. Steroidal glycosides from Furcraea foetida and their cytotoxic activity. Chem. Pharm. Bull. (Tokyo), 2009, vol. 57, no. 10, pp. 1161–1166.
  31. Karamova N.S., Zelenikhin P.V., Miroschnik N.B., Abdul-Hafeez E.Y., Zakirova Ya.N., Ilinskaya O.N. Apoptosis-inducing activity of Bacillus pumilus ribonuclease and some Egyptian medicinal plants extracts on human alveolar adenocarcinoma cells. Genes Cells, 2015, vol. 10, no. 3, pp. 62–67. (In Russian)

For citation: Kamalova Y.N., Shtyreva V.V., Essam Abdul-Hafeez, Omer H.M. Ibrahim, Zelenikhin P.V., Karamova N.S., Ilinskaya O.N. Cytotoxic and apoptosis-inducing activity of plants from the family Asparagaceae in relation to human alveolar adenocarcinoma cells. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2016, vol. 158, no. 3, pp. 338–350. (In Russian)


The content is available under the license Creative Commons Attribution 4.0 License.

Cytotoxic and Apoptosis-Inducing Activity of Plants from the Family Asparagaceae in Relation to Human Alveolar Adenocarcinoma Cells