2016, The possibility of assessment of skin irritation in rats with the use of simultaneous laser Doppler flowmetry (LDF) and oxygenation monitoring was investigated. The study was designed according to ISO 10993-10 using formaldehyde as a model irritant. 4-Hour application of 1 % formaldehyde induced significant local increase in skin microcirculation for the first 2 days by a factor of up to 2.4, whereas tissue oxygenation increased in much lower extent. The microcirculation was significantly inhibited by 10 % formaldehyde following treatment and then it gradually increased for 24 and 48 h of recovery period. The results correlated with visual evaluation of skin edema and erythema in treated zones. LDF/oxygenation signals provide early information on skin irritation and damage in rat model. The technique is valuable for preclinical/toxicological studies of chemical substances and medical materials.
2018, A new formulation of methylprednisolone succinate (MPS) with pre-oxidized carboxylated trifunctional block copolymer of ethylene oxide (EO) and propylene oxide (PO) (TBC?COOH) has been developed. TBC?COOH aggregates are spontaneously rearranged by MPS producing highly homogeneous mixed micelles (TBC?COOH/MPS) with hydrodynamic diameter of 19.6?0.3 nm, zeta potential of ?27.8?3.2 mV (pH 7.0) and with optimal weight ratio 1:6.3. The role of polypropylene oxide component in a copolymer and methylprednisolone succinylation in forming the mixed micelles was studied by comparing copolymers and corticosteroids of different structure. Micellar structure TBC?COOH/MPS is highly resistant to a dilution, temperature fluctuations and the presence of blood serum components. TBC?COOH promotes intrinsic cytotoxicity of increased concentrations of MPS for SH-SY5Y and PC-12 cells after 7-hour exposure attributed to enhanced cellular availability of the mixed micelles
2018, Synthesis and characterization of pyridoxine, nicotine and nicotinamide salts of dithiophosphoric acids as antibacterial agents against resistant wound infection