2024
2023
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Kurdy, W. Structure and functional potential of Arctic Sea sediment / W. Kurdy, G. Yakovleva, O. Ilinskaya // //Journal of General and Applied Microbiology. - 2023. - V.69, I.1. - P.24-33. https://doi 10.2323/jgam.2022.10.001
- Kurdy, W., Yakovleva, G., & Ilinskaya, O. (2023). Bacterial Biodiversity of the Kapova Karst Cave as a Source of Hydrolases Producers. European Journal of Biology, 82(2), 186-195. https://doi.org/10.26650/EurJBiol.2023.1349885
- Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave (Shulgan?Tash Nature Reserve, Bashkortostan), and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate (RNA, casein, starch).
2022
- 2,4,6-Trinitrotoluene (TNT) is the mostwidely used nitroaromatic compound and is highlyresistant to degradation. Most aerobic microorgan-isms reduce TNT to amino derivatives via forma-tion of nitroso- and hydroxylamine intermediates.Although pathways of TNT degradation are wellstudied, proteomic analysis of TNT-degrading bacte-ria was done only for some individual Gram-negative (PDF) Bacillus pumilus proteome changes in response to 2,4,6-trinitrotoluene-induced stress. Available from: trains. Here, we isolated a Gram-positive strainfrom TNT-contaminated soil, identified it as Bacil-lus pumilus using 16S rRNA sequencing, analyzed itsgrowth, the level of TNT transformation, ROS pro-duction, and revealed for the first time the bacillaryproteome changes at toxic concentration of TNT. Thetransformation of TNT at all studied concentrations(20?200 mg/L) followed the path of nitro groupsreduction with the formation of 4-amino-2,6-dinitro-toluene.
- William Kurdy, Galina Yakovleva, Olga Ilinskaya, Structure and Functional Potential of Arctic Sea Sediment Microbiota, The Journal of General and Applied Microbiology, 2023, Volume 69, Issue 1, Pages 24-33, Released on J-STAGE June 22, 2023, Advance online publication November 02, 2022, Online ISSN 1349-8037, Print ISSN 0022-1260, https://doi.org/10.2323/jgam.2022.10.001
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Yakovleva, G. Bacillus pumilus proteome changes in response to 2,4,6-trinitrotoluene-induced stress / G. Yakovleva, W. Kurdy, A. Gorbunova, I. Khilyas, G. Lochnit, O. Ilinskaya // Biodegradation. – 2022. – V.33. - № 6. – P.593-607 (10.1007/s10532-022-09997-8)
- Objects and structures made of organic glass require protection from damage caused by external factors. Light, humidity, temperature, dust pollution and, undoubtedly, microorganisms lead to the deterioration of optical and mechanical properties. Polysiloxane-based protective coatings, consisting of silicon?oxygen backbones linked together with organic side groups attached to the silicon atoms, are widely used. However, the polysiloxane coatings themselves also cannot avoid deterioration during operation that implies the constant development of new protective materials. Here, we created a new cross-linked polysiloxane that covers organic glasses to enhance their resistance to aggressive external factors, and investigated its own resistance to damage induced by micromycetes in natural tropical conditions and in the laboratory. It has been established that the surface of coatings in the tropics is prone to fouling with micromycetes, mainly of the genera Aspergillus and Penicillium, which
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