Effects of neuropeptide Y on electrical activity and myocardial contractility
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Effects of neuropeptide Y on electrical activity and myocardial contractility

A.A. Zverev a, T.A. Anikina a, N.G. Iskakov a, A.V. Krulova a, P.M. Masliukov b, J.P. Timmermans c, T.L. Zefirov a

a Kazan Federal University, Kazan, 420008 Russia

b Yaroslavl State Medical University, Yaroslavl, 150000 Russia

c University of Antwerp, Antwerp, 2000, Belgium

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Abstract

Neuropeptide Y (NPY) and agonist Leu(31)Pro(34)NPY were used to determine the type of NPY receptors involved in electrical activity and myocardial contraction. The experiments on the electrical activity of the right atrium showed that NPY and the selective agonist had negative inotropic and chronotropic actions through the activation of NPY1 receptor. The activation of NPY-receptors of right atrial cardiomyocytes with NPY leads to a change of the membrane potential and the amplitude-time parameters of the action potential. The experiments with the myocardial strips showed that NPY and the agonist produced the most potent effect at a concentration of 10–7 M. At this concentration, Leu(31)Pro(34)NPY showed the greatest positive inotropic effect on the contraction of the atria and ventricles.

Keywords: neuropeptide Y, action potential, contractility, rats

Acknowledgements. The study was supported by the Russian Foundation for Basic Research (project no. 18-34-00567) and performed according to the Russian Government Program of Competitive Growth of Kazan Federal University.

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Received

June 25, 2018

 

Zverev Aleksei Anatol'evich, Candidate of Biological Sciences, Associate Professor, Department of Human Health Protection

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: Alekcei5@rambler.ru

Anikina Tat'yana Andreevna, Doctor of Biological Sciences, Professor, Department of Human Health Protection

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: Taniya57vg1@@rambler.ru

Iskakov Nikita Georgievich, PhD Student, Department of Human Health Protection

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: nikitaiskakov1992@mail.ru

Krulova Alevtina Vasil'evna, Candidate of Biological Sciences, Associate Professor, Department of Human Health Protection

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: krylova.alevtina@gmail.com

Masliukov Petr Mikhailovich, Doctor of Medical Sciences, Professor, Department of Normal Physiology with Biophysics

Yaroslavl State Medical University

ul. Revolyutsionnaya, 5, Yaroslavl, 150000 Russia

E-mail: mpm@ysmu.ru

Timmermans Jean Pierre, PhD, Professor, Department of Veterinary Sciences

University of Antwerp

St-Jacobsmarkt 9-13, Antwerp, 2000, Belgium

Zefirov Timur L'vovich, Doctor of Medical Sciences, Professor, Department of Human Health Protection

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: zefirovtl@mail.ru

 

For citation: Zverev A.A., Anikina T.A., Iskakov N.G., Krulova A.V., Masliukov P.M., Timmermans J.P., Zefirov T.L. Effects of neuropeptide Y on electrical activity and myocardial contractility. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2018, vol. 160, no. 4, pp. 601–612.

Для цитирования: Zverev A.A., Anikina T.A., Iskakov N.G., Krulova A.V., Masliukov P.M., Timmermans J.P., Zefirov T.L. Effects of neuropeptide Y on electrical activity and myocardial contractility // Учен. зап. Казан. ун-та. Сер. Естеств. науки. – 2018. – Т. 160, кн. 4. – С. 601–612.

 

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