Effects of local hypothermia on spinal cord injury in rats
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Effects of local hypothermia on spinal cord injury in rats

T.V. Baltina a, D.I. Silantyeva a, E.Yu. Loban a, M.V. Raimova a, I.A. Lavrov a,b

a Kazan Federal University, Kazan, 420008 Russia

b Mayo Clinic, Rochester, MN, 55905 USA

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Abstract

The aim of this study was to evaluate possible neuroprotective effects of local hypothermia on the functional state of the spinal motor centers of the calf muscles after a contusion injury of the spinal cord (SCI) in rats. It was assumed that local hypothermia can decrease the secondary damage of SCI and can be considered as a way to treat SCI. The experiments were carried out on 49 adult Wistar rats with the weight of 250–300 g. All procedures were performed in accor­dance with the bioethics rules. SCI was performed in the region of Th8–Th9 vertebrae. The analysis of the motor functions assessed in the open field test and of the parameters of the motor evoked potentials of hindlimb muscles induced by epidural stimulation of the spinal cord was carried out during a month period after the injury. It was shown that the locomotor ability of rats in the open field test did not differ between the group with SCI and the group with local hypothermia. The amplitude of motor evoked potentials of the calf muscles significantly decreased and the thresholds of inducing these potentials increased in the group with hypothermia. Thus, the results suggest that local hypothermia after SCI can delay the development of functional excitation of the neuro-motor pathways induced by SCI. The obtained data can be useful for developing new therapeutic approaches, which are necessary to delay the pathophysiological processes associated with the secondary damage, as well as to limit the expression of neurological dysfunction induced by SCI.

Keywords: spinal cord injury, motor evoked potentials, epidural stimulation, locomotor activity, local hypothermia

Acknowledgments. The study was supported by the Russian Foundation for Basic Research (project no. 17-04-01746).

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Recieved

June 22, 2018

Baltina Tatiana Velerievna, Candidate of Biological Sciences, Associate Professor of Department of Human and Animal Physiology

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: tvbaltina@gmail.com

Silantyeva Dinara Irekovna, Candidate of Biological Sciences, Senior Research Fellow of Rehabilitation in Movement Disorders OpenLab

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: tvbaltina@gmail.com

Loban Ekaterina Yurievna, PhD Student of Department of Human and Animal Physiology

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: kety-smile@mail.ru

Raimova Maria Vasilievna, Student of Department of Human and Animal Physiology

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

E-mail: raimovamasha@yandex.ru

Lavrov Igor Aleksandrovich, Candidate of Medical Sciences, Senior Research Fellow of Rehabilitation in Movement Disorders OpenLab, Head of Department of Physiology and Biomedical Engineering

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

Mayo Clinic

200 First St. SW Rochester, MN 55905 United States

E-mail: igor.lavrov@gmail.com

 

For citation: Baltina T.V., Silantyeva D.I., Loban E.Yu., Raimova M.V., Lavrov I.A. Effects of local hypothermia on spinal cord injury in rats. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2018, vol. 160, no. 4, pp. 630–644.

Для цитирования: Baltina T.V., Silantyeva D.I., Loban E.Yu., Raimova M.V., Lavrov I.A. Effects of local hypothermia on spinal cord injury in rats // Учен. зап. Казан. ун-та. Сер. Естеств. науки. – 2018. – Т. 160, кн. 4. – С. 630–644.

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