Inhibition of inward rectifier potassium currents by chloroquine causes significant electrophysiological changes in the rat thoracic veins myocardium
A.D. Ivanova a, V.S. Kuzmin a,b
a Moscow State University, Moscow, 119991 Russia
b Pirogov Russian National Research Medical University, Moscow, 117997 Russia
Abstract
Thoracic (caval and pulmonary) vein myocardial tissue is considered as a source of ectopic activity capable of initiating atrial fibrillation. The reasons which underline thoracic vein proarrhythmicity may be related to the expression level of the inward rectifier potassium current (IK1) critical for stable resting membrane potential (RMP) maintenance. In the present study the effect of IK1 inhibition by chloroquine (5 ?M) on the membrane resting and action potentials (AP) were recorded in the superior caval, pulmonary veins and left atria tissue preparations. There were no differences in RMP level: the AP duration in veins was significantly longer than in the atrium under control conditions. The chloroquine application increased the AP duration greater in the vein myocardium as compared to the left atrium. The study results allow to suggest that the thoracic vein myocardium has a lower density of IK1 current relatively to the working atrial myocardium.
Keywords: action potential, thoracic vein myocardium, extracardiac myocardium, atrial fibrillation, inward rectifier potassium current, spontaneous automaticity
Acknowledgments. This study is supported by the Russian Foundation for Basic Research (project no. 17-04-01921-a).
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Received
July 1, 2018
Ivanova Alexandra Dmitrievna, PhD Student, Department of Human and Animal Physiology
Moscow State University
Leninskie Gory, 1, Moscow, 119991 Russia
E-mail: ivanova.aleksandra.2012@post.bio.msu.ru
Kuzmin Vlad Stephanovich, Candidate of Biological Sciences, Assistant Professor, Department of Human and Animal Physiology; Researcher, Department of Physiology
Moscow State University
Leninskie Gory, 1, Moscow, 119991 Russia
Pirogov Russian National Research Medical University
ul. Ostrovitjanova, 1, Moscow, 117997 Russia
E-mail: ku290381@gmail.com
For citation: Ivanova A.D., Kuzmin V.S. Inhibition of inward rectifier potassium currents by chloroquine causes significant electrophysiological changes in the rat thoracic veins myocardium. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2018, vol. 160, no. 4, pp. 645–653.
Для цитирования: Ivanova A.D., Kuzmin V.S. Inhibition of inward rectifier potassium currents by chloroquine causes significant electrophysiological changes in the rat thoracic veins myocardium // Учен. зап. Казан. ун-та. Сер. Естеств. науки. – 2018. – Т. 160, кн. 4. – С. 645–653.
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