KFU. Card publication. Zeolites as Carriers of Antitumor Ribonuclease Binase

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

ZEOLITES AS CARRIERS OF ANTITUMOR RIBONUCLEASE BINASE

Form of presentation

Articles in international journals and collections

Year of publication

2019

Язык

английский

Authors, employees of KFU

Zelenikhin Pavel Valerevich, author

Ilinskaya Olga Nikolaevna, author

Lopatin Oleg Nikolaevich, author

Khodzhaeva Vera Sergeevna, author

Bibliographic description in the original language

Khojaewa V. Zeolites as Carriers of Antitumor Ribonuclease Binase / Khojaewa V, Lopatin O, Zelenikhin P, Ilinskaya O // Front Pharmacol. – 2019. – V.10, P. 442. doi:10.3389/fphar.2019.00442. eCollection 2019.

Annotation

Natural and synthetic zeolites have many applications in biomedicine and nutrition. Due to its properties, zeolites can absorb therapeutically active proteins and release them under physiological conditions. In this study we tested the clinoptilolite, chabazite, and natrolite ability to be loaded by antitumor ribonuclease binase and the cytotoxicity of the obtained complexes. We found the optimal conditions for binase loading into zeolites and established the dynamic of its release. Cytotoxic effects of zeolite-binase complexes toward colorectal cancer Caco2 cells were characterized after 24 and 48 h of incubation with cells using MTT-test. Zeolites were toxic by itselfs and reduced cells viability by 30% (clinoptilolite), 40% (chabazite), and 70% (natrolite) after 48 h of incubation. Binase complexes with clinoptilolite as well as chabazite always demonstrated enhanced toxicity (up to 57 and 60% for clinoptilolite and chabazite, respectively) in comparison with binase and zeolites separately. Our results contribute to the perspective development of binase-based complexes for therapy of colorectal cancer for or the treatment of malignant skin neoplasms where the complexes can be used in pasty form.

Keywords

binase, chabazite, clinoptilolite, cytotoxic RNAse, immobilization, natrolite, zeolites

The name of the journal

FRONTIERS IN PHARMACOLOGY

URL

https://www.frontiersin.org/articles/10.3389/fphar.2019.00442/full

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=205905&p_lang=2

Resource files

File name	Size (MB)	Format
445483_Frontiers_in_Pharmacology.PDF	2,29	PDF	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Zelenikhin Pavel Valerevich	ru_RU
dc.contributor.author	Ilinskaya Olga Nikolaevna	ru_RU
dc.contributor.author	Lopatin Oleg Nikolaevich	ru_RU
dc.contributor.author	Khodzhaeva Vera Sergeevna	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Khojaewa V. Zeolites as Carriers of Antitumor Ribonuclease Binase / Khojaewa V, Lopatin O, Zelenikhin P, Ilinskaya O // Front Pharmacol. – 2019. – V.10, P. 442. doi:10.3389/fphar.2019.00442. eCollection 2019.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=205905&p_lang=2	ru_RU
dc.description.abstract	FRONTIERS IN PHARMACOLOGY	ru_RU
dc.description.abstract	Natural and synthetic zeolites have many applications in biomedicine and nutrition. Due to its properties, zeolites can absorb therapeutically active proteins and release them under physiological conditions. In this study we tested the clinoptilolite, chabazite, and natrolite ability to be loaded by antitumor ribonuclease binase and the cytotoxicity of the obtained complexes. We found the optimal conditions for binase loading into zeolites and established the dynamic of its release. Cytotoxic effects of zeolite-binase complexes toward colorectal cancer Caco2 cells were characterized after 24 and 48 h of incubation with cells using MTT-test. Zeolites were toxic by itselfs and reduced cells viability by 30% (clinoptilolite), 40% (chabazite), and 70% (natrolite) after 48 h of incubation. Binase complexes with clinoptilolite as well as chabazite always demonstrated enhanced toxicity (up to 57 and 60% for clinoptilolite and chabazite, respectively) in comparison with binase and zeolites separately. Our results contribute to the perspective development of binase-based complexes for therapy of colorectal cancer for or the treatment of malignant skin neoplasms where the complexes can be used in pasty form.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	binase	ru_RU
dc.subject	chabazite	ru_RU
dc.subject	clinoptilolite	ru_RU
dc.subject	cytotoxic RNAse	ru_RU
dc.subject	immobilization	ru_RU
dc.subject	natrolite	ru_RU
dc.subject	zeolites	ru_RU
dc.title	Zeolites as Carriers of Antitumor Ribonuclease Binase	ru_RU
dc.type	Articles in international journals and collections	ru_RU