KFU. Card publication. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies

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GENETIC TOOLS FOR TAGGING GRAM-NEGATIVE BACTERIA WITH MCHERRY FOR VISUALIZATION IN VITRO AND IN NATURAL HABITATS, BIOFILM AND PATHOGENICITY STUDIES

Form of presentation

Articles in international journals and collections

Year of publication

2011

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Authors, employees of KFU

Validov Shamil Zavdatovich, author

Bibliographic description in the original language

Lagendijk EL, Validov S, Lamers GEM, de Weert S, Bloemberg GV. 2010. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. Fems Microbiology Letters 305:81-90.

Annotation

Live-cell imaging techniques are essential to gain a better understanding of microbial functioning in natural systems, for example in biofilms. Autofluorescent proteins, such as the green fluorescent protein (GFP) and the red fluorescent protein (DsRed), are valuable tools for studying microbial communities in their natural environment. Because of the functional limitations of DsRed such as slow maturation and low photostability, new and improved variants were created such as mCherry. In this study, we developed genetic tools for labeling Gram-negative bacteria in order to visualize them in vitro and in their natural environment without the necessity of antibiotic pressure for maintenance. mcherry was cloned into two broad host-range cloning vectors and a pBK-miniTn7 transposon under the constitutive expression of the tac promoter. The applicability of the different constructs was shown in Escherichia coli, various Pseudomonas spp. and Edwardsiella tarda. The expression of mcherry was

Keywords

Pseudomonas, Live-cell imaging

The name of the journal

FEMS Microbiology Letters

URL

http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2010.01916.x/pdf

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=94463&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Validov Shamil Zavdatovich	ru_RU
dc.date.accessioned	2011-01-01T00:00:00Z	ru_RU
dc.date.available	2011-01-01T00:00:00Z	ru_RU
dc.date.issued	2011	ru_RU
dc.identifier.citation	Lagendijk EL, Validov S, Lamers GEM, de Weert S, Bloemberg GV. 2010. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. Fems Microbiology Letters 305:81-90.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=94463&p_lang=2	ru_RU
dc.description.abstract	FEMS Microbiology Letters	ru_RU
dc.description.abstract	Live-cell imaging techniques are essential to gain a better understanding of microbial functioning in natural systems, for example in biofilms. Autofluorescent proteins, such as the green fluorescent protein (GFP) and the red fluorescent protein (DsRed), are valuable tools for studying microbial communities in their natural environment. Because of the functional limitations of DsRed such as slow maturation and low photostability, new and improved variants were created such as mCherry. In this study, we developed genetic tools for labeling Gram-negative bacteria in order to visualize them in vitro and in their natural environment without the necessity of antibiotic pressure for maintenance. mcherry was cloned into two broad host-range cloning vectors and a pBK-miniTn7 transposon under the constitutive expression of the tac promoter. The applicability of the different constructs was shown in Escherichia coli, various Pseudomonas spp. and Edwardsiella tarda. The expression of mcherry was	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Pseudomonas	ru_RU
dc.subject	Live-cell imaging	ru_RU
dc.title	Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies	ru_RU
dc.type	Articles in international journals and collections	ru_RU