KFU. Card publication. Usage of Plasmid Vector Carrying vegf and fgf2 Genes after Spinal Cord Injury in Rats

KAZAN
FEDERAL UNIVERSITY

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USAGE OF PLASMID VECTOR CARRYING VEGF AND FGF2 GENES AFTER SPINAL CORD INJURY IN RATS

Form of presentation

Articles in international journals and collections

Year of publication

2013

Authors, employees of KFU

Mukhamedshina Yana Olegovna, author

Rizvanov Albert Anatolevich, author

Salafutdinov Ilnur Ildusovich, author

Other authors

G.F. Shaymardanova; Y.A. Chelyshev

Bibliographic description in the original language

Shaymardanova G.F. Usage of Plasmid Vector Carrying vegf and fgf2 Genes after Spinal Cord Injury in Rats / G.F. Shaymardanova, Y.O. Mukhamedshina, I.I. Salafutdinov, A.A. Rizvanov, Y.A. Chelyshev // Bulletin of Experimental Biology and Medicine. – Vol.154, No.4. – P.544-547.

Annotation

Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of the same plasmid into the lesion area. The results suggest that the delivery of therapeutic genes vegf and fgf2 in cells is more effective than direct injection of plasmid DNA with the same genes (judging from the number of myelinated fibers). Better tissue preservation and motor function recovery in experiments with direct injection of plasmid pBud-VEGF-FGF2 suggest that direct gene therapy seems to be an effective additional procedure to the method of gene delivery with transfected stem and progenitor cells.

Keywords

spinal cord injury, umbilical cord blood cells, transfection, plasmid

The name of the journal

Bulletin of Experimental Biology and Medicine

URL

http://download.springer.com/static/pdf/421/art%253A10.1007%252Fs10517-013-1996-5.pdf?auth66=1418901404_4ec4f3388cbbe0a21192aabd4e09902f&ext=.pdf

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=94153&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Mukhamedshina Yana Olegovna	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Salafutdinov Ilnur Ildusovich	ru_RU
dc.date.accessioned	2013-01-01T00:00:00Z	ru_RU
dc.date.available	2013-01-01T00:00:00Z	ru_RU
dc.date.issued	2013	ru_RU
dc.identifier.citation	Shaymardanova G.F. Usage of Plasmid Vector Carrying vegf and fgf2 Genes after Spinal Cord Injury in Rats / G.F. Shaymardanova, Y.O. Mukhamedshina, I.I. Salafutdinov, A.A. Rizvanov, Y.A. Chelyshev // Bulletin of Experimental Biology and Medicine. – Vol.154, No.4. – P.544-547.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=94153&p_lang=2	ru_RU
dc.description.abstract	Bulletin of Experimental Biology and Medicine	ru_RU
dc.description.abstract	Using rat model of spinal cord contusion injury at TVIII, we compared the effectiveness of immediate single transplantation of human mononuclear umbilical cord blood cells transfected with pBud-VEGF-FGF2 plasmid and immediate direct injection of the same plasmid into the lesion area. The results suggest that the delivery of therapeutic genes vegf and fgf2 in cells is more effective than direct injection of plasmid DNA with the same genes (judging from the number of myelinated fibers). Better tissue preservation and motor function recovery in experiments with direct injection of plasmid pBud-VEGF-FGF2 suggest that direct gene therapy seems to be an effective additional procedure to the method of gene delivery with transfected stem and progenitor cells.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	spinal cord injury	ru_RU
dc.subject	umbilical cord blood cells	ru_RU
dc.subject	transfection	ru_RU
dc.subject	plasmid	ru_RU
dc.title	Usage of Plasmid Vector Carrying vegf and fgf2 Genes after Spinal Cord Injury in Rats	ru_RU
dc.type	Articles in international journals and collections	ru_RU