KFU. Card publication. Genetic Modification of Adipose Derived Stem Cells with Recombinant Plasmid DNA pBud-VEGF-FGF2 Results in Increased of IL-8 and MCP-1 Secretion

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GENETIC MODIFICATION OF ADIPOSE DERIVED STEM CELLS WITH RECOMBINANT PLASMID DNA PBUD-VEGF-FGF2 RESULTS IN INCREASED OF IL-8 AND MCP-1 SECRETION

Form of presentation

Articles in international journals and collections

Year of publication

2014

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Authors, employees of KFU

Masgutov Ruslan Faridovich, author

Salafutdinov Ilnur Ildusovich, author

Soloveva Valeriya Vladimirovna, author

Tazetdinova Leysan Gazinurovna, author

Khaybullina Svetlana Francevna, author

Authors, postgraduates KFU

Tazetdinova Leysan Gazinurovna, postgraduate kfu

Bibliographic description in the original language

Solovyeva V.V. Genetic Modification of Adipose Derived Stem Cells with Recombinant Plasmid DNA pBud-VEGF-FGF2 Results in Increased of IL-8 and MCP-1 Secretion / V.V. Solovyeva, I.I. Salafutdinov, L.G. Tazetdinova, R.F. Masgutov, S.F. Khaiboullina, A.A. Rizvanov // Journal of pure and applied microbiology. – 2014. – Vol.8, Spl.Edn.2. – P.523-528.

Annotation

Bacterial plasmid DNA is often used in gene therapy application as a vector for delivering recombinant genes into eukaryotic cells. In this work we study effect of plasmid DNA encoding pro-angiogenic growth factors VEGF and FGF2 on cytokine production of human adipose derived stem cells (ADSC). Adipose tissue contain progenitor and stem cells with angiogenic potential which are considered promising cell source for treating ischemic conditions. Genetic modification of human ADSC with recombinant plasmids encoding therapeutic growth factors might change therapeutic potential of these cells. We report that genetic modification of ADSC with recombinant plasmid pBud-VEGF-FGF2 results in expected increased of VEGF secretion and also results in increase of IL-8 and MCP-1 secretion into culture medium.

Keywords

bacterial plasmid DNA, cytokines, chemokines, human adipose derived stem cells, stromal vascular fraction, vascular endothelial growth factor, basic fibroblast growth factor, genetic modification

The name of the journal

Journal of pure and applied microbiology

URL

http://microbiologyjournal.org/jmabsread.php?snoid=2279&month=&year=

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=93677&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Masgutov Ruslan Faridovich	ru_RU
dc.contributor.author	Salafutdinov Ilnur Ildusovich	ru_RU
dc.contributor.author	Soloveva Valeriya Vladimirovna	ru_RU
dc.contributor.author	Tazetdinova Leysan Gazinurovna	ru_RU
dc.contributor.author	Khaybullina Svetlana Francevna	ru_RU
dc.contributor.author	Tazetdinova Leysan Gazinurovna	ru_RU
dc.date.accessioned	2014-01-01T00:00:00Z	ru_RU
dc.date.available	2014-01-01T00:00:00Z	ru_RU
dc.date.issued	2014	ru_RU
dc.identifier.citation	Solovyeva V.V. Genetic Modification of Adipose Derived Stem Cells with Recombinant Plasmid DNA pBud-VEGF-FGF2 Results in Increased of IL-8 and MCP-1 Secretion / V.V. Solovyeva, I.I. Salafutdinov, L.G. Tazetdinova, R.F. Masgutov, S.F. Khaiboullina, A.A. Rizvanov // Journal of pure and applied microbiology. – 2014. – Vol.8, Spl.Edn.2. – P.523-528.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=93677&p_lang=2	ru_RU
dc.description.abstract	Journal of pure and applied microbiology	ru_RU
dc.description.abstract	Bacterial plasmid DNA is often used in gene therapy application as a vector for delivering recombinant genes into eukaryotic cells. In this work we study effect of plasmid DNA encoding pro-angiogenic growth factors VEGF and FGF2 on cytokine production of human adipose derived stem cells (ADSC). Adipose tissue contain progenitor and stem cells with angiogenic potential which are considered promising cell source for treating ischemic conditions. Genetic modification of human ADSC with recombinant plasmids encoding therapeutic growth factors might change therapeutic potential of these cells. We report that genetic modification of ADSC with recombinant plasmid pBud-VEGF-FGF2 results in expected increased of VEGF secretion and also results in increase of IL-8 and MCP-1 secretion into culture medium.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	bacterial plasmid DNA	ru_RU
dc.subject	cytokines	ru_RU
dc.subject	chemokines	ru_RU
dc.subject	human adipose derived stem cells	ru_RU
dc.subject	stromal vascular fraction	ru_RU
dc.subject	vascular endothelial growth factor	ru_RU
dc.subject	basic fibroblast growth factor	ru_RU
dc.subject	genetic modification	ru_RU
dc.title	Genetic Modification of Adipose Derived Stem Cells with Recombinant Plasmid DNA pBud-VEGF-FGF2 Results in Increased of IL-8 and MCP-1 Secretion	ru_RU
dc.type	Articles in international journals and collections	ru_RU