KFU. Card publication. The particularities of protein fraction in the apoptosis of lymphocytes of patients with asthma

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FEDERAL UNIVERSITY

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THE PARTICULARITIES OF PROTEIN FRACTION IN THE APOPTOSIS OF LYMPHOCYTES OF PATIENTS WITH ASTHMA

Form of presentation

Articles in international journals and collections

Year of publication

2013

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Authors, employees of KFU

Abramova Zinaida Ivanovna, author

Bibliographic description in the original language

Vodounon A.C. The particularities of protein fraction in the apoptosis of lymphocytes of patients with asthma/A.C. Vodounon, Z.I. Abramova, N. Aikou, A. Sezan, S.O. Kotchoni, S.Akpona, L. Baba-Moussa. //Pakistan Journal of Biological Sciences.-2013 .-V.16,�24.-P.1873-1883.

Annotation

Abstract: The emergence of various diseases specifically severe diseases such as bronchial asthma is associated with apoptosis of lymphocytes. One of the major biochemical features of apoptosis is chromosome DNA fragmentation implemented by apoptotic nucleases. The inactivation of these apoptotic nucleases produces undigested DNA and is linked to a number of autoimmune disorders. Instead of this we have studied the enzymatic activities of the cytoplasmic and nucleic proteins of lymphocytes from healthy donors and patients with bronchial asthma. The study of enzymatic activities of the nuclease of lymphocytes was assessed by flow cytometry, spectrofluorimetry and electrophoresis method in agarose gel. In the peripheral blood cells of healthy donors undergoing apoptosis, we found a DNAse activated by Ca2+ and Mg2+ ions. Lymphocytes of patients with bronchial asthma contain DNAses, the activity of which depends on the seriousness of the desease. In patients cells, the activity of the Mn2+-dependent DNAse increases, whereas the activity of the Ca2+, Mg2+-dependent DNase decreases. Taking into consideration the role of the Ca2+, Mg2+-dependent DNAse in apoptosis, we can propose that there is a link between the reduction of the rate of apoptosis of lymphocytes in patients with bronchial asthma and the dysfunction of the induction of “apoptotical” Ca2+, Mg2+-dependent nuclease. According to the results obtained, we can assume that why apoptosis of lymphocytes resist in patients with bronchial asthma is a reduction in concentration of endocellular calcium and an increase of manganese ions content, which results in the triggering of activation mechanism for Mn2+-dependent endonuclease activity. This leads to the change of DNA fragmentation nature in lymphocytes and as a consequence, to disorders in process of apoptotic bodies' formation, thus, hindering apoptosis of lymphocytes in patients with bronchial asthma

Keywords

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The name of the journal

Pakistan Journal of Biological Sciences.

URL

http://www.scopus.com/record/display.uri?eid=2-s2.0-84883851894&origin=resultslist&sort=plf-f&src=s&st1=Abramova&st2=Z.I.&nlo=1&nlr=20&nls=count-f&sid=4354CABE9334ED325634DEC52C78FCF1.aXczxbyuHHiXgaIW6Ho7g%3a973&sot=anl&sdt=aut&sl=47&s=AU-ID%28%22Abramova%

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=63624&p_lang=2

Resource files

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Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.date.accessioned	2013-01-01T00:00:00Z	ru_RU
dc.date.available	2013-01-01T00:00:00Z	ru_RU
dc.date.issued	2013	ru_RU
dc.identifier.citation	Vodounon A.C. The particularities of protein fraction in the apoptosis of lymphocytes of patients with asthma/A.C. Vodounon, Z.I. Abramova, N. Aikou, A. Sezan, S.O. Kotchoni, S.Akpona, L. Baba-Moussa. //Pakistan Journal of Biological Sciences.-2013 .-V.16,�24.-P.1873-1883.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=63624&p_lang=2	ru_RU
dc.description.abstract	Pakistan Journal of Biological Sciences.	ru_RU
dc.description.abstract	Abstract: The emergence of various diseases specifically severe diseases such as bronchial asthma is associated with apoptosis of lymphocytes. One of the major biochemical features of apoptosis is chromosome DNA fragmentation implemented by apoptotic nucleases. The inactivation of these apoptotic nucleases produces undigested DNA and is linked to a number of autoimmune disorders. Instead of this we have studied the enzymatic activities of the cytoplasmic and nucleic proteins of lymphocytes from healthy donors and patients with bronchial asthma. The study of enzymatic activities of the nuclease of lymphocytes was assessed by flow cytometry, spectrofluorimetry and electrophoresis method in agarose gel. In the peripheral blood cells of healthy donors undergoing apoptosis, we found a DNAse activated by Ca2+ and Mg2+ ions. Lymphocytes of patients with bronchial asthma contain DNAses, the activity of which depends on the seriousness of the desease. In patients cells, the activity of the Mn2+-dependent DNAse increases, whereas the activity of the Ca2+, Mg2+-dependent DNase decreases. Taking into consideration the role of the Ca2+, Mg2+-dependent DNAse in apoptosis, we can propose that there is a link between the reduction of the rate of apoptosis of lymphocytes in patients with bronchial asthma and the dysfunction of the induction of “apoptotical” Ca2+, Mg2+-dependent nuclease. According to the results obtained, we can assume that why apoptosis of lymphocytes resist in patients with bronchial asthma is a reduction in concentration of endocellular calcium and an increase of manganese ions content, which results in the triggering of activation mechanism for Mn2+-dependent endonuclease activity. This leads to the change of DNA fragmentation nature in lymphocytes and as a consequence, to disorders in process of apoptotic bodies' formation, thus, hindering apoptosis of lymphocytes in patients with bronchial asthma	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	��	ru_RU
dc.subject	� ��	ru_RU
dc.subject	��	ru_RU
dc.subject	��	ru_RU
dc.title	The particularities of protein fraction in the apoptosis of lymphocytes of patients with asthma	ru_RU
dc.type	Articles in international journals and collections	ru_RU