KFU. Card publication. Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test

- RUS
- 中文
- ES

USE OF A MULTIPLEX ENZYME-LINKED IMMUNOSORBENT ASSAY TO DETECT A SUBPOPULATION OF MYCOBACTERIUM BOVIS-INFECTED ANIMALS DEEMED NEGATIVE OR INCONCLUSIVE BY THE SINGLE INTRADERMAL COMPARATIVE TUBERCULIN SKIN TEST

Form of presentation

Articles in international journals and collections

Year of publication

2011

��

��

Authors, employees of KFU

Shuralev Eduard Arkadevich, author

Other authors

Clarke John , author

Davis William C. , author

Duignan Anthony , author

Good Margaret , author

Kenny Kevin , author

Kwok Hang Fai , author

Whelan Clare , author

Bibliographic description in the original language

Whelan C., Shuralev E., Kwok H.F., Kenny K., Duignan A., Good M., Davis W.C., Clarke J. Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test // J Vet Diagn Invest. 2011. 23(3): 499-503. DOI: 10.1177/1040638711403410

Annotation

The present study was undertaken to determine if a multiplex assay can improve the level of detection of infected animals when used in combination with the SICTT. The Enferplex TB assay is a multi-antigen ELISA designed for the detection of antibody in animals at different stages of infection and disease. Sixty cattle that were confirmed by histopathology and/or culture to be infected with M. bovis and that were SICTT negative (43.3%) or difficult to evaluate (56.7% inconclusive) were used in the study. Fifty-three (88.3%) of the animals were positive in multiplex ELISA. The results show that the level of detection of M. bovis–infected animals can be improved by the combined use of the SICTT and the multiplex ELISA.

Keywords

bovine tuberculosis, chemiluminescent, multiplex, Mycobacterium bovis, single intradermal comparative tuberculin test

The name of the journal

J VET DIAGN INVEST

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/149794/Whelan_2011.pdf?sequence=1&isAllowed=y

URL

https://journals.sagepub.com/doi/10.1177/1040638711403410

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=35750&p_lang=2

Resource files

File name	Size (MB)	Format
Whelan_2011.pdf	0,09	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Shuralev Eduard Arkadevich	ru_RU
dc.contributor.author	Clarke John	ru_RU
dc.contributor.author	Davis William C.	ru_RU
dc.contributor.author	Duignan Anthony	ru_RU
dc.contributor.author	Good Margaret	ru_RU
dc.contributor.author	Kenny Kevin	ru_RU
dc.contributor.author	Kwok Hang Fai	ru_RU
dc.contributor.author	Whelan Clare	ru_RU
dc.date.accessioned	2011-01-01T00:00:00Z	ru_RU
dc.date.available	2011-01-01T00:00:00Z	ru_RU
dc.date.issued	2011	ru_RU
dc.identifier.citation	Whelan C., Shuralev E., Kwok H.F., Kenny K., Duignan A., Good M., Davis W.C., Clarke J. Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test // J Vet Diagn Invest. 2011. 23(3): 499-503. DOI: 10.1177/1040638711403410	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=35750&p_lang=2	ru_RU
dc.description.abstract	J VET DIAGN INVEST	ru_RU
dc.description.abstract	The present study was undertaken to determine if a multiplex assay can improve the level of detection of infected animals when used in combination with the SICTT. The Enferplex TB assay is a multi-antigen ELISA designed for the detection of antibody in animals at different stages of infection and disease. Sixty cattle that were confirmed by histopathology and/or culture to be infected with M. bovis and that were SICTT negative (43.3%) or difficult to evaluate (56.7% inconclusive) were used in the study. Fifty-three (88.3%) of the animals were positive in multiplex ELISA. The results show that the level of detection of M. bovis–infected animals can be improved by the combined use of the SICTT and the multiplex ELISA.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	bovine tuberculosis	ru_RU
dc.subject	chemiluminescent	ru_RU
dc.subject	multiplex	ru_RU
dc.subject	Mycobacterium bovis	ru_RU
dc.subject	single intradermal comparative tuberculin test	ru_RU
dc.title	Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test	ru_RU
dc.type	Articles in international journals and collections	ru_RU