KFU. Card publication. Second harmonic generation for brain imaging: pathology-related studies

KAZAN
FEDERAL UNIVERSITY

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SECOND HARMONIC GENERATION FOR BRAIN IMAGING: PATHOLOGY-RELATED STUDIES

Form of presentation

Articles in international journals and collections

Year of publication

2025

Язык

английский

Authors, employees of KFU

Kiyasov Andrey Pavlovich, author

Melnikova Anastasiya Aleksandrovna, author

Pavelev Mikhail Nikolaevich, author

Titova Angelina Andreevna, author

Bibliographic description in the original language

Paveliev M. Second harmonic generation for brain imaging: pathology-related studies / M. Paveliev, A. Melnikova1, D.V. Samigullin, A.A. Egorchev, A.A. Titova, A.P. Kiyasov, I.Yu. Popova, V.Parpura, A.V. Aganov // Biophysical Reviews. – 2025. – V. ?. – 20 p. doi.org/10.1007/s12551-025-01370-2

Annotation

Microscopy of the brain has been facing problems of contrast and thick tissue imaging. Second harmonic generation (SHG) is a non-linear effect of the light interaction with the imaged material, resulting in photon emission at half the wavelength of the absorbed light. SHG microscopy provides an unprecedented opportunity for imaging collagen and other noncentrosymmetric protein fibrils in unstained thick tissue samples and in the live brain via a regular multiphoton setup. This opens a remarkable methodological window for imaging pathological processes of high importance, including brain trauma, fibrosis, tumorigenesis, and neuroimplant-induced foreign body response. Moreover, SHG is a valuable tool for imaging astrocytes and nerve fiber microtubules. Third harmonic generation enhanced by three-photon resonance with the Soret band of hemoglobin is combined with SHG to resolve the microstructure of blood vessel walls and astrocyte-process endfeet on gliovascular interfaces. Here, we review current state-of-the-art methods in the field of brain imaging applications of SHG, including research on brain and spinal cord injury, glioma, ischemia, Alzheimer's disease, neuroimplantation, and brain meninges. We then address the method development perspective in the broader context of other tissue pathologies. Finally, we account for recent progress in artificial intelligence applications for SHG microscopy data analysis.

Keywords

Brain imaging ? Second harmonic generation ? Collagen ? Glioma ? Brain‒computer interface ? Machine learning

The name of the journal

Biophysical Reviews

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=318020&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Melnikova Anastasiya Aleksandrovna	ru_RU
dc.contributor.author	Pavelev Mikhail Nikolaevich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.date.accessioned	2025-01-01T00:00:00Z	ru_RU
dc.date.available	2025-01-01T00:00:00Z	ru_RU
dc.date.issued	2025	ru_RU
dc.identifier.citation	Paveliev M. Second harmonic generation for brain imaging: pathology-related studies / M. Paveliev, A. Melnikova1, D.V. Samigullin, A.A. Egorchev, A.A. Titova, A.P. Kiyasov, I.Yu. Popova, V.Parpura, A.V. Aganov // Biophysical Reviews. – 2025. – V. ?. – 20 p. doi.org/10.1007/s12551-025-01370-2	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=318020&p_lang=2	ru_RU
dc.description.abstract	Biophysical Reviews	ru_RU
dc.description.abstract	Microscopy of the brain has been facing problems of contrast and thick tissue imaging. Second harmonic generation (SHG) is a non-linear effect of the light interaction with the imaged material, resulting in photon emission at half the wavelength of the absorbed light. SHG microscopy provides an unprecedented opportunity for imaging collagen and other noncentrosymmetric protein fibrils in unstained thick tissue samples and in the live brain via a regular multiphoton setup. This opens a remarkable methodological window for imaging pathological processes of high importance, including brain trauma, fibrosis, tumorigenesis, and neuroimplant-induced foreign body response. Moreover, SHG is a valuable tool for imaging astrocytes and nerve fiber microtubules. Third harmonic generation enhanced by three-photon resonance with the Soret band of hemoglobin is combined with SHG to resolve the microstructure of blood vessel walls and astrocyte-process endfeet on gliovascular interfaces. Here, we review current state-of-the-art methods in the field of brain imaging applications of SHG, including research on brain and spinal cord injury, glioma, ischemia, Alzheimer's disease, neuroimplantation, and brain meninges. We then address the method development perspective in the broader context of other tissue pathologies. Finally, we account for recent progress in artificial intelligence applications for SHG microscopy data analysis.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Second harmonic generation for brain imaging: pathology-related studies	ru_RU
dc.type	Articles in international journals and collections	ru_RU