KFU. Card publication. Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids

KAZAN
FEDERAL UNIVERSITY

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- 中文
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FABRICATION AND ATOMIC FORCE MICROSCOPY-BASED MECHANICAL ANALYSIS OF HEPATIC ORGANOIDS

Form of presentation

Articles in Russian journals and collections

Year of publication

2024

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Authors, employees of KFU

Akhatova Farida Serikovna, author

Batasheva Svetlana Nikolaevna, author

Guryanov Ivan Dmitrievich, author

Zakirova Elena Yurevna, author

Naumenko Ekaterina Anatolevna, author

Bibliographic description in the original language

Naumenko E. A. Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids / E. A. Naumenko, F. S. Akhatova, I. D. Guryanov [et al.] // Opera Medica et Physiologica. – 2024. – Vol. 11, No. 4. – P. 71-79. – DOI 10.24412/2500-2295-2024-4-71-79.

Annotation

Opera Medica et Physiologica

Keywords

HEPATIC ORGANOIDS, ATOMIC FORCE MICROSCOPY, FIBROSIS, EXTRACELLULAR MATRIX, MECHANICAL PROPERTIES

The name of the journal

Opera Medica et Physiologica

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=315892&p_lang=2

Resource files

File name	Size (MB)	Format
08_Naumenko_71_79.pdf	0,39	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Akhatova Farida Serikovna	ru_RU
dc.contributor.author	Batasheva Svetlana Nikolaevna	ru_RU
dc.contributor.author	Guryanov Ivan Dmitrievich	ru_RU
dc.contributor.author	Zakirova Elena Yurevna	ru_RU
dc.contributor.author	Naumenko Ekaterina Anatolevna	ru_RU
dc.date.accessioned	2024-01-01T00:00:00Z	ru_RU
dc.date.available	2024-01-01T00:00:00Z	ru_RU
dc.date.issued	2024	ru_RU
dc.identifier.citation	Naumenko E. A. Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids / E. A. Naumenko, F. S. Akhatova, I. D. Guryanov [et al.] // Opera Medica et Physiologica. – 2024. – Vol. 11, No. 4. – P. 71-79. – DOI 10.24412/2500-2295-2024-4-71-79.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=315892&p_lang=2	ru_RU
dc.description.abstract	Opera Medica et Physiologica	ru_RU
dc.description.abstract	The liver is a vital organ involved in a wide range of processes such as detoxification, protein synthesis, metabolism, and hormone production. Liver diseases, both inherited and viral hepatitis, liver cancer and fatty degeneration are among the leading causes of death in the world. Recent advances in 3D cell culture technology include the use of pluripotent stem cells and adult stem cells that are cultured in vitro to form self-organizing systems. Organoids are self-organizing multicellular structures that reproduce the structure and function of organs and can be used to model the development, maintenance and repair of organs ex vivo. That is why the search for new methods for the formation of hepatic organoids seen as an urgent task. For the fabrication of 3D organoids we isolated hepatic duct cells from rat liver. The obtained cellular structures were analyzed using atomic force microscopy to estimate their mechanical properties and demonstrated increasing of Young Modulus in comparison with normal liver sections. Morphometric evaluation showed that extracellular matrix fibers occupy up to 60% of the organoid, while cell agglomerations up to 40% of it. We observed the spontaneously formed fibrotic liver tissue-like constructs within 21 days. So, obtained hepatic organoids characterized by a tissue-like structure with a predominance of extracellular matrix fibers in its composition similar to liver tissue affected by fibrosis.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	HEPATIC ORGANOIDS	ru_RU
dc.subject	ATOMIC FORCE MICROSCOPY	ru_RU
dc.subject	FIBROSIS	ru_RU
dc.subject	EXTRACELLULAR MATRIX	ru_RU
dc.subject	MECHANICAL PROPERTIES	ru_RU
dc.title	Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU