KFU. Card publication. Effects of phenotypic variation on biological properties of endophytic bacteria Bacillus mojavensis PS17

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EFFECTS OF PHENOTYPIC VARIATION ON BIOLOGICAL PROPERTIES OF ENDOPHYTIC BACTERIA BACILLUS MOJAVENSIS PS17

Form of presentation

Articles in international journals and collections

Year of publication

2022

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Authors, employees of KFU

Daminova Amina Galeevna, author

Bibliographic description in the original language

Diabankana R.G.K. Effects of phenotypic variation on biological properties of endophytic bacteria Bacillus mojavensis PS17 / R. G. K. Diabankana, S. Z. Validov, A. B. Vyshtakalyuk, A. Daminova, R. I. Safin, D. M. Afordoanyi // Biology. - 2022. - V. 11. - P. 1-22.

Annotation

The use of microorganism-based products in agricultural practices is gaining more interest as an alternative to chemical methods due to their non-toxic bactericidal and fungicidal properties. Various factors influence the efficacy of the microorganisms used as biological control agents in infield conditions as compared to laboratory conditions due to ecological and physiological aspects. Abiotic factors have been shown to trigger phase variations in bacterial microorganisms as a mechanism for adapting to hostile environments. In this study, we investigated the stability of the morphotype and the effects of phenotypic variation on the biological properties of Bacillus mojavensis strain PS17. B. mojavensis PS17 generated two variants (opaque and translucent) that were given the names morphotype I and II, respectively. The partial sequence of the 16S rRNA gene revealed that both morphotypes belonged to B. mojavensis. BOX and ERIC fingerprinting PCR also showed the same DNA profiles in both morphotypes. The characteristics of morphotype I did not differ from the original strain, while morphotype II showed a lower hydrolytic enzyme activity, phytohormone production, and antagonistic ability against phytopathogenic fungi. Both morphotypes demonstrated endophytic ability in tomato plants. A low growth rate of the strain PS17(II) in a minimal medium was observed in comparison to the PS17(I) strain. Furthermore, the capacity for biocontrol of B. mojavensis PS17(II) was not effective in the suppression of root rot disease in the tomato plants caused by Fusarium oxysporum f. sp. radices-lycopersici stain ZUM2407, compared to B. mojavensis PS17(I), whose inhibition was almost 47.9 ? 1.03% effective.

Keywords

endophyte bacteria, phenotypic variation, plant colonization, B. mojavensis, antagonistic activity, biological control

The name of the journal

Biology

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=310719&p_lang=2

Resource files

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biology_11_01305_v2.pdf	8,36	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Daminova Amina Galeevna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Diabankana R.G.K. Effects of phenotypic variation on biological properties of endophytic bacteria Bacillus mojavensis PS17 / R. G. K. Diabankana, S. Z. Validov, A. B. Vyshtakalyuk, A. Daminova, R. I. Safin, D. M. Afordoanyi // Biology. - 2022. - V. 11. - P. 1-22.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=310719&p_lang=2	ru_RU
dc.description.abstract	Biology	ru_RU
dc.description.abstract	The use of microorganism-based products in agricultural practices is gaining more interest as an alternative to chemical methods due to their non-toxic bactericidal and fungicidal properties. Various factors influence the efficacy of the microorganisms used as biological control agents in infield conditions as compared to laboratory conditions due to ecological and physiological aspects. Abiotic factors have been shown to trigger phase variations in bacterial microorganisms as a mechanism for adapting to hostile environments. In this study, we investigated the stability of the morphotype and the effects of phenotypic variation on the biological properties of Bacillus mojavensis strain PS17. B. mojavensis PS17 generated two variants (opaque and translucent) that were given the names morphotype I and II, respectively. The partial sequence of the 16S rRNA gene revealed that both morphotypes belonged to B. mojavensis. BOX and ERIC fingerprinting PCR also showed the same DNA profiles in both morphotypes. The characteristics of morphotype I did not differ from the original strain, while morphotype II showed a lower hydrolytic enzyme activity, phytohormone production, and antagonistic ability against phytopathogenic fungi. Both morphotypes demonstrated endophytic ability in tomato plants. A low growth rate of the strain PS17(II) in a minimal medium was observed in comparison to the PS17(I) strain. Furthermore, the capacity for biocontrol of B. mojavensis PS17(II) was not effective in the suppression of root rot disease in the tomato plants caused by Fusarium oxysporum f. sp. radices-lycopersici stain ZUM2407, compared to B. mojavensis PS17(I), whose inhibition was almost 47.9 ? 1.03% effective.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	endophyte bacteria	ru_RU
dc.subject	phenotypic variation	ru_RU
dc.subject	plant colonization	ru_RU
dc.subject	B. mojavensis	ru_RU
dc.subject	antagonistic activity	ru_RU
dc.subject	biological control	ru_RU
dc.title	Effects of phenotypic variation on biological properties of endophytic bacteria Bacillus mojavensis PS17	ru_RU
dc.type	Articles in international journals and collections	ru_RU