KFU. Card publication. Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

EFFECT OF MELANIZATION ON THALLUS MICROSTRUCTURE IN THE LICHEN LOBARIA PULMONARIA

Form of presentation

Articles in international journals and collections

Year of publication

2022

��

��

Authors, employees of KFU

Daminova Amina Galeevna, author

Minibaeva Farida Vilevna, author

Bibliographic description in the original language

Daminova A.G., Rogov A.M., Rassabina A.E., Beckett R.P., Minibayeva F.V., Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria / A. G. Daminova ,A. M. Rogov, A. E. Rassabina , R. P. Beckett, F. V. Minibayeva // Journal of Fungi (Basel).- 2022.- V. 8. - � 791.- P. 1-10.

Annotation

Lichens often grow in microhabitats where they experience severe abiotic stresses. Some species respond to high UV radiation by synthesizing dark brown melanic pigments in the upper cortex. However, unlike the melanized structures of non-lichenized fungi, the morphology of the melanic layer in lichens remains unstudied. Here, we analyzed the morphology, ultrastructure, and elemental composition of the melanized layer in UV-exposed thalli of the lichen Lobaria pulmonaria (L.) Hoffm. Using light microscopy, we detected a pigmented layer sensitive to staining with 3,4-Ldihydroxyphenylalanine, a precursor of eumelanin, in the upper cortex of melanized thalli. Analysis of cross-sections of melanized thalli using scanning electron microscopy revealed that melanin-like granules are deposited into the hyphal lumens. Melanized thalli also possessed thicker hyphal cell walls compared to pale thalli. Energy-dispersive X-ray spectroscopy analysis of the elemental composition of the hyphal walls and extracted melanin indicated that the type of melanin synthesized by L. pulmonaria is eumelanin. Transmission electron microscopy was used to show that during melanosome-like dark vesicles are transported to the cell surface and secreted into the cell walls of the fungal hyphae. Results from this study provide new insights into the effects of melanin synthesis on the microstructure of lichen thalli.

Keywords

lichens, melanins, mycobiont, scanning electron microscopy, transmission electron microscopy, UV stress

The name of the journal

Journal of Fungi

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=310647&p_lang=2

Resource files

File name	Size (MB)	Format
F_jof_08_00791__2_.pdf	2,95	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Daminova Amina Galeevna	ru_RU
dc.contributor.author	Minibaeva Farida Vilevna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Daminova A.G., Rogov A.M., Rassabina A.E., Beckett R.P., Minibayeva F.V., Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria / A. G. Daminova ,A. M. Rogov, A. E. Rassabina , R. P. Beckett, F. V. Minibayeva // Journal of Fungi (Basel).- 2022.- V. 8. - � 791.- P. 1-10.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=310647&p_lang=2	ru_RU
dc.description.abstract	Journal of Fungi	ru_RU
dc.description.abstract	Lichens often grow in microhabitats where they experience severe abiotic stresses. Some species respond to high UV radiation by synthesizing dark brown melanic pigments in the upper cortex. However, unlike the melanized structures of non-lichenized fungi, the morphology of the melanic layer in lichens remains unstudied. Here, we analyzed the morphology, ultrastructure, and elemental composition of the melanized layer in UV-exposed thalli of the lichen Lobaria pulmonaria (L.) Hoffm. Using light microscopy, we detected a pigmented layer sensitive to staining with 3,4-Ldihydroxyphenylalanine, a precursor of eumelanin, in the upper cortex of melanized thalli. Analysis of cross-sections of melanized thalli using scanning electron microscopy revealed that melanin-like granules are deposited into the hyphal lumens. Melanized thalli also possessed thicker hyphal cell walls compared to pale thalli. Energy-dispersive X-ray spectroscopy analysis of the elemental composition of the hyphal walls and extracted melanin indicated that the type of melanin synthesized by L. pulmonaria is eumelanin. Transmission electron microscopy was used to show that during melanosome-like dark vesicles are transported to the cell surface and secreted into the cell walls of the fungal hyphae. Results from this study provide new insights into the effects of melanin synthesis on the microstructure of lichen thalli.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	lichens	ru_RU
dc.subject	melanins	ru_RU
dc.subject	mycobiont	ru_RU
dc.subject	scanning electron microscopy	ru_RU
dc.subject	transmission electron microscopy	ru_RU
dc.subject	UV stress	ru_RU
dc.title	Effect of melanization on thallus microstructure in the lichen Lobaria pulmonaria	ru_RU
dc.type	Articles in international journals and collections	ru_RU