KFU. Card publication. Mechanisms related to carbon nanotubes genotoxicity in human cell lines of respiratory origin

KAZAN
FEDERAL UNIVERSITY

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MECHANISMS RELATED TO CARBON NANOTUBES GENOTOXICITY IN HUMAN CELL LINES OF RESPIRATORY ORIGIN

Form of presentation

Articles in international journals and collections

Year of publication

2024

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Authors, employees of KFU

Daminova Amina Galeevna, author

Minibaeva Farida Vilevna, author

Bibliographic description in the original language

Fatkhutdinova L. M. Mechanisms related to carbon nanotubes genotoxicity in human cell lines of respiratory origin / L. M. Fatkhutdinova, G. F. Gabidinova, A. G. Daminova, A. M. Dimiev, T. L. Khamidullin, E. V. Valeeva, A. E. E. Cokou, S. Z. Validov, G. A. Timerbulatova // Toxicology and Applied Pharmacology. - 2024. - V. 482. - � 116784. - P. 1-16.

Annotation

Potential genotoxicity and carcinogenicity of carbon nanotubes (CNT), as well as the underlying mechanisms, remains a pressing topic. The study aimed to evaluate and compare the genotoxic effect and mechanisms of DNA damage under exposure to different types of CNT. μ Immortalized human cell lines of respiratory origin BEAS-2B, A549, MRC5-SV40 were exposed to three types of CNT: MWCNT Taunit-M, pristine and purified SWCNT TUBALL? at concentrations in the range of 0.0006–200 g/ml. Data on the CNT content in the workplace air were used to calculate the lower concentration limit. The genotoxic potential of CNTs was investigated at non-cytotoxic concentrations using a DNA comet assay. We explored reactive oxygen species (ROS) formation, direct genetic material damage, and expression of a profibrotic factor TGFB1 as mechanisms related to genotoxicity upon CNT exposure. An increase in the number of unstable DNA regions was observed at a subtoxic concentration of CNT (20 μ g/ ml), with no genotoxic effects at concentrations corresponding to industrial exposures being found. While the three test articles of CNTs exhibited comparable genotoxic potential, their mechanisms appeared to differ. MWCNTs were found to penetrate the nucleus of respiratory cells, potentially interacting directly with genetic material, as well as to enhance ROS production and TGFB1 gene expression. For A549 and MRC5-SV40, genotoxicity depended mainly on MWCNT concentration, while for BEAS-2B – on ROS production. Mechanisms of SWCNT genotoxicity were not so obvious. Oxidative stress and increased expression of profibrotic factors could not fully explain DNA damage under SWCNT exposure, and other mechanisms might be involved.

Keywords

Carbon nanotubes, Human cell lines of respiratory origin, DNA comet assay, Nuclear translocation, Oxidative stress, Transmission electron microscopy

The name of the journal

Toxicology and Applied Pharmacology

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=310632&p_lang=2

Resource files

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F_1_s2.0_S0041008X23004234_main.pdf	6,69	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Daminova Amina Galeevna	ru_RU
dc.contributor.author	Minibaeva Farida Vilevna	ru_RU
dc.date.accessioned	2024-01-01T00:00:00Z	ru_RU
dc.date.available	2024-01-01T00:00:00Z	ru_RU
dc.date.issued	2024	ru_RU
dc.identifier.citation	Fatkhutdinova L. M. Mechanisms related to carbon nanotubes genotoxicity in human cell lines of respiratory origin / L. M. Fatkhutdinova, G. F. Gabidinova, A. G. Daminova, A. M. Dimiev, T. L. Khamidullin, E. V. Valeeva, A. E. E. Cokou, S. Z. Validov, G. A. Timerbulatova // Toxicology and Applied Pharmacology. - 2024. - V. 482. - � 116784. - P. 1-16.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=310632&p_lang=2	ru_RU
dc.description.abstract	Toxicology and Applied Pharmacology	ru_RU
dc.description.abstract	Potential genotoxicity and carcinogenicity of carbon nanotubes (CNT), as well as the underlying mechanisms, remains a pressing topic. The study aimed to evaluate and compare the genotoxic effect and mechanisms of DNA damage under exposure to different types of CNT. μ Immortalized human cell lines of respiratory origin BEAS-2B, A549, MRC5-SV40 were exposed to three types of CNT: MWCNT Taunit-M, pristine and purified SWCNT TUBALL? at concentrations in the range of 0.0006–200 g/ml. Data on the CNT content in the workplace air were used to calculate the lower concentration limit. The genotoxic potential of CNTs was investigated at non-cytotoxic concentrations using a DNA comet assay. We explored reactive oxygen species (ROS) formation, direct genetic material damage, and expression of a profibrotic factor TGFB1 as mechanisms related to genotoxicity upon CNT exposure. An increase in the number of unstable DNA regions was observed at a subtoxic concentration of CNT (20 μ g/ ml), with no genotoxic effects at concentrations corresponding to industrial exposures being found. While the three test articles of CNTs exhibited comparable genotoxic potential, their mechanisms appeared to differ. MWCNTs were found to penetrate the nucleus of respiratory cells, potentially interacting directly with genetic material, as well as to enhance ROS production and TGFB1 gene expression. For A549 and MRC5-SV40, genotoxicity depended mainly on MWCNT concentration, while for BEAS-2B – on ROS production. Mechanisms of SWCNT genotoxicity were not so obvious. Oxidative stress and increased expression of profibrotic factors could not fully explain DNA damage under SWCNT exposure, and other mechanisms might be involved.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Carbon nanotubes	ru_RU
dc.subject	Human cell lines of respiratory origin	ru_RU
dc.subject	DNA comet assay	ru_RU
dc.subject	Nuclear translocation	ru_RU
dc.subject	Oxidative stress	ru_RU
dc.subject	Transmission electron microscopy	ru_RU
dc.title	Mechanisms related to carbon nanotubes genotoxicity in human cell lines of respiratory origin	ru_RU
dc.type	Articles in international journals and collections	ru_RU