KFU. Card publication. The Antimicrobial Potential of the Hop (Humulus lupulus L.) Extract against Staphylococcus aureus and Oral Streptococci

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THE ANTIMICROBIAL POTENTIAL OF THE HOP (HUMULUS LUPULUS L.) EXTRACT AGAINST STAPHYLOCOCCUS AUREUS AND ORAL STREPTOCOCCI

Form of presentation

Articles in international journals and collections

Year of publication

2024

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Authors, employees of KFU

Kayumov Ayrat Rashitovich, author

Kolesnikova Alyona Igorevna, author

Morgackaya Olga Vladimirovna, author

Trizna Elena Yurevna, author

Khayrullina Leysan Ayratovna, author

Shakirova Dilyara Khabilevna, author

Bibliographic description in the original language

Khaliullina, A.; Kolesnikova, A.; Khairullina, L.; Morgatskaya, O.; Shakirova, D.; Patov, S.; Nekrasova, P.; Bogachev, M.; Kurkin, V.; Trizna, E.; et al. The Antimicrobial Potential of the Hop (Humulus lupulus L.) Extract against Staphylococcus aureus and Oral Streptococci. Pharmaceuticals 2024, 17, 162. https://doi.org/10.3390/ph17020162

Annotation

Plant extracts are in the focus of the pharmaceutical industry as potential antimicrobials for oral care due to their high antimicrobial activity coupled with low production costs and safety for eukaryotic cells. Here, we show that the extract from Hop (Humulus lupulus L.) exhibits antimicrobial activity against Staphylococcus aureus and Streptococci in both planktonic and biofilm-embedded forms. An extract was prepared by acetone extraction from hop infructescences, followed by purification and solubilization of the remaining fraction in ethanol. The effect of the extract on S. aureus (MSSA and MRSA) was comparable with the reference antibiotics (amikacin, ciprofloxacin, and ceftriaxone) and did not depend on the bacterial resistance to methicillin. The extract also demonstrated synergy with amikacin on six S. aureus clinical isolates, on four of six isolates with ciprofloxacin, and on three of six isolates with ceftriaxone. On various Streptococci, while demonstrating lower antimicrobial activity, an extract exhibited a considerable synergistic effect in combination with two of three of these antibiotics, decreasing their MIC up to 512-fold. Moreover, the extract was able to penetrate S. aureus and S. mutans biofilms, leading to almost complete bacterial death within them. The thin-layer chromatography and LC-MS of the extract revealed the presence of prenylated flavonoids (2',4',6',4-tetrahydroxy-3'-geranylchalcone) and acylphloroglucides (cohumulone, colupulone, humulone, and lupulone), apparently responsible for the observed antimicrobial activity and ability to increase the efficiency of antibiotics. Taken together, these data suggest an extract from H. lupulus as a promising antimicrobial agent for use both as a solely antiseptic and to potentiate conventional antimicrobials.

Keywords

oral pathogens; antimicrobial resistance; antimicrobials; phytoextracts; synergy

The name of the journal

Pharmaceuticals

URL

https://www.mdpi.com/1424-8247/17/2/162

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=298479&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Kolesnikova Alyona Igorevna	ru_RU
dc.contributor.author	Morgackaya Olga Vladimirovna	ru_RU
dc.contributor.author	Trizna Elena Yurevna	ru_RU
dc.contributor.author	Khayrullina Leysan Ayratovna	ru_RU
dc.contributor.author	Shakirova Dilyara Khabilevna	ru_RU
dc.date.accessioned	2024-01-01T00:00:00Z	ru_RU
dc.date.available	2024-01-01T00:00:00Z	ru_RU
dc.date.issued	2024	ru_RU
dc.identifier.citation	Khaliullina, A.; Kolesnikova, A.; Khairullina, L.; Morgatskaya, O.; Shakirova, D.; Patov, S.; Nekrasova, P.; Bogachev, M.; Kurkin, V.; Trizna, E.; et al. The Antimicrobial Potential of the Hop (Humulus lupulus L.) Extract against Staphylococcus aureus and Oral Streptococci. Pharmaceuticals 2024, 17, 162. https://doi.org/10.3390/ph17020162	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=298479&p_lang=2	ru_RU
dc.description.abstract	Pharmaceuticals	ru_RU
dc.description.abstract	Plant extracts are in the focus of the pharmaceutical industry as potential antimicrobials for oral care due to their high antimicrobial activity coupled with low production costs and safety for eukaryotic cells. Here, we show that the extract from Hop (Humulus lupulus L.) exhibits antimicrobial activity against Staphylococcus aureus and Streptococci in both planktonic and biofilm-embedded forms. An extract was prepared by acetone extraction from hop infructescences, followed by purification and solubilization of the remaining fraction in ethanol. The effect of the extract on S. aureus (MSSA and MRSA) was comparable with the reference antibiotics (amikacin, ciprofloxacin, and ceftriaxone) and did not depend on the bacterial resistance to methicillin. The extract also demonstrated synergy with amikacin on six S. aureus clinical isolates, on four of six isolates with ciprofloxacin, and on three of six isolates with ceftriaxone. On various Streptococci, while demonstrating lower antimicrobial activity, an extract exhibited a considerable synergistic effect in combination with two of three of these antibiotics, decreasing their MIC up to 512-fold. Moreover, the extract was able to penetrate S. aureus and S. mutans biofilms, leading to almost complete bacterial death within them. The thin-layer chromatography and LC-MS of the extract revealed the presence of prenylated flavonoids (2',4',6',4-tetrahydroxy-3'-geranylchalcone) and acylphloroglucides (cohumulone, colupulone, humulone, and lupulone), apparently responsible for the observed antimicrobial activity and ability to increase the efficiency of antibiotics. Taken together, these data suggest an extract from H. lupulus as a promising antimicrobial agent for use both as a solely antiseptic and to potentiate conventional antimicrobials.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	The Antimicrobial Potential of the Hop (Humulus lupulus L.) Extract against Staphylococcus aureus and Oral Streptococci	ru_RU
dc.type	Articles in international journals and collections	ru_RU