KFU. Card publication. The Effect of Leaf Plasticity on the Isolation of Apoplastic Fluid from Leaves of Tartary Buckwheat Plants Grown In Vivo and In Vitro. Plants

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THE EFFECT OF LEAF PLASTICITY ON THE ISOLATION OF APOPLASTIC FLUID FROM LEAVES OF TARTARY BUCKWHEAT PLANTS GROWN IN VIVO AND IN VITRO. PLANTS

Form of presentation

Articles in international journals and collections

Year of publication

2023

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Authors, employees of KFU

Rumyanceva Natalya Ivanovna, author

Other authors

Ageeva Marina Vyacheslavovna, author

Valieva Alfiya Ismagilevna, author

Kostyukova Yuliya Anatolevna, author

Bibliographic description in the original language

Rumyantseva, N.I.; Valieva, A.I.; Kostyukova, Y.A.; Ageeva, M.V. The Effect of Leaf Plasticity on the Isolation of Apoplastic Fluid from Leaves of Tartary Buckwheat Plants Grown In Vivo and In Vitro. Plants 2023, 12, 4048. https://doi.org/10.3390/plants12234048

Annotation

Vacuum infiltration–centrifugation (VIC) is the most reproducible technique for the isolation of apoplast washing fluid (AWF) from leaves, but its effectiveness depends on the infiltration–centrifugation conditions and the anatomical and physiological peculiarities of leaves. This study aimed to elaborate an optimal procedure for AWF isolation from the leaves of Tartary buckwheat grown in in vivo and in vitro conditions and reveal the leaf anatomical and physiological traits that could contribute to the effectiveness of AWF isolation. Here, it was demonstrated that leaves of buckwheat plants grown in vitro could be easier infiltrated, were less sensitive to higher forces of centrifugation (900× g and 1500× g), and produced more AWF yield and apoplastic protein content than in vivo leaves at the same forces of centrifugation (600× g and 900× g). The extensive study of the morphological, anatomical, and ultrastructural characteristics of buckwheat leaves grown in different conditions revealed that in vitro leaves exhibited significant plasticity in a number of interconnected morphological, anatomical, and physiological features, generally driven by high RH and low lighting; some of them, such as the reduced thickness and increased permeability of the cuticle of the epidermal cells, large intercellular spaces, increase in the size of stomata and in the area of stomatal pores, higher stomata index, drop in density, and area of calcium oxalate druses, are beneficial to the effectiveness of VIC. The size of stomata pores, which were almost twice as large in in vitro leaves as those in in vivo ones, was the main factor contributing to the isolation of AWF free of chlorophyll contamination. The opening of stomata pores by artificially created humid conditions reduced damage to the in vivo leaves and improved the VIC of them. For Fagopyrum species, this is the first study to develop a VIC technique for AWF isolation from leaves.

Keywords

Fagopyrum tataricum; micropropagated plants; apoplast washing fluid; low lighting; high relative humidity; leaf traits; stomata; cuticle; phenotypical plasticity; adaptation

The name of the journal

Plants

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=296398&p_lang=2

Resource files

File name	Size (MB)	Format
plants_12_04048_v3__4_.pdf	8,20	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Rumyanceva Natalya Ivanovna	ru_RU
dc.contributor.author	Ageeva Marina Vyacheslavovna	ru_RU
dc.contributor.author	Valieva Alfiya Ismagilevna	ru_RU
dc.contributor.author	Kostyukova Yuliya Anatolevna	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Rumyantseva, N.I.; Valieva, A.I.; Kostyukova, Y.A.; Ageeva, M.V. The Effect of Leaf Plasticity on the Isolation of Apoplastic Fluid from Leaves of Tartary Buckwheat Plants Grown In Vivo and In Vitro. Plants 2023, 12, 4048. https://doi.org/10.3390/plants12234048	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=296398&p_lang=2	ru_RU
dc.description.abstract	Plants	ru_RU
dc.description.abstract	Vacuum infiltration–centrifugation (VIC) is the most reproducible technique for the isolation of apoplast washing fluid (AWF) from leaves, but its effectiveness depends on the infiltration–centrifugation conditions and the anatomical and physiological peculiarities of leaves. This study aimed to elaborate an optimal procedure for AWF isolation from the leaves of Tartary buckwheat grown in in vivo and in vitro conditions and reveal the leaf anatomical and physiological traits that could contribute to the effectiveness of AWF isolation. Here, it was demonstrated that leaves of buckwheat plants grown in vitro could be easier infiltrated, were less sensitive to higher forces of centrifugation (900× g and 1500× g), and produced more AWF yield and apoplastic protein content than in vivo leaves at the same forces of centrifugation (600× g and 900× g). The extensive study of the morphological, anatomical, and ultrastructural characteristics of buckwheat leaves grown in different conditions revealed that in vitro leaves exhibited significant plasticity in a number of interconnected morphological, anatomical, and physiological features, generally driven by high RH and low lighting; some of them, such as the reduced thickness and increased permeability of the cuticle of the epidermal cells, large intercellular spaces, increase in the size of stomata and in the area of stomatal pores, higher stomata index, drop in density, and area of calcium oxalate druses, are beneficial to the effectiveness of VIC. The size of stomata pores, which were almost twice as large in in vitro leaves as those in in vivo ones, was the main factor contributing to the isolation of AWF free of chlorophyll contamination. The opening of stomata pores by artificially created humid conditions reduced damage to the in vivo leaves and improved the VIC of them. For Fagopyrum species, this is the first study to develop a VIC technique for AWF isolation from leaves.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	The Effect of Leaf Plasticity on the Isolation of Apoplastic Fluid from Leaves of Tartary Buckwheat Plants Grown In Vivo and In Vitro. Plants	ru_RU
dc.type	Articles in international journals and collections	ru_RU