KFU. Card publication. Bacterial Biodiversity of the Kapova Karst Cave as a Source of HydrolasesProducers

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FEDERAL UNIVERSITY

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BACTERIAL BIODIVERSITY OF THE KAPOVA KARST CAVE AS A SOURCE OF HYDROLASESPRODUCERS

Form of presentation

Articles in international journals and collections

Year of publication

2023

Authors, employees of KFU

Ilinskaya Olga Nikolaevna, author

Kurdi Uilyam , author

Yakovleva Galina Yurevna, author

Authors, postgraduates KFU

Kurdi Uilyam , postgraduate kfu

Bibliographic description in the original language

Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave (Shulgan?Tash Nature Reserve, Bashkortostan), and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate (RNA, casein, starch).

Annotation

European Journal of Biology

Keywords

Karst cave, biodiversity, cultivated bacteria, RNase, protease, amylase

Place of publication

Istanbul

The name of the journal

European Journal of Biology

Publishing house

Istanbul university press

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=295428&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ilinskaya Olga Nikolaevna	ru_RU
dc.contributor.author	Kurdi Uilyam	ru_RU
dc.contributor.author	Yakovleva Galina Yurevna	ru_RU
dc.contributor.author	Kurdi Uilyam	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave (Shulgan?Tash Nature Reserve, Bashkortostan), and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate (RNA, casein, starch).	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=295428&p_lang=2	ru_RU
dc.description.abstract	European Journal of Biology	ru_RU
dc.language.iso	ru	ru_RU
dc.publisher	Istanbul university press	ru_RU
dc.subject	Karst cave	ru_RU
dc.subject	biodiversity	ru_RU
dc.subject	cultivated bacteria	ru_RU
dc.subject	RNase	ru_RU
dc.subject	protease	ru_RU
dc.subject	amylase	ru_RU
dc.title	Bacterial Biodiversity of the Kapova Karst Cave as a Source of HydrolasesProducers	ru_RU
dc.type	Articles in international journals and collections	ru_RU