KFU. Card publication. Influence of the Antibiotic Oxytetracycline on the Morphometric Characteristics and Endophytic Bacterial Community of Lettuce (Lactuca sativa L.)

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INFLUENCE OF THE ANTIBIOTIC OXYTETRACYCLINE ON THE MORPHOMETRIC CHARACTERISTICS AND ENDOPHYTIC BACTERIAL COMMUNITY OF LETTUCE (LACTUCA SATIVA L.)

Form of presentation

Articles in international journals and collections

Year of publication

2023

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Authors, employees of KFU

Galieva Gulnaz Shaykhinurovna, author

Galickaya Polina Yurevna, author

Danilova Natalya Viktorovna, author

Kurynceva Polina Aleksandrovna, author

Selivanovskaya Svetlana Yurevna, author

Bibliographic description in the original language

Influence of the Antibiotic Oxytetracycline on the Morphometric Characteristics and Endophytic Bacterial Community of Lettuce (Lactuca sativa L.) / Natalia Danilova, Gulnaz Galieva, Polina Kuryntseva, Svetlana Selivanovskaya, Polina Galitskaya. // Microorganisms. - 2023. - �

Annotation

Antibiotics enter the soil with compost prepared from livestock manures and other sources. There is concern that they may influence plant growth and cause antibiotic resistance in soil and plant endospheric microbiomes. In the present work, lettuce plants were cultivated in soil and hydroponics spiked with oxytetracycline (0, 15, and 300 mg × kg−1 and 0, 15, and 50 mg × L–1, respectively) during a 28-day greenhouse experiment. It was revealed that the antibiotic reduced the chlorophyll content, the biomass, and the length of the roots and stems by 1.4–4.7, 1.8–39, 2.5–3.2, and 1.8–6.3 times in soil and in hydroponics. The copy numbers of the tet(A) and tet(X) genes were revealed to be 4.51 × 103–1.58 × 105 and 8.36 × 106–1.07 × 108 copies × g–1, respectively, suggesting the potential migration of these genes from soil/hydroponics to plant roots and leaves. According to a non-metric multidimensional scaling (NMDS) analysis of the 16S rRNA amplicon sequencing data, endospheric bacterial communities were similar in leaves and roots independent of the growing substrate and antibiotic concentration. While soil bacterial communities were unaffected by the presence of antibiotics, hydroponic communities exhibited dependency, likely attributable to the absence of the mitigating effect of soil particle absorption.

Keywords

antibiotics; antibiotic-resistant genes; endophytic bacteria; plant resistome; vegetable production; hydroponic cultivation

The name of the journal

Microorganisms

URL

https://www.mdpi.com/2076-2607/11/12/2828

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=294103&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Galieva Gulnaz Shaykhinurovna	ru_RU
dc.contributor.author	Galickaya Polina Yurevna	ru_RU
dc.contributor.author	Danilova Natalya Viktorovna	ru_RU
dc.contributor.author	Kurynceva Polina Aleksandrovna	ru_RU
dc.contributor.author	Selivanovskaya Svetlana Yurevna	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Influence of the Antibiotic Oxytetracycline on the Morphometric Characteristics and Endophytic Bacterial Community of Lettuce (Lactuca sativa L.) / Natalia Danilova, Gulnaz Galieva, Polina Kuryntseva, Svetlana Selivanovskaya, Polina Galitskaya. // Microorganisms. - 2023. - �	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=294103&p_lang=2	ru_RU
dc.description.abstract	Microorganisms	ru_RU
dc.description.abstract	Antibiotics enter the soil with compost prepared from livestock manures and other sources. There is concern that they may influence plant growth and cause antibiotic resistance in soil and plant endospheric microbiomes. In the present work, lettuce plants were cultivated in soil and hydroponics spiked with oxytetracycline (0, 15, and 300 mg × kg−1 and 0, 15, and 50 mg × L–1, respectively) during a 28-day greenhouse experiment. It was revealed that the antibiotic reduced the chlorophyll content, the biomass, and the length of the roots and stems by 1.4–4.7, 1.8–39, 2.5–3.2, and 1.8–6.3 times in soil and in hydroponics. The copy numbers of the tet(A) and tet(X) genes were revealed to be 4.51 × 103–1.58 × 105 and 8.36 × 106–1.07 × 108 copies × g–1, respectively, suggesting the potential migration of these genes from soil/hydroponics to plant roots and leaves. According to a non-metric multidimensional scaling (NMDS) analysis of the 16S rRNA amplicon sequencing data, endospheric bacterial communities were similar in leaves and roots independent of the growing substrate and antibiotic concentration. While soil bacterial communities were unaffected by the presence of antibiotics, hydroponic communities exhibited dependency, likely attributable to the absence of the mitigating effect of soil particle absorption.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Influence of the Antibiotic Oxytetracycline on the Morphometric Characteristics and Endophytic Bacterial Community of Lettuce (Lactuca sativa L.)	ru_RU
dc.type	Articles in international journals and collections	ru_RU