KFU. Card publication. Efficient delivery of the immunodomi-nant genes of African swine fever virus by adeno-associated virus serotype 2

KAZAN
FEDERAL UNIVERSITY

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EFFICIENT DELIVERY OF THE IMMUNODOMI-NANT GENES OF AFRICAN SWINE FEVER VIRUS BY ADENO-ASSOCIATED VIRUS SEROTYPE 2

Form of presentation

Articles in international journals and collections

Year of publication

2023

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Authors, employees of KFU

Zakirova Elena Yurevna, author

Rizvanov Albert Anatolevich, author

Other authors

Ravilov Rustem , author

Bibliographic description in the original language

Ravilov R. Efficient delivery of the immunodominant genes of African swine fever virus by adeno-associated virus serotype 2 / Ravilov R, Galeeva A, Frolov G, Efimova M, Zakirova E, Rizvanov A, Hisamutdinov A, Garipov L, and Mingaleev D // Veterinary World. - 2023. - T.16. - �12. - r.2425-2430.

Annotation

Veterinary World

Keywords

adeno-associated virus, African swine fever virus, gene delivery, virally vectored vaccine

The name of the journal

Veterinary World

URL

https://www.researchgate.net/publication/376290676_Efficient_delivery_of_the_immunodominant_genes_of_African_swine_fever_virus_by_adeno-associated_virus_serotype_2

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=293165&p_lang=2

Resource files

File name	Size (MB)	Format
5.pdf	0,86	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Zakirova Elena Yurevna	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Ravilov Rustem	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Ravilov R. Efficient delivery of the immunodominant genes of African swine fever virus by adeno-associated virus serotype 2 / Ravilov R, Galeeva A, Frolov G, Efimova M, Zakirova E, Rizvanov A, Hisamutdinov A, Garipov L, and Mingaleev D // Veterinary World. - 2023. - �.16. - �12. - �.2425-2430.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=293165&p_lang=2	ru_RU
dc.description.abstract	Veterinary World	ru_RU
dc.description.abstract	Adeno-associated virus serotype 2 (AAV2) represents a promising basis for developing a virus-vector vaccine against African swine fever (ASF). This study aimed to create genetic constructs based on AAV2 to deliver the immunodominant genes of ASF virus (ASFV) and to evaluate their functionality in vitro. The efficiency and specificity of transgene expression, as well as their non-toxicity in cells of target animals, were evaluated. Materials and Methods: Bioinformatics analysis methods were used to identify the immunodominant genes of ASFV. The target genes B646L, E183L, CP204L, and CP530R were identified and subsequently cloned into the pAAV-MCS vector. Assembly of recombinant AAV2 (rAAV2) was performed by cotransfection of AAV293 cells with the following plasmids: pAAV-MCS with the gene of interest, envelope, and packaging. Quantitative polymerase chain reaction was used to determine the AAV2 titer. The functionality of the constructs was evaluated in HEK293 and SPEV cells by determining the presence of mature proteins in the cell lysate and the expression levels of messenger RNA. The specificity of the target proteins in cell lysates was confirmed by Western blotting. Results: The proposed AAV2 assembly protocol makes it possible to achieve a concentration of mature viral particles of at least 280 billion/mL of virus-containing material. The rAAV2 could effectively transduce host SPEV cells. The expression of both cistrons was detectable during the transduction of cells; therefore, the combined expression of immunogens in the cells of target animals should be possible using this method. Conclusion: This study demonstrated the potential of using genetic constructs based on AAV2 for the delivery of ASFV genes in vitro. Keywords: adeno-associated virus, African swine fever virus, gene delivery, virally vectored vaccine.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	adeno-associated virus	ru_RU
dc.subject	African swine fever virus	ru_RU
dc.subject	gene delivery	ru_RU
dc.subject	virally vectored vaccine	ru_RU
dc.title	Efficient delivery of the immunodomi-nant genes of African swine fever virus by adeno-associated virus serotype 2	ru_RU
dc.type	Articles in international journals and collections	ru_RU