KFU. Card publication. The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

THE EFFECT OF FICIN IMMOBILIZED ON CARBOXYMETHYL CHITOSAN ON BIOFILMS OF ORAL PATHOGENS

Form of presentation

Articles in international journals and collections

Year of publication

2023

��

��

Authors, employees of KFU

Baydamshina Diana Rafisovna, author

Gafarova Lyaysan Faridovna, author

Zelenikhin Pavel Valerevich, author

Kayumov Ayrat Rashitovich, author

Melnik Anastasiya Pavlovna, author

Ostolopovskaya Olga Vyacheslavovna, author

Sokolova Evgeniya Aleksandrovna, author

Trizna Elena Yurevna, author

Bibliographic description in the original language

Baidamshina, D.R. The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens / D.R. Baidamshina, E.Yu. Trizna, S.S. Goncharova, A.V. Sorokin M.S. Lavlinskaya A.P. Melnik L.F. Gafarova, M.A. Kharitonova, O.V. Ostolopovskaya, V.G. Artyukhov, E.A. Sokolova, M.G. Holyavka, M.I. Bogachev, A.R. Kayumov, P.V. Zelenikhin // International Journal of Molecular Sciences – 2023. – V.24. – N 16090. https://doi.org/10.3390/ijms242216090

Annotation

In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19–32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by Streptococcus salivarius, Streptococcus gordonii, Streptococcus mutans, and Candida albicans, while no apparent effect on Staphylococcus aureus was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by Streptococcus sobrinus and S. gordonii. By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers S. gordonii and S. mutans. In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral Streptococci biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization.

Keywords

carboxymethyl chitosan; ficin; oral microbiota; bacterial biofilms

The name of the journal

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

URL

https://www.mdpi.com/1422-0067/24/22/16090

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=292119&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Baydamshina Diana Rafisovna	ru_RU
dc.contributor.author	Gafarova Lyaysan Faridovna	ru_RU
dc.contributor.author	Zelenikhin Pavel Valerevich	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Melnik Anastasiya Pavlovna	ru_RU
dc.contributor.author	Ostolopovskaya Olga Vyacheslavovna	ru_RU
dc.contributor.author	Sokolova Evgeniya Aleksandrovna	ru_RU
dc.contributor.author	Trizna Elena Yurevna	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Baidamshina, D.R. The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens / D.R. Baidamshina, E.Yu. Trizna, S.S. Goncharova, A.V. Sorokin M.S. Lavlinskaya A.P. Melnik L.F. Gafarova, M.A. Kharitonova, O.V. Ostolopovskaya, V.G. Artyukhov, E.A. Sokolova, M.G. Holyavka, M.I. Bogachev, �.R. Kayumov, P.V. Zelenikhin // International Journal of Molecular Sciences – 2023. – V.24. – N 16090. https://doi.org/10.3390/ijms242216090	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=292119&p_lang=2	ru_RU
dc.description.abstract	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES	ru_RU
dc.description.abstract	In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19–32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by Streptococcus salivarius, Streptococcus gordonii, Streptococcus mutans, and Candida albicans, while no apparent effect on Staphylococcus aureus was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by Streptococcus sobrinus and S. gordonii. By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers S. gordonii and S. mutans. In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral Streptococci biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens	ru_RU
dc.type	Articles in international journals and collections	ru_RU