KFU. Card publication. Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats

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MATERNAL HYPERHOMOCYSTEINEMIA INCREASES SEIZURES SUSCEPTIBILITY OF NEONATAL RATS

Form of presentation

Articles in international journals and collections

Year of publication

2023

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Authors, employees of KFU

Sitdikova Guzel Faritovna, author

Yakovlev Aleksey Valerevich, author

Bibliographic description in the original language

A.V. Yakovlev, E. Kurmashova, E. Gataulina, E. Gerasimova, I. Khalilov, G.F. Sitdikova, Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats, Life Sciences, Volume 329, 2023, 121953, ttps://doi.org/10.1016/j.lfs.2023.121953.

Annotation

Neonatal seizures are severe pathologies which may result in long-term neurological consequences. High plasma concentrations of homocysteine – hyperhomocysteinemia (hHCy) - are associated with epilepsy. In the present study, we evaluated susceptibility to seizure of neonatal rats with prenatal hHCy. Main methods Prenatal hHCy was induced by feeding females with a high-methionine diet. Experiments were performed on pups during the first three postnatal weeks. Flurothyl-induced epileptic behavior was assessed according to Racine's scale. Epileptiform activity in the hippocampus was recorded using electrophysiological methods. The balance of excitation/inhibition, functional GABAergic inhibition and GABA reversal potential in hippocampal neurons were analyzed. Key findings Rats with hHCy developed more severe stages of behavioral patterns during flurothyl-induced epilepsy with shorter latency. Electrophysiological recordings demonstrated higher background neuronal activity in rats with hHCy. Seizure-like events triggered by flurothyl (in vivo) or 4-aminopyridine (in vitro) showed shorter latency, higher power and amplitude. An increased glutamate/GABA synaptic ratio was shown in the pyramidal neurons of rats with hHCy and more slices demonstrated excitation by isoguvacine, a selective GABA(A) receptor agonist, during the first and second postnatal weeks. The GABA driving force and the reversal potential of GABA(A) currents were more positive during the second postnatal week for hHCy rats. Significance The higher susceptibility to seizures in rats with prenatal hHCy due to a shift in the balance of excitation/inhibition toward excitation may underlie the clinical evidence about the association of hHCy with an increased risk of epilepsy.

Keywords

EpilepsyImmature ratshippocampusHomocysteineExcitation/inhibition balanceGABA driving force

The name of the journal

Life Sciences

URL

https://www.sciencedirect.com/science/article/pii/S002432052300588X

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=286718&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sitdikova Guzel Faritovna	ru_RU
dc.contributor.author	Yakovlev Aleksey Valerevich	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	A.V. Yakovlev, E. Kurmashova, E. Gataulina, E. Gerasimova, I. Khalilov, G.F. Sitdikova, Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats, Life Sciences, Volume 329, 2023, 121953, ttps://doi.org/10.1016/j.lfs.2023.121953.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=286718&p_lang=2	ru_RU
dc.description.abstract	Life Sciences	ru_RU
dc.description.abstract	Neonatal seizures are severe pathologies which may result in long-term neurological consequences. High plasma concentrations of homocysteine – hyperhomocysteinemia (hHCy) - are associated with epilepsy. In the present study, we evaluated susceptibility to seizure of neonatal rats with prenatal hHCy. Main methods Prenatal hHCy was induced by feeding females with a high-methionine diet. Experiments were performed on pups during the first three postnatal weeks. Flurothyl-induced epileptic behavior was assessed according to Racine's scale. Epileptiform activity in the hippocampus was recorded using electrophysiological methods. The balance of excitation/inhibition, functional GABAergic inhibition and GABA reversal potential in hippocampal neurons were analyzed. Key findings Rats with hHCy developed more severe stages of behavioral patterns during flurothyl-induced epilepsy with shorter latency. Electrophysiological recordings demonstrated higher background neuronal activity in rats with hHCy. Seizure-like events triggered by flurothyl (in vivo) or 4-aminopyridine (in vitro) showed shorter latency, higher power and amplitude. An increased glutamate/GABA synaptic ratio was shown in the pyramidal neurons of rats with hHCy and more slices demonstrated excitation by isoguvacine, a selective GABA(A) receptor agonist, during the first and second postnatal weeks. The GABA driving force and the reversal potential of GABA(A) currents were more positive during the second postnatal week for hHCy rats. Significance The higher susceptibility to seizures in rats with prenatal hHCy due to a shift in the balance of excitation/inhibition toward excitation may underlie the clinical evidence about the association of hHCy with an increased risk of epilepsy.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Maternal hyperhomocysteinemia increases seizures susceptibility of neonatal rats	ru_RU
dc.type	Articles in international journals and collections	ru_RU