KFU. Card publication. Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum

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INDUCED EXPRESSION OF CYP51A AND HK1 GENES ASSOCIATED WITH PENCONAZOLE AND FLUDIOXONIL RESISTANCE IN THE POTATO PATHOGEN FUSARIUM OXYSPORUM

Form of presentation

Articles in international journals and collections

Year of publication

2023

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Authors, employees of KFU

Kostennikova Zarina Sirozhidinovna, author

Lutfullin Marat Tafkilevich, author

Lutfullina Guzel Fanisovna, author

Mardanova Ayslu Mirkasymovna, author

Other authors

Afordoanyi Daniel M., author

Akosah A. Yaw, author

Vologin Semen Germanovich, author

Bibliographic description in the original language

Akosah, Y. A., Kostennikova, Z. S., Lutfullin, M. T., Lutfullina, G. F., Afordoanyi, D. M., Vologin, S. G., & Mardanova, A. M. (2023). Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum. Microorganisms, 11(5), 1257. https://doi.org/10.3390/microorganisms11051257

Annotation

Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated Fusarium oxysporum isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (CYP51a) and histidine kinase (HK1) genes. Penconazole stunted the growth of F. oxysporum strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in F. oxysporum. With an increase in the concentration of fludioxonil, only one strain (F. oxysporum S95) exhibited moderate sensitivity to the fungicide. Interaction of F. oxysporum with penconazole and fludioxonil leads to respective elevated expressions of the CYP51a and HK1 genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time.

Keywords

CYP51; Fusarium; Solanum tuberosum L.; dry rot; fludioxonil; histidine kinase; penconazole; wilt.

The name of the journal

Microorganisms

URL

https://doi.org/10.3390/microorganisms11051257

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=283877&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Kostennikova Zarina Sirozhidinovna	ru_RU
dc.contributor.author	Lutfullin Marat Tafkilevich	ru_RU
dc.contributor.author	Lutfullina Guzel Fanisovna	ru_RU
dc.contributor.author	Mardanova Ayslu Mirkasymovna	ru_RU
dc.contributor.author	Afordoanyi Daniel M.	ru_RU
dc.contributor.author	Akosah A. Yaw	ru_RU
dc.contributor.author	Vologin Semen Germanovich	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Akosah, Y. A., Kostennikova, Z. S., Lutfullin, M. T., Lutfullina, G. F., Afordoanyi, D. M., Vologin, S. G., & Mardanova, A. M. (2023). Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum. Microorganisms, 11(5), 1257. https://doi.org/10.3390/microorganisms11051257	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=283877&p_lang=2	ru_RU
dc.description.abstract	Microorganisms	ru_RU
dc.description.abstract	Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated Fusarium oxysporum isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (CYP51a) and histidine kinase (HK1) genes. Penconazole stunted the growth of F. oxysporum strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in F. oxysporum. With an increase in the concentration of fludioxonil, only one strain (F. oxysporum S95) exhibited moderate sensitivity to the fungicide. Interaction of F. oxysporum with penconazole and fludioxonil leads to respective elevated expressions of the CYP51a and HK1 genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum	ru_RU
dc.type	Articles in international journals and collections	ru_RU