Form of presentation | Articles in international journals and collections |
Year of publication | 2023 |
Язык | английский |
|
Kostennikova Zarina Sirozhidinovna, author
Lutfullin Marat Tafkilevich, author
Lutfullina Guzel Fanisovna, author
Mardanova Ayslu Mirkasymovna, author
|
|
Afordoanyi Daniel M., author
Akosah A. Yaw, author
Vologin Semen Germanovich, author
|
Bibliographic description in the original language |
Akosah, Y. A., Kostennikova, Z. S., Lutfullin, M. T., Lutfullina, G. F., Afordoanyi, D. M., Vologin, S. G., & Mardanova, A. M. (2023). Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum. Microorganisms, 11(5), 1257. https://doi.org/10.3390/microorganisms11051257 |
Annotation |
Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated Fusarium oxysporum isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (CYP51a) and histidine kinase (HK1) genes. Penconazole stunted the growth of F. oxysporum strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in F. oxysporum. With an increase in the concentration of fludioxonil, only one strain (F. oxysporum S95) exhibited moderate sensitivity to the fungicide. Interaction of F. oxysporum with penconazole and fludioxonil leads to respective elevated expressions of the CYP51a and HK1 genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time. |
Keywords |
CYP51; Fusarium; Solanum tuberosum L.; dry rot; fludioxonil; histidine kinase; penconazole; wilt. |
The name of the journal |
Microorganisms
|
URL |
https://doi.org/10.3390/microorganisms11051257 |
Please use this ID to quote from or refer to the card |
https://repository.kpfu.ru/eng/?p_id=283877&p_lang=2 |
Full metadata record ![](https://shelly.kpfu.ru/pdf/picture/arrow_black_right.gif) |
Field DC |
Value |
Language |
dc.contributor.author |
Kostennikova Zarina Sirozhidinovna |
ru_RU |
dc.contributor.author |
Lutfullin Marat Tafkilevich |
ru_RU |
dc.contributor.author |
Lutfullina Guzel Fanisovna |
ru_RU |
dc.contributor.author |
Mardanova Ayslu Mirkasymovna |
ru_RU |
dc.contributor.author |
Afordoanyi Daniel M. |
ru_RU |
dc.contributor.author |
Akosah A. Yaw |
ru_RU |
dc.contributor.author |
Vologin Semen Germanovich |
ru_RU |
dc.date.accessioned |
2023-01-01T00:00:00Z |
ru_RU |
dc.date.available |
2023-01-01T00:00:00Z |
ru_RU |
dc.date.issued |
2023 |
ru_RU |
dc.identifier.citation |
Akosah, Y. A., Kostennikova, Z. S., Lutfullin, M. T., Lutfullina, G. F., Afordoanyi, D. M., Vologin, S. G., & Mardanova, A. M. (2023). Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum. Microorganisms, 11(5), 1257. https://doi.org/10.3390/microorganisms11051257 |
ru_RU |
dc.identifier.uri |
https://repository.kpfu.ru/eng/?p_id=283877&p_lang=2 |
ru_RU |
dc.description.abstract |
Microorganisms |
ru_RU |
dc.description.abstract |
Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated Fusarium oxysporum isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (CYP51a) and histidine kinase (HK1) genes. Penconazole stunted the growth of F. oxysporum strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in F. oxysporum. With an increase in the concentration of fludioxonil, only one strain (F. oxysporum S95) exhibited moderate sensitivity to the fungicide. Interaction of F. oxysporum with penconazole and fludioxonil leads to respective elevated expressions of the CYP51a and HK1 genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time. |
ru_RU |
dc.language.iso |
ru |
ru_RU |
dc.subject |
|
ru_RU |
dc.title |
Induced Expression of CYP51a and HK1 Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen Fusarium oxysporum |
ru_RU |
dc.type |
Articles in international journals and collections |
ru_RU |
|