KFU. Card publication. Quantitative changes in perineuronal nets in development and posttraumatic condition

KAZAN
FEDERAL UNIVERSITY

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QUANTITATIVE CHANGES IN PERINEURONAL NETS IN DEVELOPMENT AND POSTTRAUMATIC CONDITION

Form of presentation

Articles in international journals and collections

Year of publication

2019

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Authors, employees of KFU

Aganov Albert Vartanovich, author

Kiyasov Andrey Pavlovich, author

Kochneva Anastasiya Anatolevna, author

Mavlikeev Mikhail Olegovich, author

Melnikova Anastasiya Aleksandrovna, author

Bibliographic description in the original language

Lipachev, N. Quantitative changes in perineuronal nets in development and posttraumatic condition [Text] / N. Lipachev, N. Arnst, A. Melnikova, H. Jäälinoja, A. Kochneva, A. Zhigalov, N. Kulesskaya, A.V. Aganov, M. Mavlikeev, H. Rauvala, A.P. Kiyasov, M. Paveliev // Journal of Molecular Histology. – 2019. - Vol. 50, Issue 3. – P. 203–216.

Annotation

Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.

Keywords

Perineuronal nets ? Extracellular matrix ? Somatosensory cortex ? Brain development ? Spinal cord injury

The name of the journal

Journal of Molecular Histology

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=279110&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Aganov Albert Vartanovich	ru_RU
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Kochneva Anastasiya Anatolevna	ru_RU
dc.contributor.author	Mavlikeev Mikhail Olegovich	ru_RU
dc.contributor.author	Melnikova Anastasiya Aleksandrovna	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Lipachev, N. Quantitative changes in perineuronal nets in development and posttraumatic condition [Text] / N. Lipachev, N. Arnst, A. Melnikova, H. Jäälinoja, A. Kochneva, A. Zhigalov, N. Kulesskaya, A.V. Aganov, M. Mavlikeev, H. Rauvala, A.P. Kiyasov, M. Paveliev // Journal of Molecular Histology. – 2019. - Vol. 50, Issue 3. – P. 203–216.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=279110&p_lang=2	ru_RU
dc.description.abstract	Journal of Molecular Histology	ru_RU
dc.description.abstract	Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Quantitative changes in perineuronal nets in development and posttraumatic condition	ru_RU
dc.type	Articles in international journals and collections	ru_RU