KFU. Card publication. A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP

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FEDERAL UNIVERSITY

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A HIGH CALCIUM LEVEL-BASED MODEL FOR IDENTIFYING POSTSYNAPTIC EFFECTS OF ATP

Form of presentation

Articles in international journals and collections

Year of publication

2023

Язык

английский

Authors, employees of KFU

Grishin Sergey Nikolaevich, author

Eremeev Anton Aleksandrovich, author

Khayrullin Adel Evgenevich, author

Other authors

Ziganshin Ayrat Usmanovich, author

Bibliographic description in the original language

Khairullin, A.E. A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP/ A.E.Khairullin, S.N.Grishin, A.Y. Teplov, A.A. Eremeev, T.V. Baltina, A.U. Ziganshin// BIOPHYSICS. - 2022. - Vol.67. - P.1007–1010

Annotation

Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsynaptic side of purinergic signaling during the application of electrical stimulation led to the idea of controlling the presynaptic terminal of ATP-mediated modulation. In in vitro experiments, electromyograms and mechanomyograms during isometric contractions of isolated nerve-muscle preparations of rat soleus and extensor digitorum longus (EDL) muscles revealed postsynaptic effects of ATP in the presence of a high intracellular calcium level. Thus, the effects of ATP in the presence of increased Ca2+ content were seen through contraction of soleus muscles that started to contract quicker by fifty percent and inhibition of contractility of EDL muscles; this was in accord with data obtained earlier on carbachol-induced contractions. We have demonstrated an ATP-dependent processes in the postsynaptic site that may contribute significantly to adaptation mechanisms in hypothermia.

Keywords

neuromuscular synapse, hypercalcium model, ATP, suramin postsynaptic effects

The name of the journal

Biophysics

URL

https://link.springer.com/article/10.1134/S0006350922050086

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=277426&p_lang=2

Resource files

File name	Size (MB)	Format
F_S0006350922050086.pdf	0,28	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Grishin Sergey Nikolaevich	ru_RU
dc.contributor.author	Eremeev Anton Aleksandrovich	ru_RU
dc.contributor.author	Khayrullin Adel Evgenevich	ru_RU
dc.contributor.author	Ziganshin Ayrat Usmanovich	ru_RU
dc.date.accessioned	2023-01-01T00:00:00Z	ru_RU
dc.date.available	2023-01-01T00:00:00Z	ru_RU
dc.date.issued	2023	ru_RU
dc.identifier.citation	Khairullin, A.E. A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP/ A.E.Khairullin, S.N.Grishin, A.Y. Teplov, А.А. Еремеев, Т.В. Балтина, А.У. Зиганшин// BIOPHYSICS. - 2022. - Vol.67. - P.1007–1010	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=277426&p_lang=2	ru_RU
dc.description.abstract	Biophysics	ru_RU
dc.description.abstract	Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsynaptic side of purinergic signaling during the application of electrical stimulation led to the idea of controlling the presynaptic terminal of ATP-mediated modulation. In in vitro experiments, electromyograms and mechanomyograms during isometric contractions of isolated nerve-muscle preparations of rat soleus and extensor digitorum longus (EDL) muscles revealed postsynaptic effects of ATP in the presence of a high intracellular calcium level. Thus, the effects of ATP in the presence of increased Ca2+ content were seen through contraction of soleus muscles that started to contract quicker by fifty percent and inhibition of contractility of EDL muscles; this was in accord with data obtained earlier on carbachol-induced contractions. We have demonstrated an ATP-dependent processes in the postsynaptic site that may contribute significantly to adaptation mechanisms in hypothermia.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	neuromuscular synapse	ru_RU
dc.subject	hypercalcium model	ru_RU
dc.subject	ATP	ru_RU
dc.subject	suramin postsynaptic effects	ru_RU
dc.title	A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP	ru_RU
dc.type	Articles in international journals and collections	ru_RU