KFU. Card publication. Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia

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LEVELS OF PROTEIN CARBONYLATION AND ACTIVITY OF PROTEASES IN THE BRAIN OF NEWBORN RATS WITH PRENATAL HYPERHOMOCYSTEINEMIA

Form of presentation

Articles in international journals and collections

Year of publication

2022

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Authors, employees of KFU

Sitdikova Guzel Faritovna, author

Yakovlev Aleksey Valerevich, author

Yakovleva Olga Vladislavovna, author

Bibliographic description in the original language

A. V. Yakovlev, S. A. Dmitrieva, A. N. Krasnova, O. V. Yakovleva and G. F. Sitdikova Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia // Neurochemical Journal, 2022, Vol. 16, No. 3, pp. 263–270.

Annotation

Homocysteine is a sulfur-containing amino acid formed from methionine and considered as a risk factor for a number of pathologies. An increase in the homocysteine level (hyperhomocysteinemia, HHcy) during pregnancy leads to various complications of pregnancy, fetal hypoxia and, consequently, development of early and delayed postnatal pathologies. One of the main mechanisms of homocysteine action is induction of oxidative stress. The aim of our study was to analyze oxidative modification of proteins and activity of pro- teases, as well as the level of oxidative stress in the brain tissue of rats with prenatal HHcy in the first week after birth. Experimental HHcy was induced in female rats by feeding them with elevated amounts of methi- onine for 3 weeks before, during, and after pregnancy. In the homogenates of brain tissue of offspring with prenatal HHcy a significant increase in spontaneous protein carbonylation was observed. This result indicates a decrease in the reserve-adaptive potential of brain cells and a decrease in the resistance of the tissue to the action of free radicals. In the brains of rats with prenatal HHcy, the activity of acidic and neutral proteases was higher compared to controls which could be a result of aggregation and fragmentation of protein mole- cules due to carbonylation of amino acid residues. Brain tissues of newborn rats with prenatal HHcy were also characterized by a high content of hydrogen peroxide, malondialdehyde as the marker of lipid peroxidation, and a decrease in the activity of the antioxidant enzymes superoxide dismutase, catalase, glutathione perox- idase, and glutathione reductase. Thus, the data obtained indicate a significant increase in the irreversible process of oxidative modification of proteins in the brain of newborn rats with prenatal HHcy due to oxidative stress. These processes contribute to the mechanisms of homocysteine neurotoxicity during the critical period of brain development in the early postnatal period, during which intensive neurogenesis, synaptogenesis, and formation of neural networks take place.

Keywords

oxidative modification of proteins, prenatal hyperhomocysteinemia, brain, proteases, antioxidant systems, lipid peroxidation

The name of the journal

Neurochemical Journal

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=276245&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sitdikova Guzel Faritovna	ru_RU
dc.contributor.author	Yakovlev Aleksey Valerevich	ru_RU
dc.contributor.author	Yakovleva Olga Vladislavovna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	A. V. Yakovlev, S. A. Dmitrieva, A. N. Krasnova, O. V. Yakovleva and G. F. Sitdikova Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia // Neurochemical Journal, 2022, Vol. 16, No. 3, pp. 263–270.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=276245&p_lang=2	ru_RU
dc.description.abstract	Neurochemical Journal	ru_RU
dc.description.abstract	Homocysteine is a sulfur-containing amino acid formed from methionine and considered as a risk factor for a number of pathologies. An increase in the homocysteine level (hyperhomocysteinemia, HHcy) during pregnancy leads to various complications of pregnancy, fetal hypoxia and, consequently, development of early and delayed postnatal pathologies. One of the main mechanisms of homocysteine action is induction of oxidative stress. The aim of our study was to analyze oxidative modification of proteins and activity of pro- teases, as well as the level of oxidative stress in the brain tissue of rats with prenatal HHcy in the first week after birth. Experimental HHcy was induced in female rats by feeding them with elevated amounts of methi- onine for 3 weeks before, during, and after pregnancy. In the homogenates of brain tissue of offspring with prenatal HHcy a significant increase in spontaneous protein carbonylation was observed. This result indicates a decrease in the reserve-adaptive potential of brain cells and a decrease in the resistance of the tissue to the action of free radicals. In the brains of rats with prenatal HHcy, the activity of acidic and neutral proteases was higher compared to controls which could be a result of aggregation and fragmentation of protein mole- cules due to carbonylation of amino acid residues. Brain tissues of newborn rats with prenatal HHcy were also characterized by a high content of hydrogen peroxide, malondialdehyde as the marker of lipid peroxidation, and a decrease in the activity of the antioxidant enzymes superoxide dismutase, catalase, glutathione perox- idase, and glutathione reductase. Thus, the data obtained indicate a significant increase in the irreversible process of oxidative modification of proteins in the brain of newborn rats with prenatal HHcy due to oxidative stress. These processes contribute to the mechanisms of homocysteine neurotoxicity during the critical period of brain development in the early postnatal period, during which intensive neurogenesis, synaptogenesis, and formation of neural networks take place.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	oxidative modification of proteins	ru_RU
dc.subject	prenatal hyperhomocysteinemia	ru_RU
dc.subject	brain	ru_RU
dc.subject	proteases	ru_RU
dc.subject	antioxidant systems	ru_RU
dc.subject	lipid peroxidation	ru_RU
dc.title	Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia	ru_RU
dc.type	Articles in international journals and collections	ru_RU