KFU. Card publication. Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements

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ALARMING ANTIBIOTIC RESISTANCE OF LACTOBACILLI ISOLATED FROM PROBIOTIC PREPARATIONS AND DIETARY SUPPLEMENTS

Form of presentation

Articles in international journals and collections

Year of publication

2022

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Authors, employees of KFU

Yarullina Dina Rashidovna, author

Bibliographic description in the original language

Anisimova E, Gorokhova I, Karimullina G, Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements//Antibiotics. - 2022. - Vol.11, Is.11. - Art. �1557.

Annotation

In this study we screened eight commercially available brands of Lactobacillus-containing probiotic preparations and dietary supplements for resistance towards commonly administered antibiotics of different classes. According to the results of disc diffusion method, most of the isolates were resistant to vancomycin and susceptible to penicillin-type antibiotics (ampicillin and amoxicillin), carbapenems (imipenem, meropenem, and ertapenem), and inhibitors of protein synthesis (chloramphenicol, erythromycin, tetracycline, clarithromycin, and linezolid). Yet, based on the minimum inhibitory concentration (MIC) values six strains were reconsidered as resistant to tetracycline. All tested lactobacilli were resistant towards amikacin, ciprofloxacin, and norfloxacin. Resistance to cephalosporins was highly variable and decreased in a row: ceftazidime/cefepime, ceftriaxone, cefotaxime, cefazolin, and cefoperazone. PCR screening for antibiotic resistance de-terminants in probiotic lactobacilli revealed wide occurrence of vancomycin resistance gene vanX, ciprofloxacin resistance gene parC, and extended-spectrum β-lactamase gene blaTEM. We also de-tected tetK gene for tetracycline resistance in one isolate. Additionally, for several products we demonstrated discrepancies between the claims of the manufacturers and the identified species composition, as well as enumerated amount of viable bacteria. The results of this study raise concern about safety of lactobacilli for human consumption as probiotics since they may act as reservoirs of transferable antibiotic resistance genes.

Keywords

Lactobacilli, probiotics, safety, antibiotic resistance, resistance genes.

The name of the journal

Antibiotics

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85141757017&doi=10.3390%2fantibiotics11111557&partnerID=40&md5=b94d3cecdb26b2739633126496af578b

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=274286&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Yarullina Dina Rashidovna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Anisimova E, Gorokhova I, Karimullina G, Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements//Antibiotics. - 2022. - Vol.11, Is.11. - Art. �1557.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=274286&p_lang=2	ru_RU
dc.description.abstract	Antibiotics	ru_RU
dc.description.abstract	In this study we screened eight commercially available brands of Lactobacillus-containing probiotic preparations and dietary supplements for resistance towards commonly administered antibiotics of different classes. According to the results of disc diffusion method, most of the isolates were resistant to vancomycin and susceptible to penicillin-type antibiotics (ampicillin and amoxicillin), carbapenems (imipenem, meropenem, and ertapenem), and inhibitors of protein synthesis (chloramphenicol, erythromycin, tetracycline, clarithromycin, and linezolid). Yet, based on the minimum inhibitory concentration (MIC) values six strains were reconsidered as resistant to tetracycline. All tested lactobacilli were resistant towards amikacin, ciprofloxacin, and norfloxacin. Resistance to cephalosporins was highly variable and decreased in a row: ceftazidime/cefepime, ceftriaxone, cefotaxime, cefazolin, and cefoperazone. PCR screening for antibiotic resistance de-terminants in probiotic lactobacilli revealed wide occurrence of vancomycin resistance gene vanX, ciprofloxacin resistance gene parC, and extended-spectrum β-lactamase gene blaTEM. We also de-tected tetK gene for tetracycline resistance in one isolate. Additionally, for several products we demonstrated discrepancies between the claims of the manufacturers and the identified species composition, as well as enumerated amount of viable bacteria. The results of this study raise concern about safety of lactobacilli for human consumption as probiotics since they may act as reservoirs of transferable antibiotic resistance genes.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Lactobacilli	ru_RU
dc.subject	probiotics	ru_RU
dc.subject	safety	ru_RU
dc.subject	antibiotic resistance	ru_RU
dc.subject	resistance genes.	ru_RU
dc.title	Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements	ru_RU
dc.type	Articles in international journals and collections	ru_RU