KFU. Card publication. Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus

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FEDERAL UNIVERSITY

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BACKBONE AND SIDE CHAIN NMR ASSIGNMENTS FOR THE RIBOSOME MATURATION FACTOR P (RIMP) FROM STAPHYLOCOCCUS AUREUS

Form of presentation

Articles in international journals and collections

Year of publication

2022

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Authors, employees of KFU

Garaeva Nataliya Sergeevna, author

Bibliographic description in the original language

N. S. Garaeva Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus/ N.S.Garaeva, A. G. Bikmullin, Bruno Keiffer, Sh. Z. Validov, M.M. Yusupov, K.S. Usachev//Biomolecular NMR Assignments. - 2022. - V.16. - P.373-377.

Annotation

The ribosomal maturation factor (RimP) is a 17.7 kDa protein and is the assembly factor of the 30S subunit. RimP is essential for efficient processing of 16S rRNA and maturation (assembly) of the 30S ribosome. It was suggested that RimP takes part in stabilization of the central pseudoknot at the early stages of the 30S subunit maturation, and this process may occur before the head domain assembly and later stages of the 30S assembly, but the mechanism of this interaction is still not fully understood. Here we report the assignment of the 1H, 13C and 15N chemical shift in the backbone and side chains of RimP from Staphylococcus aureus. Analysis of chemical shifts of the main chain using TALOS + suggests that the RimP contains eight β-strands and three α-helices with the topology α1-β1-β2-α2- β3- α3- β4- β5- β6- β7- β8. Structural studies of RimP and its complex with the ribosome by integrated structural biology approaches (NMR spectroscopy, X-ray diffraction analysis and cryoelectron microscopy) will allow further screening of highly selective inhibitors of the translation of S. aureus.

Keywords

RimP, Ribosome maturation, Staphylococcus aureus, Ribosome, Protein NMR, TEV protease, Resonance assignment

The name of the journal

Biomolecular NMR Assignments

URL

https://www.researchgate.net/publication/363348888_Backbone_and_side_chain_NMR_assignments_for_the_ribosome_maturation_factor_P_RimP_from_Staphylococcus_aureus

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=272397&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Garaeva Nataliya Sergeevna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	N. S. Garaeva Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus/ N.S.Garaeva, A. G. Bikmullin, Bruno Keiffer, Sh. Z. Validov, M.M. Yusupov, K.S. Usachev//Biomolecular NMR Assignments. - 2022. - V.16. - P.373-377.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=272397&p_lang=2	ru_RU
dc.description.abstract	Biomolecular NMR Assignments	ru_RU
dc.description.abstract	The ribosomal maturation factor (RimP) is a 17.7 kDa protein and is the assembly factor of the 30S subunit. RimP is essential for efficient processing of 16S rRNA and maturation (assembly) of the 30S ribosome. It was suggested that RimP takes part in stabilization of the central pseudoknot at the early stages of the 30S subunit maturation, and this process may occur before the head domain assembly and later stages of the 30S assembly, but the mechanism of this interaction is still not fully understood. Here we report the assignment of the 1H, 13C and 15N chemical shift in the backbone and side chains of RimP from Staphylococcus aureus. Analysis of chemical shifts of the main chain using TALOS + suggests that the RimP contains eight β-strands and three α-helices with the topology α1-β1-β2-α2- β3- α3- β4- β5- β6- β7- β8. Structural studies of RimP and its complex with the ribosome by integrated structural biology approaches (NMR spectroscopy, X-ray diffraction analysis and cryoelectron microscopy) will allow further screening of highly selective inhibitors of the translation of S. aureus.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	RimP	ru_RU
dc.subject	Ribosome maturation	ru_RU
dc.subject	Staphylococcus aureus	ru_RU
dc.subject	Ribosome	ru_RU
dc.subject	Protein NMR	ru_RU
dc.subject	TEV protease	ru_RU
dc.subject	Resonance assignment	ru_RU
dc.title	Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus	ru_RU
dc.type	Articles in international journals and collections	ru_RU