KFU. Card publication. Isolation, culturing and 3D bioprinting equine myoblasts

KAZAN
FEDERAL UNIVERSITY

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- 中文
- ES

ISOLATION, CULTURING AND 3D BIOPRINTING EQUINE MYOBLASTS

Form of presentation

Articles in Russian journals and collections

Year of publication

2022

Язык

английский

Authors, employees of KFU

Aymaletdinov Aleksandr Maazovich, author

Zakirova Elena Yurevna, author

Rizvanov Albert Anatolevich, author

Other authors

Rutland Catrin , author

Authors, students KFU

Kurilov Igor Vitalevich, author

Yuferova Alina Konstantinovna, author

Bibliographic description in the original language

Aimaletdinov A. M. Isolation, culturing and 3D bioprinting equine myoblasts / A.M.Aimaletdinov, M. Abyzova, I. Kurilov, A. Yuferova, C. Rutland, A.A. Rizvanov, E.Yu. Zakirova // Biological Communications. - 2022. - № 67(3). - r.152–159.

Annotation

Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.

Keywords

myoblasts, bioprinting, microcarriers

The name of the journal

Biological Communications

URL

https://doi.org/10.21638/spbu03.2022.302

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=272340&p_lang=2

Resource files

File name	Size (MB)	Format
F_12821_Article_Text_47902_1_10_20221009.pdf	2,79	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Aymaletdinov Aleksandr Maazovich	ru_RU
dc.contributor.author	Zakirova Elena Yurevna	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Rutland Catrin	ru_RU
dc.contributor.author	Kurilov Igor Vitalevich	ru_RU
dc.contributor.author	Yuferova Alina Konstantinovna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Aimaletdinov A. М. Isolation, culturing and 3D bioprinting equine myoblasts / А.М.Aimaletdinov, М. Abyzova, I. Kurilov, A. Yuferova, C. Rutland, A.A. Rizvanov, E.Yu. Zakirova // Biological Communications. - 2022. - № 67(3). - р.152–159.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=272340&p_lang=2	ru_RU
dc.description.abstract	Biological Communications	ru_RU
dc.description.abstract	Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	myoblasts	ru_RU
dc.subject	bioprinting	ru_RU
dc.subject	microcarriers	ru_RU
dc.title	Isolation, culturing and 3D bioprinting equine myoblasts	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU