KFU. Card publication. Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode

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FEDERAL UNIVERSITY

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ELECTROCHEMICAL DNA SENSOR BASED ON ACRIDINE YELLOW ADSORBED ON GLASSY CARBON ELECTRODE

Form of presentation

Articles in international journals and collections

Year of publication

2021

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Authors, employees of KFU

Evtyugin Gennadiy Arturovich, author

Kulikova Tatyana Nikolaevna, author

Porfireva Anna Veniaminovna, author

Rogov Aleksey Mikhaylovich, author

Bibliographic description in the original language

Kulikova T., Porfireva A., Rogov A., Evtugyn G. (2021) Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode. Sensors, 21(22):7763. https://doi.org/10.3390/s21227763

Annotation

Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies

Keywords

Carbon black, Cyclic voltammetry, Doxorubicin determination, Drug determination, Electrochemical DNA sensor

The name of the journal

Sensors

URL

https://doi.org/10.3390/s21227763

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=272319&p_lang=2

Resource files

File name	Size (MB)	Format
sensors_21_07763.pdf	2,49	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Evtyugin Gennadiy Arturovich	ru_RU
dc.contributor.author	Kulikova Tatyana Nikolaevna	ru_RU
dc.contributor.author	Porfireva Anna Veniaminovna	ru_RU
dc.contributor.author	Rogov Aleksey Mikhaylovich	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Kulikova T., Porfireva A., Rogov A., Evtugyn G. (2021) Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode. Sensors, 21(22):7763. https://doi.org/10.3390/s21227763	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=272319&p_lang=2	ru_RU
dc.description.abstract	Sensors	ru_RU
dc.description.abstract	Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Carbon black	ru_RU
dc.subject	Cyclic voltammetry	ru_RU
dc.subject	Doxorubicin determination	ru_RU
dc.subject	Drug determination	ru_RU
dc.subject	Electrochemical DNA sensor	ru_RU
dc.title	Electrochemical DNA sensor based on acridine yellow adsorbed on glassy carbon electrode	ru_RU
dc.type	Articles in international journals and collections	ru_RU