KFU. Card publication. Effect of N,N-?dimethylacetamide (DMAA) on the-viability of cell lines SKOV-3, OVCAR-8 during cryopreservation

KAZAN
FEDERAL UNIVERSITY

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EFFECT OF N,N-?DIMETHYLACETAMIDE (DMAA) ON THE-VIABILITY OF CELL LINES SKOV-3, OVCAR-8 DURING CRYOPRESERVATION

Form of presentation

Conference proceedings in international journals and collections

Year of publication

2022

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Authors, employees of KFU

Abramova Zinaida Ivanovna, author

Bibliographic description in the original language

Ibragimov B. 56ASM-0211 | Effect of N,N-?dimethylacetamide (DMAA) on the-viability of cell lines SKOV-3, OVCAR-8 during cryopreservation /B. Ibragimov, D. Nedorezova, V. Yugai, Z. Abramova / 56th Annual scientific meeting, 8–10 June 2022, Bari, Italy// Eur J Clin Invest.-2022.-V.52, Is.S1.- P.169-170,

Annotation

56th Annual scientific meeting

Keywords

N,N-?dimethylacetamide, SKOV-3, OVCAR-8, cryopreservation

The name of the journal

56th Annual scientific meeting

URL

https://onlinelibrary.wiley.com/doi/full/10.1111/eci.13796

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=267659&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Ibragimov B. 56ASM-0211 \| Effect of N,N-?dimethylacetamide (DMAA) on the-viability of cell lines SKOV-3, OVCAR-8 during cryopreservation /B. Ibragimov, D. Nedorezova, V. Yugai, Z. Abramova / 56th Annual scientific meeting, 8–10 June 2022, Bari, Italy// Eur J Clin Invest.-2022.-V.52, Is.S1.- P.169-170,	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=267659&p_lang=2	ru_RU
dc.description.abstract	56th Annual scientific meeting	ru_RU
dc.description.abstract	An improvement of technologies for controlling the processes of cell life activity under low-temperature conditions is one of the most important direc-tions in the development of modern medicine and biology. The methods of vitrification allow to preserve the cells structure and the subtle mechanisms of their functioning. Therefore, these methods provide the possibilities to use a tumor cell cryobiobank for monitoring the process of the patient's treatment. This work is aimed at analyzing the effect of cryoprotectants, such as N,N- dimethylacetamide (DMAA), glycerol, dimethyl sulfoxide (DMSO), and a mixture of glycerol- DMAA, on ovarian carcinoma cell lines (SKOV-3, OVCAR-8) by determining the IC50 of these cryopreservatives for these cell lines. The results can be used in the future for the development of cryoprotec-tive mixtures, which will be less toxic. Purpose of the work: to provide the comparative assess-ment of the effect of DMAA, glycerol, DMSO and a mix-ture of DMAA-glycerol solutions on the viability of cell lines SKOV- 3, OVCAR- 8. Experimental data show that the concentration of half- maximal cell growth inhibition (IC50) of the mix-ture of DMAA and glycerol solutions was higher in both cell lines compared to the IC50 of the original solution. Accordingly, cells treated with this mixture had a higher viability. The results indicate that cryoprotectants, which make cells less susceptible to cryodamage, reduce the intensity of cell vital processes. A mixture of cryoprotectants DMAA and glycerol has less toxicity than the original DMAA solution.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	N	ru_RU
dc.subject	N-?dimethylacetamide	ru_RU
dc.subject	SKOV-3	ru_RU
dc.subject	OVCAR-8	ru_RU
dc.subject	cryopreservation	ru_RU
dc.title	Effect of N,N-?dimethylacetamide (DMAA) on the-viability of cell lines SKOV-3, OVCAR-8 during cryopreservation	ru_RU
dc.type	Conference proceedings in international journals and collections	ru_RU