KFU. Card publication. The Nuclease from Gram-Negative Bacteria Serratia Marcescens is Weakly Cytotoxic at Therapeutic Doses

KAZAN
FEDERAL UNIVERSITY

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THE NUCLEASE FROM GRAM-NEGATIVE BACTERIA SERRATIA MARCESCENS IS WEAKLY CYTOTOXIC AT THERAPEUTIC DOSES

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Malaneva Albina Gennadevna, author

Rizvanov Albert Anatolevich, author

Salikhova Taliya Ilshatovna, author

Filimonova Mariya Nikolaevna, author

Bibliographic description in the original language

Malanyeva A.G. The Nuclease from Gram-Negative Bacteria Serratia Marcescens is Weakly Cytotoxic at Therapeutic Doses/ A. G. Malanyeva , T. Salikhova , A.A . Rizvanov, M.N. Filimonova // Open Access Journal of Veterinary Science & Research.-2020.-V.5.-#2.-P.1-8

Annotation

Open Access Journal of Veterinary Science & Research

Keywords

Serratia Marcescens nuclease, Sma Nuc, Isoforms Sm1 And Sm2, Cytotoxity, Pulmozyme, Anti-Rabies Activity, MTT-Test, Human Colorectal Cancer Cell Culture, Human Breast Cell Cancer, Human Skin Fibroblasts

The name of the journal

Open Access Journal of Veterinary Science & Research

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=267208&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Malaneva Albina Gennadevna	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Salikhova Taliya Ilshatovna	ru_RU
dc.contributor.author	Filimonova Mariya Nikolaevna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Malanyeva A.G. The Nuclease from Gram-Negative Bacteria Serratia Marcescens is Weakly Cytotoxic at Therapeutic Doses/ A. G. Malanyeva , T. Salikhova , A.A . Rizvanov, M.N. Filimonova // Open Access Journal of Veterinary Science & Research.-2020.-V.5.-#2.-P.1-8	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=267208&p_lang=2	ru_RU
dc.description.abstract	Open Access Journal of Veterinary Science & Research	ru_RU
dc.description.abstract	The nuclease from S.marcescens heads a family of homological nonspecific nucleases. Its cultivation features, structure, mechanism of action, some physical, chemical and biochemical properties are well studied. It is presented by two isoforms, differing by N-terminal fragment and some properties. The nuclease demonstrated a cancer suppressing effect in mice. It is a key component of the anti-rabies composition which increases survival of the infected mice. It is similar by its efficiency to Pulmozyme? that is used for airway cleansing during the treatment of pulmonary diseases. The aim of the study was to analyze a cytotoxic effect of S. marcescens nuclease and its separate isoforms at different concentrations. To determine the cytotoxicity we used MTT assay and human cell cultures of colorectal cancer -HCT 116, breast cancer MCF-7 and normal skin fibroblasts. Results show that the nuclease demonstrates a weak cytotoxic effect at the amounts of 0.2 – 25 μg per 1 ml of the cell culture medium that is close to its therapeutic doses. This effect is not connected with the loss of the enzymatic activity. The isoforms are similar by their IC50.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Serratia Marcescens nuclease	ru_RU
dc.subject	Sma Nuc	ru_RU
dc.subject	Isoforms Sm1 And Sm2	ru_RU
dc.subject	Cytotoxity	ru_RU
dc.subject	Pulmozyme	ru_RU
dc.subject	Anti-Rabies Activity	ru_RU
dc.subject	MTT-Test	ru_RU
dc.subject	Human Colorectal Cancer Cell Culture	ru_RU
dc.subject	Human Breast Cell Cancer	ru_RU
dc.subject	Human Skin Fibroblasts	ru_RU
dc.title	The Nuclease from Gram-Negative Bacteria Serratia Marcescens is Weakly Cytotoxic at Therapeutic Doses	ru_RU
dc.type	Articles in international journals and collections	ru_RU