KFU. Card publication. Polyelectrolyte Polyethylenimine-DNA Complexes in the Composition of Voltammetric Sensors for Detecting DNA Damage

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

POLYELECTROLYTE POLYETHYLENIMINE-DNA COMPLEXES IN THE COMPOSITION OF VOLTAMMETRIC SENSORS FOR DETECTING DNA DAMAGE

Form of presentation

Articles in international journals and collections

Year of publication

2022

��

��

Authors, employees of KFU

Evtyugin Gennadiy Arturovich, author

Ziyatdinova Guzel Kamilevna, author

Ivanov Aleksey Nikolaevich, author

Kuzin Yuriy Ivanovich, author

Malanina Anastasiya Nikolaevna, author

Stoykov Ivan Ivanovich, author

Shurpik Dmitriy Nikolaevich, author

Authors, postgraduates KFU

Malanina Anastasiya Nikolaevna, postgraduate kfu

Bibliographic description in the original language

Malanina, A.N. Polyelectrolyte polyethylenimine-DNA complexes in the composition of voltammetric sensors for detecting DNA damage / A.N. Malanina, Yu. I. Kuzin, A.N. Ivanov, G.K. Ziyatdinova, D.N. Shurpik, I.I. Stoikov, G.A. Evtugyn // J. Anal. Chem. - 2022. - V. 77. - � 2. - P. 185-194.

Annotation

A voltammetric DNA sensor based on the polyelectrolyte complexes of polyethylenimine and DNA obtained by self-assembly from solutions of components on a glassy carbon electrode has been developed. Cyclic voltammetry was used to study the preparation conditions and characterize the permeability of the complex for low-molecular-weight redox indicators differing in the mechanism of interaction with DNA (methylene blue and methylene green). The formation of the polyelectrolyte complex was independently confirmed by surface plasmon resonance measurements. The redox peak currents of methylene blue and methylene green varied differently depending on the number of layers of the complex and the nature of the outer layer due to different mechanisms of binding the indicators to DNA molecules and varied assembly density of the complex polyelectrolytes. An increase in the concentration of polyions from 0.1 to 10 μg/mL suppressed the effect of the charge of the outer layer on the indicator currents. Polyelectrolyte coatings were tested to detect specific DNA interactions including intercalation with the anticancer drug doxorubicin and the thermal and chemical denaturation of DNA. It was found that the introduction of decacarboxylated pillar[5]arene into the layer increased the sensitivity of the methylene blue signal to the thermal denaturation of DNA. A comparison between changes in the peak currents of methylene blue and methylene green makes it possible to distinguish the mechanism of DNA damage and separately take into account the contribution of the considered chemical and physical damaging factors. The developed DNA sensor makes it possible to semiquantitatively evaluate the protective effect of antioxidants, which decrease the effect of reactive oxygen species on DNA in the biosensor.

Keywords

DNA sensor, polyelectrolyte complexes, voltammetry, oxidative DNA damage, methylene blue, methylene green

The name of the journal

JOURNAL OF ANALYTICAL CHEMISTRY

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125589325&doi=10.1134%2fS1061934822020095&partnerID=40&md5=36b5b205a5f6e263396a17ee82f73b56

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=264199&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Evtyugin Gennadiy Arturovich	ru_RU
dc.contributor.author	Ziyatdinova Guzel Kamilevna	ru_RU
dc.contributor.author	Ivanov Aleksey Nikolaevich	ru_RU
dc.contributor.author	Kuzin Yuriy Ivanovich	ru_RU
dc.contributor.author	Malanina Anastasiya Nikolaevna	ru_RU
dc.contributor.author	Stoykov Ivan Ivanovich	ru_RU
dc.contributor.author	Shurpik Dmitriy Nikolaevich	ru_RU
dc.contributor.author	Malanina Anastasiya Nikolaevna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Malanina, A.N. Polyelectrolyte polyethylenimine-DNA complexes in the composition of voltammetric sensors for detecting DNA damage / A.N. Malanina, Yu. I. Kuzin, A.N. Ivanov, G.K. Ziyatdinova, D.N. Shurpik, I.I. Stoikov, G.A. Evtugyn // J. Anal. Chem. - 2022. - V. 77. - � 2. - P. 185-194.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=264199&p_lang=2	ru_RU
dc.description.abstract	JOURNAL OF ANALYTICAL CHEMISTRY	ru_RU
dc.description.abstract	A voltammetric DNA sensor based on the polyelectrolyte complexes of polyethylenimine and DNA obtained by self-assembly from solutions of components on a glassy carbon electrode has been developed. Cyclic voltammetry was used to study the preparation conditions and characterize the permeability of the complex for low-molecular-weight redox indicators differing in the mechanism of interaction with DNA (methylene blue and methylene green). The formation of the polyelectrolyte complex was independently confirmed by surface plasmon resonance measurements. The redox peak currents of methylene blue and methylene green varied differently depending on the number of layers of the complex and the nature of the outer layer due to different mechanisms of binding the indicators to DNA molecules and varied assembly density of the complex polyelectrolytes. An increase in the concentration of polyions from 0.1 to 10 μg/mL suppressed the effect of the charge of the outer layer on the indicator currents. Polyelectrolyte coatings were tested to detect specific DNA interactions including intercalation with the anticancer drug doxorubicin and the thermal and chemical denaturation of DNA. It was found that the introduction of decacarboxylated pillar[5]arene into the layer increased the sensitivity of the methylene blue signal to the thermal denaturation of DNA. A comparison between changes in the peak currents of methylene blue and methylene green makes it possible to distinguish the mechanism of DNA damage and separately take into account the contribution of the considered chemical and physical damaging factors. The developed DNA sensor makes it possible to semiquantitatively evaluate the protective effect of antioxidants, which decrease the effect of reactive oxygen species on DNA in the biosensor.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	DNA sensor	ru_RU
dc.subject	polyelectrolyte complexes	ru_RU
dc.subject	voltammetry	ru_RU
dc.subject	oxidative DNA damage	ru_RU
dc.subject	methylene blue	ru_RU
dc.subject	methylene green	ru_RU
dc.title	Polyelectrolyte Polyethylenimine-DNA Complexes in the Composition of Voltammetric Sensors for Detecting DNA Damage	ru_RU
dc.type	Articles in international journals and collections	ru_RU