KFU. Card publication. Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract

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IDENTIFICATION OF GENES INVOLVED IN THE FORMATION OF SOLUBLE DIETARY FIBER IN WINTER RYE GRAIN AND THEIR EXPRESSION IN CULTIVARS WITH DIFFERENT VISCOSITIES OF WHOLE MEAL WATER EXTRACT

Form of presentation

Articles in international journals and collections

Year of publication

2022

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Authors, employees of KFU

Ponomareva Mira Leonidovna, author

Bibliographic description in the original language

Kozlova L.V. Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract / L.V. Kozlova, A.R. Nazipova, O.V. Gorshkov, L.F. Gilmullina, O.V. Sautkina, N.V. Petrova, O.I. Trofimova, S.N. Ponomarev, M.L. Ponomareva, T.A. Gorshova // The Crop Journal - 2022. V.10. I. 2 - Pp. 532-549

Annotation

The grain of rye (Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides, making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract. However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of mRNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides, whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes

Keywords

Rye (Secale cereale) Kernel development Arabinoxylan Mixed-linkage glucan Viscosity

The name of the journal

The Crop Journal

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=261645&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ponomareva Mira Leonidovna	ru_RU
dc.date.accessioned	2022-01-01T00:00:00Z	ru_RU
dc.date.available	2022-01-01T00:00:00Z	ru_RU
dc.date.issued	2022	ru_RU
dc.identifier.citation	Kozlova L.V. Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract / L.V. Kozlova, A.R. Nazipova, O.V. Gorshkov, L.F. Gilmullina, O.V. Sautkina, N.V. Petrova, O.I. Trofimova, S.N. Ponomarev, M.L. Ponomareva, T.A. Gorshova // The Crop Journal - 2022. V.10. I. 2 - Pp. 532-549	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=261645&p_lang=2	ru_RU
dc.description.abstract	The Crop Journal	ru_RU
dc.description.abstract	The grain of rye (Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides, making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract. However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of mRNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides, whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of whole meal water extract	ru_RU
dc.type	Articles in international journals and collections	ru_RU