Kazan (Volga region) Federal University, KFU
KAZAN
FEDERAL UNIVERSITY
 
MORPHO-BIOCHEMICAL CHARACTERISTICS OF AUTOPHAGY OF LYMPHOCYTES FROM PERIPHERAL BLOOD OF PATIENTS WITH MODERATE AND SEVERE BRONCHIAL ASTHMA
Form of presentationArticles in international journals and collections
Year of publication2021
Языкандийский
  • Abramov Sergey Nikolaevich, author
  • Abramova Zinaida Ivanovna, author
  • Evtyugin Vladimir Gennadevich, author
  • Reshetnikova Irina Dmitrievna, author
  • Skibo Yuliya Valerevna, author
  • Vodounon Cyrille A -, author
  • Bibliographic description in the original language Skibo. Y. V. Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma/ Y.V. Skibo, Cy.A. Vodounon, B. B. Legba, S. N. Abramov, V. G. Evtugyn, I. D. Reshetnikova, Zi. I. Abramova //American Journal of Biomedical Research.-2021.-V.9, N. 1.-P.10-18
    Annotation The increased interest in the process of autophagy in various physiological and pathological conditions is the focus of this study. Therefore, the aim was to assess the involvement of lymphocyte autophagy in the pathogenesis of bronchial asthma. Forty-five patients with mild persistent asthma, 45 patients with severe persistent asthma, and 45 normal healthy controls were involved in this study. Autophagy was evaluated based on the expression of microtubule-associated protein light chain 3 (LC3) by western blot, Fluorescence microscopy, and flow cytometry. Transmission Electron Microscopy was used to detect autophagosome. The results showed that autophagy was activated in T-cells of patients with mild and severe asthma compared to the normal healthy control. Stress conditions induced autophagy in T-cells of asthmatic patients with mild and severe form, but not in the control group. Dexamethasone treatment of T-cells stimulated apoptosis in mild asthma patients and in the control group but in the group with severe asthma, dexamethasone induced autophagy. Thus, autophagy could play an important role in the pathogenesis of asthma especially of severe asthma, and may contribute to the survival and the activation of T-lymphocyte in patients with severe asthma.
    Keywords autophagy, apoptosis, T lymphocytes, bronchial asthma
    The name of the journal American Journal of Biomedical Research
    URL https://pubs.sciepub.com/ajbr/9/1/3/index.html
    Please use this ID to quote from or refer to the card https://repository.kpfu.ru/eng/?p_id=252908&p_lang=2
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