KFU. Card publication. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro

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REAPPRAISAL OF ANOXIC SPREADING DEPOLARIZATION AS A TERMINAL EVENT DURING OXYGEN?GLUCOSE DEPRIVATION IN BRAIN SLICES IN VITRO

Form of presentation

Articles in international journals and collections

Year of publication

2021

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Authors, employees of KFU

Gaynutdinov Azat Robertovich, author

Mukhtarov Marat Rakhimzyanovich, author

Khazipov Rustem Narimanovich, author

Authors, postgraduates KFU

Yuzekaeva Elvira Razilevna, postgraduate kfu

Bibliographic description in the original language

Juzekaeva E. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro/ E. Juzekaeva, A. Gainutdinov, M. Mukhtarov, R. Khazipov // Scientific Reports.-2020. (doi.org/10.1038/s41598-020-75975-w)

Annotation

Anoxic spreading depolarization (aSD) has been hypothesized as a terminal event during oxygen? glucose deprivation (OGD) in submerged cortical slices in vitro. However, mechanical artifacts caused by aSD-triggered edema may introduce error in the assessment of neuronal viability. Here, using continuous patch-clamp recordings from submerged rat cortical slices, we first confirmed that vast majority of L4 neurons permanently lost their membrane potential during OGD-induced aSD. In some recordings, spontaneous transition from whole-cell to out-side out configuration occurred during or after aSD, and only a small fraction of neurons survived aSD with reperfusion started shortly after aSD. Secondly, to minimize artifacts caused by OGD-induced edema, cells were short-term patched following OGD episodes of various duration. Nearly half of L4 cells maintained membrane potential and showed the ability to spike-fire if reperfusion started less than 10 min after aSD.

Keywords

Anoxic spreading depolarization (aSD), oxygen?glucose deprivation (OGD), submerged rat cortical slices, ischemia, in vitro ischemia model

The name of the journal

SCIENTIFIC REPORTS

URL

https://doi.org/10.1038/s41598-020-75975-w

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=250013&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gaynutdinov Azat Robertovich	ru_RU
dc.contributor.author	Mukhtarov Marat Rakhimzyanovich	ru_RU
dc.contributor.author	Khazipov Rustem Narimanovich	ru_RU
dc.contributor.author	Yuzekaeva Elvira Razilevna	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Juzekaeva E. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro/ E. Juzekaeva, A. Gainutdinov, M. Mukhtarov, R. Khazipov // Scientific Reports.-2020. (doi.org/10.1038/s41598-020-75975-w)	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=250013&p_lang=2	ru_RU
dc.description.abstract	SCIENTIFIC REPORTS	ru_RU
dc.description.abstract	Anoxic spreading depolarization (aSD) has been hypothesized as a terminal event during oxygen? glucose deprivation (OGD) in submerged cortical slices in vitro. However, mechanical artifacts caused by aSD-triggered edema may introduce error in the assessment of neuronal viability. Here, using continuous patch-clamp recordings from submerged rat cortical slices, we first confirmed that vast majority of L4 neurons permanently lost their membrane potential during OGD-induced aSD. In some recordings, spontaneous transition from whole-cell to out-side out configuration occurred during or after aSD, and only a small fraction of neurons survived aSD with reperfusion started shortly after aSD. Secondly, to minimize artifacts caused by OGD-induced edema, cells were short-term patched following OGD episodes of various duration. Nearly half of L4 cells maintained membrane potential and showed the ability to spike-fire if reperfusion started less than 10 min after aSD.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Anoxic spreading depolarization (aSD)	ru_RU
dc.subject	oxygen?glucose deprivation (OGD)	ru_RU
dc.subject	submerged rat cortical slices	ru_RU
dc.subject	ischemia	ru_RU
dc.subject	in vitro ischemia model	ru_RU
dc.title	Reappraisal of anoxic spreading depolarization as a terminal event during oxygen?glucose deprivation in brain slices in vitro	ru_RU
dc.type	Articles in international journals and collections	ru_RU