KFU. Card publication. The PhoPQ Two-Component System Q12Is the Major Regulator of Cell Surface Properties, Stress Responses and Plant-Derived Substrate Utilisation During Development of Pectobacterium versatile-Host Plant Pathosystems

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THE PHOPQ TWO-COMPONENT SYSTEM Q12IS THE MAJOR REGULATOR OF CELL SURFACE PROPERTIES, STRESS RESPONSES AND PLANT-DERIVED SUBSTRATE UTILISATION DURING DEVELOPMENT OF PECTOBACTERIUM VERSATILE-HOST PLANT PATHOSYSTEMS

Form of presentation

Articles in international journals and collections

Year of publication

2021

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Authors, employees of KFU

Gogolev Yuriy Viktorovich, author

Gogoleva Natalya Evgenevna, author

Other authors

Kravchenko Ulyana , author

Nikolaychik Evgeniy Arturovich, author

Bibliographic description in the original language

Kravchenko U., Gogoleva N., Kalubaka N., Kruk A., Diuba Y., Gogolev Y., Nikolaichik E. The PhoPQ Two-Component System Q12Is the Major Regulator of Cell Surface Properties, Stress Responses and Plant-Derived Substrate Utilisation During Development of Pectobacterium versatile-Host Plant Pathosystems // Front. Microbiol. – 2021. – V. 11. – P 3596. doi: 10.3389/fmicb.2020.621391

Annotation

Pectobacterium versatile (formerly P. carotovorum) is a recently defined species of soft rot enterobacteria capable of infecting many plant hosts and damaging different tissues. Complex transcriptional regulation of virulence properties can be expected for such a versatile pathogen. However, the relevant information is available only for related species and is rather limited. The PhoPQ two-component system, originally described in pectobacteria as PehRS, was previously shown to regulate a single gene, pehA. Using an insertional phoP mutant of Pectobacterium versatile (earlier—P. carotovorum), we demonstrate that PhoP regulates at least 115 genes with a majority of them specific for pectobacteria. The functions performed by PhoP-controlled genes include degradation, transport and metabolism of plant-derived carbon sources (polygalacturonate, arabinose-containing polysaccharides and citrate), modification of bacterial cell envelope and stress resistance. We also demonstrated PhoP involvement in establishing the order of plant cell wall decomposition and utilisation of the corresponding breakdown products. Based on experimental data and in silico analysis, we defined a PhoP binding site motif and provided proof for its universality in enteric bacteria. Scanning P. versatile genome for the locations of this motif suggested a much larger PhoP regulon enriched with the genes important for a plant pathogen, which makes PhoP a global virulence regulator. Potential PhoP targets include many regulatory genes and PhoP control over one of them, expI, was confirmed experimentally, highlighting the link between the PhoPQ two-component and quorum sensing systems. High concentrations of calcium and magnesium ions were found to abolish the PhoPQ-dependent transcription activation but did not relieve repression. Reduced PhoP expression and minimisation of PhoP dependence of regulon members' expression in P. versatile cells isolated from potato tuber tissues suggest that PhoPQ system is a key switch of expression levels of multiple virulence-related genes fine-tuned to control the development of P. versatile-host plant pathosystem.

Keywords

Pectobacterium, soft rot, PhoPQ regulon members, transcriptome, RNA-seq

The name of the journal

Frontiers in Microbiology

URL

https://www.frontiersin.org/articles/10.3389/fmicb.2020.621391/full

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=248035&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gogolev Yuriy Viktorovich	ru_RU
dc.contributor.author	Gogoleva Natalya Evgenevna	ru_RU
dc.contributor.author	Kravchenko Ulyana	ru_RU
dc.contributor.author	Nikolaychik Evgeniy Arturovich	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Kravchenko U., Gogoleva N., Kalubaka N., Kruk A., Diuba Y., Gogolev Y., Nikolaichik E. The PhoPQ Two-Component System Q12Is the Major Regulator of Cell Surface Properties, Stress Responses and Plant-Derived Substrate Utilisation During Development of Pectobacterium versatile-Host Plant Pathosystems // Front. Microbiol. – 2021. – V. 11. – P 3596. doi: 10.3389/fmicb.2020.621391	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=248035&p_lang=2	ru_RU
dc.description.abstract	Frontiers in Microbiology	ru_RU
dc.description.abstract	Pectobacterium versatile (formerly P. carotovorum) is a recently defined species of soft rot enterobacteria capable of infecting many plant hosts and damaging different tissues. Complex transcriptional regulation of virulence properties can be expected for such a versatile pathogen. However, the relevant information is available only for related species and is rather limited. The PhoPQ two-component system, originally described in pectobacteria as PehRS, was previously shown to regulate a single gene, pehA. Using an insertional phoP mutant of Pectobacterium versatile (earlier—P. carotovorum), we demonstrate that PhoP regulates at least 115 genes with a majority of them specific for pectobacteria. The functions performed by PhoP-controlled genes include degradation, transport and metabolism of plant-derived carbon sources (polygalacturonate, arabinose-containing polysaccharides and citrate), modification of bacterial cell envelope and stress resistance. We also demonstrated PhoP involvement in establishing the order of plant cell wall decomposition and utilisation of the corresponding breakdown products. Based on experimental data and in silico analysis, we defined a PhoP binding site motif and provided proof for its universality in enteric bacteria. Scanning P. versatile genome for the locations of this motif suggested a much larger PhoP regulon enriched with the genes important for a plant pathogen, which makes PhoP a global virulence regulator. Potential PhoP targets include many regulatory genes and PhoP control over one of them, expI, was confirmed experimentally, highlighting the link between the PhoPQ two-component and quorum sensing systems. High concentrations of calcium and magnesium ions were found to abolish the PhoPQ-dependent transcription activation but did not relieve repression. Reduced PhoP expression and minimisation of PhoP dependence of regulon members' expression in P. versatile cells isolated from potato tuber tissues suggest that PhoPQ system is a key switch of expression levels of multiple virulence-related genes fine-tuned to control the development of P. versatile-host plant pathosystem.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Pectobacterium	ru_RU
dc.subject	soft rot	ru_RU
dc.subject	PhoPQ regulon members	ru_RU
dc.subject	transcriptome	ru_RU
dc.subject	RNA-seq	ru_RU
dc.title	The PhoPQ Two-Component System Q12Is the Major Regulator of Cell Surface Properties, Stress Responses and Plant-Derived Substrate Utilisation During Development of Pectobacterium versatile-Host Plant Pathosystems	ru_RU
dc.type	Articles in international journals and collections	ru_RU