KFU. Card publication. Rye snow mold-associated Microdochium nivale strains inhabiting a common area: variability in genetics, morphotype, extracellular enzymatic activities and virulence

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RYE SNOW MOLD-ASSOCIATED MICRODOCHIUM NIVALE STRAINS INHABITING A COMMON AREA: VARIABILITY IN GENETICS, MORPHOTYPE, EXTRACELLULAR ENZYMATIC ACTIVITIES AND VIRULENCE

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Ponomareva Mira Leonidovna, author

Bibliographic description in the original language

Gorshkov V.Y. Rye snow mold-associated Microdochium nivale strains inhabiting a common area: variability in genetics, morphotype, extracellular enzymatic activities and virulence / V.Y. Gorshkov, E.V. Osipova, M.L. Ponomareva, S.N. Ponomarev, N.E. Gogoleva, O.E. Petrova, O.A. Gogoleva, A.R. Meshcherov, A.S. Balkin, E.P. Vetchinkina, K.O. Potapov, Y.V. Gogolev, V.N. Korzun // Journal of Fungi. - 2020. - 6 (4). - 335

Annotation

Snowmold is a severe plant disease caused by psychrophilic or psychrotolerant fungi, ofwhich Microdochium species are the most harmful. A clear understanding ofMicrodochium biology has many gaps; the pathocomplex and its dynamic are poorly characterized, virulence factors are unknown, genome sequences are not available, and the criteria of plant snow mold resistance are not elucidated. Our study aimed to identify comprehensive characteristics of a local community of snow mold-causing Microdochium species colonizing a particular crop culture. By using the next-generation sequencing (NGS) technique, we characterized fungal and bacterial communities of pink snow mold-affected winter rye (Secale cereale) plants within a given geographical location shortly after snowmelt. Twenty-one strains ofM. nivale were isolated, classified on the basis of internal transcribed spacer 2 (ITS2) region, and characterized by morphology, synthesis of extracellular enzymes, and virulence. Several types of extracellular enzymatic activities, the level of which had no correlations with the degree of virulence, were revealed for Microdochium species for the first time. Our study shows that genetically and phenotypically diverseM. nivale strains simultaneously colonize winter rye plants within a common area, and each strain is likely to utilize its own, unique strategy to cause the disease using “a personal” pattern of extracellular enzymes.

Keywords

plant?microbe interactions; Microdochium nivale; snow mold; plant cell-wall-degrading enzymes; virulence

The name of the journal

Journal of Fungi

URL

https://www.mdpi.com/2309-608X/6/4

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=244234&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ponomareva Mira Leonidovna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Gorshkov V.Y. Rye snow mold-associated Microdochium nivale strains inhabiting a common area: variability in genetics, morphotype, extracellular enzymatic activities and virulence / V.Y. Gorshkov, E.V. Osipova, M.L. Ponomareva, S.N. Ponomarev, N.E. Gogoleva, O.E. Petrova, O.A. Gogoleva, A.R. Meshcherov, A.S. Balkin, E.P. Vetchinkina, K.O. Potapov, Y.V. Gogolev, V.N. Korzun // Journal of Fungi. - 2020. - 6 (4). - 335	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=244234&p_lang=2	ru_RU
dc.description.abstract	Journal of Fungi	ru_RU
dc.description.abstract	Snowmold is a severe plant disease caused by psychrophilic or psychrotolerant fungi, ofwhich Microdochium species are the most harmful. A clear understanding ofMicrodochium biology has many gaps; the pathocomplex and its dynamic are poorly characterized, virulence factors are unknown, genome sequences are not available, and the criteria of plant snow mold resistance are not elucidated. Our study aimed to identify comprehensive characteristics of a local community of snow mold-causing Microdochium species colonizing a particular crop culture. By using the next-generation sequencing (NGS) technique, we characterized fungal and bacterial communities of pink snow mold-affected winter rye (Secale cereale) plants within a given geographical location shortly after snowmelt. Twenty-one strains ofM. nivale were isolated, classified on the basis of internal transcribed spacer 2 (ITS2) region, and characterized by morphology, synthesis of extracellular enzymes, and virulence. Several types of extracellular enzymatic activities, the level of which had no correlations with the degree of virulence, were revealed for Microdochium species for the first time. Our study shows that genetically and phenotypically diverseM. nivale strains simultaneously colonize winter rye plants within a common area, and each strain is likely to utilize its own, unique strategy to cause the disease using “a personal” pattern of extracellular enzymes.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Rye snow mold-associated Microdochium nivale strains inhabiting a common area: variability in genetics, morphotype, extracellular enzymatic activities and virulence	ru_RU
dc.type	Articles in international journals and collections	ru_RU