KFU. Card publication. Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis.

KAZAN
FEDERAL UNIVERSITY

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INACTIVATION OF THE GENERAL TRANS&SHY;CRIPTION FACTOR TNRA IN BACILLUS SUBTILIS BY PROTEOLYSIS.

Form of presentation

Articles in international journals and collections

Year of publication

2008

Authors, employees of KFU

Ilinskaya Olga Nikolaevna, author

Kayumov Ayrat Rashitovich, author

Sharipova Margarita Rashidovna, author

Other authors

Karl Forchhamer; Annette Heinrich

Bibliographic description in the original language

A. Kayumov, A. Heinrich, M. Sharipova, O. Iljinskaya, K. Forchhammer. Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis // Microbiology. - 2008. - V.154. - P.2348-2355. DOI 10.1099/mic.0.2008/019802-0.

Annotation

Under conditions of nitrogen limitation, the general transcription factor TnrA in Bacillus subtilis activates the expression of genes involved in assimilation of various nitrogen sources. Previously, TnrA activity has been shown to be controlled by protein-protein interaction with glutamine synthetase, the key enzyme of ammonia assimilation. Furthermore, depending on ATP and 2-oxoglutarate levels, TnrA can bind to the GlnK-AmtB complex. Here, we report that upon transfer of nitrate-grown ceils to combined nitrogen-depleted medium, TnrA is rapidly eliminated from the cells by proteolysis. As long as TnrA is membrane-bound through GlnK-AmtB interaction it seems to be protected from degradation. Upon removal of nitrogen sources, the localization of TnrA becomes cytosolic and degradation occurs. The proteolytic activity against TnrA was detected in the cytosolic fraction but not in the membrane, and its presence does not depend on the nitrogen regime of cell growth. The proteolytic degradation of TnrA as a response to complete nitrogen starvation might represent a novel mechanism of TnrA control in B. subtilis.

Keywords

Bacillus subtilis, transcription factor TnrA.

The name of the journal

MICROBIOL-SGM

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=24078&p_lang=2

Resource files

File name	Size (MB)	Format
microbiology_2008.pdf	1,10	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ilinskaya Olga Nikolaevna	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Sharipova Margarita Rashidovna	ru_RU
dc.date.accessioned	2008-01-01T00:00:00Z	ru_RU
dc.date.available	2008-01-01T00:00:00Z	ru_RU
dc.date.issued	2008	ru_RU
dc.identifier.citation	A. Kayumov, A. Heinrich, M. Sharipova, O. Iljinskaya, K. Forchhammer. Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis // Microbiology. - 2008. - V.154. - P.2348-2355. DOI 10.1099/mic.0.2008/019802-0.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=24078&p_lang=2	ru_RU
dc.description.abstract	MICROBIOL-SGM	ru_RU
dc.description.abstract	Under conditions of nitrogen limitation, the general transcription factor TnrA in Bacillus subtilis activates the expression of genes involved in assimilation of various nitrogen sources. Previously, TnrA activity has been shown to be controlled by protein-protein interaction with glutamine synthetase, the key enzyme of ammonia assimilation. Furthermore, depending on ATP and 2-oxoglutarate levels, TnrA can bind to the GlnK-AmtB complex. Here, we report that upon transfer of nitrate-grown ceils to combined nitrogen-depleted medium, TnrA is rapidly eliminated from the cells by proteolysis. As long as TnrA is membrane-bound through GlnK-AmtB interaction it seems to be protected from degradation. Upon removal of nitrogen sources, the localization of TnrA becomes cytosolic and degradation occurs. The proteolytic activity against TnrA was detected in the cytosolic fraction but not in the membrane, and its presence does not depend on the nitrogen regime of cell growth. The proteolytic degradation of TnrA as a response to complete nitrogen starvation might represent a novel mechanism of TnrA control in B. subtilis.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bacillus subtilis	ru_RU
dc.subject	transcription factor TnrA.	ru_RU
dc.title	Inactivation of the general transcription factor TnrA in Bacillus subtilis by proteolysis.	ru_RU
dc.type	Articles in international journals and collections	ru_RU