KFU. Card publication. Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application

- RUS
- 中文
- ES

MESO-SUBSTITUTED-BODIPY BASED FLUORESCENT BIOMARKER: SPECTRAL CHARACTERISTICS, PHOTOSTABILITY AND POSSIBILITIES FOR PRACTICAL APPLICATION

Form of presentation

Articles in international journals and collections

Year of publication

2020

��

��

Authors, employees of KFU

Islamov Daut Rinatovich, author

Kayumov Ayrat Rashitovich, author

Pavelev Roman Sergeevich, author

Usachev Konstantin Sergeevich, author

Bibliographic description in the original language

Guseva G.B, Antina E.V, Berezin M.B, Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application//Journal of Photochemistry and Photobiology A: Chemistry. - 2020. - Vol.401, Is.. - Art. � 112783.

Annotation

A fluorescent biomarker based on the boron(III) complex with meso-4-methoxycarbonylbutyl-substituted 3,3',5,5'-tetramethyl-2,2'-dipyrromethene (BODIPY) was synthesized. The BODIPY exhibits a large extinction coefficient (lgε ∼4.82–5.00) at 496–501 nm and high fluorescence quantum yield (∼77–100 %) in the blue-green region of the spectrum (509–518 nm). The maximal fluorescence quantum yield (φ) is observed in non-polar media (∼100 %) while φ slightly decreases to ∼90 % in alcohols (with the exception of octanol-1) and to ∼77 % in electron-donating dimethyl sulfoxide (DMSO). The BODIPY fluorophore demonstrates high photostability with the half-life of 41.4 and 91 h in toluene and cyclohexane, respectively. The proposed luminophore preferentially stains gram-positive bacteria and can be used for differential staining of gram-positive and gram-negative bacteria in mixed cultures. BODIPY also accumulates in the cytoplasm of the mammalian cells giving polar micro-speckled staining pattern which is more intensive in the tumor cells when compared to the fibroblasts. The pronounced affinity of BODIPY to mitochondria of eukaryotic cells could be used for specific staining of these organelles.

Keywords

BODIPY Fluorescent dyes Biomarker Spectral properties Photostability

The name of the journal

Journal of Photochemistry and Photobiology A: Chemistry

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088512805&doi=10.1016%2fj.jphotochem.2020.112783&partnerID=40&md5=552788931cef42745735ce329b4c80e0

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=237163&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Islamov Daut Rinatovich	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Pavelev Roman Sergeevich	ru_RU
dc.contributor.author	Usachev Konstantin Sergeevich	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Guseva G.B, Antina E.V, Berezin M.B, Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application//Journal of Photochemistry and Photobiology A: Chemistry. - 2020. - Vol.401, Is.. - Art. � 112783.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=237163&p_lang=2	ru_RU
dc.description.abstract	Journal of Photochemistry and Photobiology A: Chemistry	ru_RU
dc.description.abstract	A fluorescent biomarker based on the boron(III) complex with meso-4-methoxycarbonylbutyl-substituted 3,3',5,5'-tetramethyl-2,2'-dipyrromethene (BODIPY) was synthesized. The BODIPY exhibits a large extinction coefficient (lgε ∼4.82–5.00) at 496–501 nm and high fluorescence quantum yield (∼77–100 %) in the blue-green region of the spectrum (509–518 nm). The maximal fluorescence quantum yield (φ) is observed in non-polar media (∼100 %) while φ slightly decreases to ∼90 % in alcohols (with the exception of octanol-1) and to ∼77 % in electron-donating dimethyl sulfoxide (DMSO). The BODIPY fluorophore demonstrates high photostability with the half-life of 41.4 and 91 h in toluene and cyclohexane, respectively. The proposed luminophore preferentially stains gram-positive bacteria and can be used for differential staining of gram-positive and gram-negative bacteria in mixed cultures. BODIPY also accumulates in the cytoplasm of the mammalian cells giving polar micro-speckled staining pattern which is more intensive in the tumor cells when compared to the fibroblasts. The pronounced affinity of BODIPY to mitochondria of eukaryotic cells could be used for specific staining of these organelles.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application	ru_RU
dc.type	Articles in international journals and collections	ru_RU