Kazan (Volga region) Federal University, KFU
KAZAN
FEDERAL UNIVERSITY
 
MESO-SUBSTITUTED-BODIPY BASED FLUORESCENT BIOMARKER: SPECTRAL CHARACTERISTICS, PHOTOSTABILITY AND POSSIBILITIES FOR PRACTICAL APPLICATION
Form of presentationArticles in international journals and collections
Year of publication2020
Языканглийский
  • Islamov Daut Rinatovich, author
  • Kayumov Ayrat Rashitovich, author
  • Pavelev Roman Sergeevich, author
  • Usachev Konstantin Sergeevich, author
  • Bibliographic description in the original language Guseva G.B, Antina E.V, Berezin M.B, Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application//Journal of Photochemistry and Photobiology A: Chemistry. - 2020. - Vol.401, Is.. - Art. № 112783.
    Annotation A fluorescent biomarker based on the boron(III) complex with meso-4-methoxycarbonylbutyl-substituted 3,3',5,5'-tetramethyl-2,2'-dipyrromethene (BODIPY) was synthesized. The BODIPY exhibits a large extinction coefficient (lgε ∼4.82–5.00) at 496–501 nm and high fluorescence quantum yield (∼77–100 %) in the blue-green region of the spectrum (509–518 nm). The maximal fluorescence quantum yield (φ) is observed in non-polar media (∼100 %) while φ slightly decreases to ∼90 % in alcohols (with the exception of octanol-1) and to ∼77 % in electron-donating dimethyl sulfoxide (DMSO). The BODIPY fluorophore demonstrates high photostability with the half-life of 41.4 and 91 h in toluene and cyclohexane, respectively. The proposed luminophore preferentially stains gram-positive bacteria and can be used for differential staining of gram-positive and gram-negative bacteria in mixed cultures. BODIPY also accumulates in the cytoplasm of the mammalian cells giving polar micro-speckled staining pattern which is more intensive in the tumor cells when compared to the fibroblasts. The pronounced affinity of BODIPY to mitochondria of eukaryotic cells could be used for specific staining of these organelles.
    Keywords BODIPY Fluorescent dyes Biomarker Spectral properties Photostability
    The name of the journal Journal of Photochemistry and Photobiology A: Chemistry
    URL https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088512805&doi=10.1016%2fj.jphotochem.2020.112783&partnerID=40&md5=552788931cef42745735ce329b4c80e0
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